Protein Ladders & Standards (Markers)

Precision Plus Protein Standards have the same gel migration patterns, with three high-intensity reference bands (25, 50, and 75 kD), and provide exceptional lot-to-lot molecular weight consistency.

		Dual Color Standards	Kaleidoscope Standards	Dual Xtra Standards	All Blue Standards
Bands		10	10	12	10
Colors		2	5	2	1
MW range		10–250 kD	10–250 kD	2–250 kD	10–250 kD
Band sharpness		High	High	High	High
Packaging options	Sample (5 applications)	1610374S	—	—	—
	50 applications	1610374	1610375	1610377	1610373
	250 applications	1610394	1610395	1610397	1610393

Prestained Natural Protein Standards

Prestained natural protein standards for SDS-PAGE and western blotting provide a quick and easy way to monitor protein separation during electrophoresis and to assess transfer efficiency on blots. Each lot of prestained protein standards is individually calibrated for estimating the MW of sample proteins.

Important information

Prestained Natural Protein Standards will soon be phased out. We recommend the Precision Plus Protein Prestained Recombinant Protein Standards above as replacements.

Unstained natural protein standards allow accurate MW determination with uniform band intensities on SDS-PAGE gels stained with Coomassie Blue or zinc. Precision Plus Protein Unstained Recombinant Protein Standards are Strep-tagged, enabling immunodetection and molecular weight determination on western blots.

		Precision Plus Protein Unstained Standards*	Broad-Range SDS-PAGE Standards	Low-Range SDS-PAGE Standards	Natural High-Range SDS-PAGE Standards	Natural Polypeptide SDS-PAGE Standards
Bands		10	9	6	5	6
MW range (Note: Actual weights may vary; lot-specific MWs are included with each vial.)		10–250 kD	6.5–210 kD	14–97 kD	45–200 kD	1.4–26.6 kD
Band sharpness		High	Low	Low	Low	Low
Packaging options	100 applications	1610363	1610317	1610304	1610303	1610326
	500 applications	1610396	—	—	—	—

* Recommended for use with Bio-Rad's TGX Stain-Free Precast Gels and TGX Stain-Free FastCast Acrylamide handcast gels.

Western blotting protein standards can be used for both fluorescent visualization and colormetric or chemiluminescent immunodetection on western blots. Precision Plus Protein WesternC Standards are combination protein standards containing ten prestained, Strep-tagged recombinant proteins, enabling both fluorescent visualization on gels and colorimetric or chemiluminescent western blot detection with StrepTactin-AP or StrepTactin-HRP Conjugate.

	Bands		10
	Colors		2
	MW range		10–250 kD
	Band sharpness		High
	Packaging options	50 applications	Standards only (1610376) Standards and StrepTactin-HRP Conjugate (1610385)
		250 applications	Standards only (1610399) Standards and StrepTactin-HRP Conjugate (1610398)

IEF and 2D protein standards are a mixture of native proteins with isoelectric points (pI) ranging from 4.45 to 9.6, providing reproducible pI calibration in native PAGE or agarose IEF gels. 2-D SDS-PAGE protein standards provide calibrated references for protein pI and molecular weight in the second dimension.

		Precision Plus Protein Prestained Plug Standards	2-D SDS-PAGE Standards	Isoelectric Focusing (IEF) Standards
Bands		10	7	9
Colors		1	—	1
MW range/pI range		10–250 kD	17.5–76 kD, pI 4.5–8.5	pI 4.45–9.6
Packaging options	24 applications	1610378	—	—
	Number of applications depends on staining method	—	1610320	1610310

* 2-D standards: use 2.5 µl per mini gel for Coomassie staining (200 applications) or 0.5–2.5 µl for silver staining (up to 1,000 applications); use 1.0–5.0 µl for full-length gels (16–20 cm) to yield 100 or up to 500 applications, respectively.
IEF standards: use 5 µl per mini gel for Coomassie staining or 0.5 µl for silver staining to yield 50 or 500 applications, respectively.