Macro-Prep 25 Q Resin

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Macro-Prep 25 Q Resin

Overview

Purification on Macro-Prep 25Q column

Separation of 360 µl of 6xHis-tagged dihydrofolate reductase E. coli lysate on a 2 ml column packed with macro-Prep 25 Q. Buffer A: 59 mM Trist, pH 8.3. Buffer B: 50 mM Trist, pH 8.3, 1 M NaCl; gradient: 0–100% B in 10 column volumes; flow rate: 2 ml/min.

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Macro-Prep 25 Q Strong Anion Exchange Resin offers high-resolution separations at high flow rates with medium pressures.
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Description

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Macro-Prep 25 Q Resin is a strong anion exchange resin that offers high-resolution separations at high flow rates with medium pressures. It possesses the same rigid, macroporous, and hydrophilic properties of 50 µm Macro-Prep High Q support but in a 25 µm bead that offers higher resolution.

Features and Benefits

  • Benchtop to pilot-scale separations, where maximum sample recovery and purity are important
  • Fewer chromatographic steps required
  • Removal of protein variants and other difficult contaminants that would require additional validation if present in the final formulation

More Information

Instruction Manual, Macro-Prep 25 Resins (Bulletin 4006129)


Specifications

Type of ion exchanger Strong anion
Functional group –N+(CH3)3
Total ionic capacity 220 ± 40 µeq/ml
Median particle size 25 µm
Nominal pore size 725 Å
Shipping counterion Cl-
Dynamic binding capacity >30 mg/ml BSA
Recommended linear flow rate range 50–300 cm/hr
Maximum linear flow rate 3,000 cm.hr 3,000 cm/hr
Chemical stability  
   1.0 M HCI >48 hr
   100% ethanol Excellent
   1.0 M HCI Excellent
pH stability 1–10
Volume changes  
   pH 4–10 <1%
   0.1–1.0 M NaCl <5%
Autoclavability Yes; do not autoclave the OH- form
Storage solution 20% ethanol

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Macro-Prep 25 Q Support

1530021
50 ml, strong anion exchange chromatography media, 25 µm particle size, for high-flow medium-pressure separations

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$775.00 
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Macro-Prep 25 Q Support

1530022
200 ml, strong anion exchange chromatography media, 25 µm particle size, for high-flow medium-pressure separations

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$2,782.00 
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(Discontinued) Macro-Prep 25 Q Support

153-0023
1 L, strong anion exchange chromatography media, 25 μm particle size

This product is no longer available. Please contact technical support for more information on replacement product.
Macro-Prep 25 Q Support

153-0024
5 L, strong anion exchange chromatography media, 25 μm particle size

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Kits & Reagents

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Protein Standard for Anion Exchange Chromatography

1250561
Pkg of 6 vials, lyophilized mixture of equine myoglobin, conalbumin, chicken ovalbumin, soybean trypsin inhibitor, for ion exchange chromatography

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$327.00 
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Documents

Number Description Options
2292 Macro-Prep Ion Exchange Supports Product Information Sheet, Rev A Click to download
4006129 Instruction Manual, Macro-Prep 25 Ion Exchange Resins, Ver C Click to download
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download

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