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Overview
500 μl, 17,500–76,000 MW range, pI 4.5–8.5, unstained mixture of myoglobin, trypsin inhibitor, CA, GAPDH, actin, BSA, and conalbumin
Safety Data Sheet
Description
Description
Use 2-D SDS-PAGE Standards for spot matching and molecular weight determination of proteins on 2-D PAGE gels. Our 2-D SDS-PAGE Standards are a mixture of seven proteins (17.5–76 kD, with pI 4.5–8.5). The seven reduced, denatured proteins can be visualized with silver or Coomassie Blue stains. No dilution is required.
Applications and Uses
- PI and MW determination of sample proteins
- 2-D gel matching
- An internal control to assess reproducibility
2-D SDS-PAGE Standards Specifications
| Size Range | Quantity | Recommended Loading Volume |
| 7 spots, 17.5–76.0 kD, with pI 4.5–8.5 |
~0.30 mg protein in 500 µl of 9 M urea, 5% 2-mercaptoethanol, 2% Bio-Lyte® 5/7 ampholyte | Mini gels: 2.5 µl (Coomassie R-250 stain); 0.5–2.5 µl (silver stain) |
Related Products
Precision Plus Protein™ Unstained Standards
Precision Plus Protein™ Prestained Standards
Precision Plus Protein™ WesternC™ Standards
Related Categories
Protein Electrophoresis and Blotting Products
Supporting Documents and Resources
- 2-D SDS-PAGE Standards Instruction Manual
