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      Macro-Prep High S Resin

      156-0031.jpg

      Overview

      Macro-Prep High S strong cation exchange support contains sulfonate functional groups and is ideal for purifying basic and neutral proteins and peptides.
      Read full description Ordering Information

      Description

      Description

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      Macro-Prep High S Resin is a strong cation exchange resin containing sulfonate functional groups that is ideal for purifying basic and neutral proteins and peptides. It is an excellent choice for rapid purification that meets the demands of analytical, semipreparative, and process-scale applications.

      This methacrylate copolymer bead provides high-resolution separations at very high flow rates. Changes in pH or ionic strength of the buffer do not cause shrinking or swelling of the support. The superior mechanical and chemical stability of Macro-Prep High S Resin make it a preferred choice over other sulfonate cation exchangers on the market. Macro-Prep High S Resin can be sanitized with chaotropic agents and can be autoclaved. It provides consistent and reproducible results.

      Features and Benefits

      • High capacity for biomolecules
      • High resolution of complex biological mixtures
      • Rigid polymer matrix that allows high flow rates at moderate pressures
      • Hydrophilic matrix that reduces nonspecific binding
      • Macroporous beads that enhance access to ionic sites
      • High chemical, mechanical, and thermal stability

      More Information

      Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO 9001-registered corporation.

      Related Technologies

      Related Products

      Specifications

      Specifications

      Properties of Macro-Prep High S Support
       
      Type of support
      Strong cation exchange
      Functional group
      –SO3-
      Typical dynamic binding capacity*
      ≥49 lgG/ml
      Nominal particle size
      50 µm
      Nominal pore diameter
      1,000 Å
      Recommended maximum linear flow rate
      3,000 cm/hr
      Autoclavability
      30 min at 121°C
      pH stability
      1–10
      Regeneration
      in 1–2 M NaCl/KCI or 70% ethanol
      *Determined with human lgG.
       
      Chemical Compatability of Macro-Prep High S Support
       
      1% SDS
      Yes
      8 M guanidine-HCI
      Yes
      1 N HCI
      Yes
      100% ethanol
      Yes

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      156-0032_tn.jpg
      (Discontinued) Macro-Prep High S Support
      156-0032
      5 L, strong cation exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size
      156-0033_tn.jpg
      (Discontinued) Macro-Prep High S Support
      156-0033
      10 L, strong cation exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size

      Kits & Reagents

      Kits & Reagents

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      (Discontinued) Bio-Scale™ Mini Ion Exchange Sample Pack
      7324650
      Includes one 1 ml cartridge each of UNOsphere™ S, UNOsphere Q, Macro-Prep® High Q, Macro-Prep High S, Macro-Prep DEAE
      732-4130_tn.jpg
      (Discontinued) Bio-Scale Mini Macro-Prep High S Cartridges
      7324130
      Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
      The following replacement is recommended:
      • EconoFit Macro-Prep High S Columns, 5 x 1 ml ( 12009270 )
      732-4132_tn.jpg
      (Discontinued) Bio-Scale Mini Macro-Prep High S Cartridge
      7324132
      Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
      The following replacement is recommended:
      • EconoFit Macro-Prep High S Column, 1 x 5 ml ( 12009271 )
      732-4134_tn.jpg
      (Discontinued) Bio-Scale Mini Macro-Prep High S Cartridges
      7324134
      Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
      The following replacement is recommended:
      • EconoFit Macro-Prep High S Columns, 5 x 5 ml ( 12009272 )
      158-0100_tn.jpg
      (Discontinued) Media Sampler Pack
      1580100
      Includes 25 ml each of UNOsphere™ Q and UNOsphere S, Macro-Prep® DEAE, Macro-Prep High Q, and Macro-Prep High S; 5 ml of UNOsphere SUPrA™; 10 g each of CHT™ Ceramic Hydroxyapatite Types I and II, 40 μm; and 10 g of CFT™ Ceramic Fluoroapatite Type II, 40 μm
      125-0562_tn.jpg
      (Discontinued) Protein Standard for Cation Exchange Chromatography
      1250562
      Pkg of 6 vials, lyophilized mixture of equine myoglobin, Ribonuclease A, cytochrome c, for use in ion exchange chromatography

      Documents

      Documents

      Number Description Options
      LIT271
      Instruction Manual, Macro-Prep Ion Exchange Supports, Rev D
      5584
      Bio-Scale Mini Cartridges, Product Information Sheet, Rev B
      5643
      Macro-Prep® High S Support Product Information Sheet, Rev A
      6810
      Practical Guide: Selecting the Optimal Resins for the Process Purification of Native and Recombinant Proteins, Ver A
      6983
      Native and Recombinant Protein Purification Resins/Media Selection Card
      1985
      Clearance of Murine Leukemia Virus From a Chimeric Monoclonal Antibody Using Ion Exchange Chromatography, Rev B.
      1913
      Developing and Scaling Up a Purification Procedure With Two New Ion Exchange Supports (Poster), Rev B
      2026
      Evaluation of Different Approaches for the Chromatographic Purification of Monoclonal Antibodies
      2079
      Non-Protein A Based Purification of MAb 414, Rev B
      6868
      Macro-Prep® Chromatography Resins Publications List, Ver A
      5662
      Process Chromatography Applications Laboratory Brochure, Rev B