Western Blotting Protein Standards

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Overview

Precision Plus Protein™ WesternC™ Standards are prestained, broad-range protein standards that enable both fluorescent imaging and chemiluminescent or colorimetric detection when probed with StrepTactin-HRP Conjugates.
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Description

Description

Free Protein Standards Selection Magnet

Free Protein Standards Selection Magnet

Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple.

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Precision Plus Protein™ WesternC™ Protein Standards are prestained, broad-range protein standards that enable chemiluminescent detection when probed with StrepTactin-HRP conjugates, so the protein standard appears directly on a film or CCD image.

WesternC Standards for protein electrophoresis and blotting offer unsurpassed band sharpness, accurate MW determination, lot-to-lot consistency, and excellent blotting results. These protein standards contain ten highly purified recombinant proteins with molecular masses from 10 to 250 kD and include three high-intensity reference bands (25, 50, and 75 kD).

Features and Benefits of WesternC Western Blotting Protein Standards

  • Clean, sharp bands with MW confirmed by mass spectrometry
  • Highly accurate MW estimation, r2 values >0.99
  • Proprietary staining technology that provides batch-to-batch MW consistency and reproducible electrophoretic migration
  • Dual-color prestained bands (reference bands prestained pink, all others prestained blue)
  • Prestained bands for monitoring of electrophoresis progression and assessment of blotting efficiency
  • Strep-tag affinity peptide for detection and MW determination on western blots
  • Supplied as ready-to-use solution
  • Fluorescent detection — blue bands can be visualized with a 635 nm laser or red LED. Pink bands can be visualized with a 532 nm laser or green LED.

Specifications

Specifications

MW size range 
10–250 kD 
Stain type
Stained
Volume
250 µl
Number of applications 
50 
Loading buffer 
30% (w/v) glycerol, 2% SDS,
62.5 mM Tris, pH 6.8, 50 mM DTT,
5 mM EDTA, 0.02% NaN3,
0.01% bromophenol blue
Shelf life
12 months at –20°C

Ordering

Ordering

items

Use the filters below to refine results!

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1610385
250 μl Precision Plus Protein WesternC standards and 125 μl StrepTactin-HRP conjugate, 50 applications
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1610398
1.25 ml (5 x 250 µl) Precision Plus Protein WesternC Standards and 625 µl (5 x 125 µl) StrepTactin-HRP conjugate, 250 applications
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1610376
250 μl, pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications
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1610399
1.25 ml (5 x 250 µl), 10 pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 250 applications
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1610380
300 μl, StrepTactin and horseradish peroxidase (HRP) conjugate used for detection of Precision Plus Protein unstained standards, 150 applications
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1610381
125 μl, StrepTactin and horseradish peroxidase (HRP) conjugate used for detection of Precision Plus Protein unstained standards, 50 applications
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1610382
300 μl, StrepTactin and alkaline phosphatase (AP) conjugate used for detection of Precision Plus Protein unstained standards, 150 applications

Documents

Documents

Number Description Options
6533
Introducing Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits, Rev A
2895
Protein Blotting Guide, Ver C
6040
Electrophoresis Guide, Interactive PDF, Rev C
2032
Western Blotting Detection Reagents Brochure, Rev F
5763
Molecular Weight Estimation Using Precision Plus Protein™ WesternC™ Standards on Criterion™ Tris-HCl and Criterion XT Bis-Tris Gels, Rev B
5723
Increase Western Blot Throughput with Multiplex Fluorescent Detection, Rev B
5685
Effect of PMA on Phosphorylation of Cx43: A Quantitative Evaluation Using Blotting With Multiplex Fluorescent Detection, Rev B
5576
Molecular Weight Estimation and Quantitation of Protein Samples Using Precision Plus Protein WesternC Standards, the Immun-Star WesternC Chemiluminescent Detection Kit, and the Molecular Imager ChemiDoc XRS Imaging System, Rev C
5575
Comparison of Chemiluminescent Protein Standards Product Insert, Rev C
5572
Immun-Star WesternC Chemiluminescent Kit Product Information Sheet, Rev D
5561
Precision Plus Protein™ WesternC™ Standards, Rev E
4110000
Instruction Manual, Precision StrepTactin HRP-Conjugate and AP-Conjugate, Rev D
10008761
Instruction Manual, Precision Plus Protein™ WesternC Standards, Rev H
2414
The Little Book of Standards, Rev D
2998
Protein Standards Application Guide, Rev G
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