Western Blotting Protein Standards

Precision Plus Protein™ WesternC™ Standards are prestained, broad-range protein standards that enable both fluorescent imaging and chemiluminescent or colorimetric detection when probed with StrepTactin-HRP Conjugates.

Ordering Information

Overview

Precision Plus Protein™ WesternC™ Protein Standards are prestained, broad-range protein standards that enable chemiluminescent detection when probed with StrepTactin-HRP conjugates, so the protein standard appears directly on a film or CCD image.

WesternC Standards for protein electrophoresis and blotting offer unsurpassed band sharpness, accurate MW determination, lot-to-lot consistency, and excellent blotting results. These protein standards contain ten highly purified recombinant proteins with molecular masses from 10 to 250 kD and include three high-intensity reference bands (25, 50, and 75 kD).

Features and Benefits of WesternC Western Blotting Protein Standards

Clean, sharp bands with MW confirmed by mass spectrometry
Highly accurate MW estimation, r² values >0.99
Proprietary staining technology that provides batch-to-batch MW consistency and reproducible electrophoretic migration
Dual-color prestained bands (reference bands prestained pink, all others prestained blue)
Prestained bands for monitoring of electrophoresis progression and assessment of blotting efficiency
Strep-tag affinity peptide for detection and MW determination on western blots
Supplied as ready-to-use solution
Fluorescent detection — blue bands can be visualized with a 635 nm laser or red LED. Pink bands can be visualized with a 532 nm laser or green LED.

Related Technologies

Detection Methods

Related Products

Specifications

MW size range

10–250 kD

Stain type

Stained

Volume

250 µl

Number of applications

Loading buffer

30% (w/v) glycerol, 2% SDS,
62.5 mM Tris, pH 6.8, 50 mM DTT,
5 mM EDTA, 0.02% NaN₃,
0.01% bromophenol blue

Shelf life

12 months at –20°C

Ordering

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(Discontinued) Precision Plus Protein™ WesternC™ Blotting Standards, value pack, 50 applications

1610385

250 μl Precision Plus Protein WesternC standards and 125 μl StrepTactin-HRP conjugate, 50 applications

(Discontinued) Precision Plus Protein™ WesternC™ Blotting Standards, value pack, 250 applications

1610398

1.25 ml (5 x 250 µl) Precision Plus Protein WesternC Standards and 625 µl (5 x 125 µl) StrepTactin-HRP conjugate, 250 applications

(Discontinued) Precision Plus Protein™ WesternC™ Blotting Standards, 250 µl

1610376

250 μl, pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications

(Discontinued) Precision Plus Protein™ WesternC™ Blotting Standards, 1.25 ml

1610399

1.25 ml (5 x 250 µl), 10 pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 250 applications