Macro-Prep® 25 Q Resin

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Macro-Prep 25 Q Resin

Overview

Purification on Macro-Prep 25Q column

Separation of 360 µl of 6xHis-tagged dihydrofolate reductase E. coli lysate on a 2 ml column packed with macro-Prep 25 Q. Buffer A: 59 mM Trist, pH 8.3. Buffer B: 50 mM Trist, pH 8.3, 1 M NaCl; gradient: 0–100% B in 10 column volumes; flow rate: 2 ml/min.

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Macro-Prep 25 Q Resin is a strong anion exchange resin that offers high-resolution separations at high flow rates with medium pressures. It possesses the same rigid, macroporous, and hydrophilic properties of 50 µm Macro-Prep High Q support but in a 25 µm bead that offers higher resolution.

Features and Benefits of Macro-Prep 25 Q Strong Anion Exchange Resin

  • Benchtop to pilot-scale separations, where maximum sample recovery and purity are important
  • Fewer chromatographic steps required
  • Removal of protein variants and other difficult contaminants that would require additional validation if present in the final formulation
More Information

Instruction Manual, Macro-Prep® 25 Resins (Bulletin 4006129)
Type of ion exchanger Strong anion
Functional group –N+(CH3)3
Total ionic capacity 220 ± 40 µeq/ml
Median particle size 25 µm
Nominal pore size 725 Å
Shipping counterion Cl-
Dynamic binding capacity >30 mg/ml BSA
Recommended linear flow rate range 50–300 cm/hr
Maximum linear flow rate 3,000 cm.hr 3,000 cm/hr
Chemical stability  
   1.0 M HCI >48 hr
   100% ethanol Excellent
   1.0 M HCI Excellent
pH stability 1–10
Volume changes  
   pH 4–10 <1%
   0.1–1.0 M NaCl <5%
Autoclavability Yes; do not autoclave the OH- form
Storage solution 20% ethanol
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Macro-Prep 25 Q Support

1530021
50 ml, strong anion exchange chromatography media, 25 µm particle size, for high-flow medium-pressure separations

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Macro-Prep 25 Q Support

1530022
200 ml, strong anion exchange chromatography media, 25 µm particle size, for high-flow medium-pressure separations

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(Discontinued) Macro-Prep 25 Q Support

153-0023
1 L, strong anion exchange chromatography media, 25 μm particle size

This product is no longer available. Please contact technical support for more information on replacement product.
Macro-Prep 25 Q Support

153-0024
5 L, strong anion exchange chromatography media, 25 μm particle size

3 1530024 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-0024_0921120652196.xml 1530024 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jthvldna 2017-11-14 07:08:01 true true internet/product_SKU jthvldna templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-0024_0921120652196.xml LSR 2019-05-04 20:55:59 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998734414 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:55:59 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-0024_0921120652196.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-0024_0921120652196.xml 1530024-macro-prep-25-q-support 1530024 Macro-Prep 25 Q Support Macro-Prep 25 Q Support 5 L, strong anion exchange chromatography media, 25 μm particle size 5 L, strong anion exchange chromatography media, 25 μm particle size 5 L, strong anion exchange chromatography media, 25 μm particle size /webroot/web/images/ps/products/ion_exchange_media/sku_thumb/global/153-0024_tn.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_view/global/153-0024_view.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_feature/global/153-0024_feature.jpg RepEx 153-0024 5 L, strong anion exchange chromatography media, 25 μm particle size 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Media N 0 1530024, macro-prep, 25, q, support, strong, anion, exchange, chromatography, media, 25um, particle, size, high-flow, medium-pressure, separation, protein, purification, liter, macroprep false null false $ null false false true true false USD true false true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1530024&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1530024&CartQuote=QT&quantity= 1256 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1530024&AddToDefaultWishList=true&quantity= 23151800 null 153-0024
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Protein Standard for Anion Exchange Chromatography

1250561
Pkg of 6 vials, lyophilized mixture of equine myoglobin, conalbumin, chicken ovalbumin, soybean trypsin inhibitor, for ion exchange chromatography

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Number Description Options
2292 Macro-Prep Ion Exchange Supports Product Information Sheet, Rev A Click to download
4006129 Instruction Manual, Macro-Prep 25 Ion Exchange Resins, Ver C Click to download
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download

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