Overview
500 μl, 17,500–76,000 MW range, pI 4.5–8.5, unstained mixture of myoglobin, trypsin inhibitor, CA, GAPDH, actin, BSA, and conalbumin
Available Safety Data Sheets
Safety Data Sheet
Find Certificate of Analysis
Description
Description
Use 2-D SDS-PAGE Standards for spot matching and molecular weight determination of proteins on 2-D PAGE gels. Our 2-D SDS-PAGE Standards are a mixture of seven proteins (17.5–76 kD, with pI 4.5–8.5). The seven reduced, denatured proteins can be visualized with silver or Coomassie Blue stains. No dilution is required.
Applications and Uses
- PI and MW determination of sample proteins
- 2-D gel matching
- An internal control to assess reproducibility
2-D SDS-PAGE Standards Specifications
| Size Range | Quantity | Recommended Loading Volume |
| 7 spots, 17.5–76.0 kD, with pI 4.5–8.5 |
~0.30 mg protein in 500 µl of 9 M urea, 5% 2-mercaptoethanol, 2% Bio-Lyte® 5/7 ampholyte | Mini gels: 2.5 µl (Coomassie R-250 stain); 0.5–2.5 µl (silver stain) |
Related Products
Precision Plus Protein™ Unstained Standards
Precision Plus Protein™ Prestained Standards
Precision Plus Protein™ WesternC™ Standards
Related Categories
Protein Electrophoresis and Blotting Products
Supporting Documents and Resources
- 2-D SDS-PAGE Standards Instruction Manual
