Overview
QX Software for Digital PCR Systems
When connected to a ddPCR System, QX Software provides all necessary functionality to create, run, and analyze experiments on your samples.
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This QX200 EvaGreen Digital PCR Supermix is a 2x concentrated, ready-to-use universal mix that delivers maximum PCR efficiency, specificity, and sensitivity in Droplet Digital™ PCR (ddPCR™).
This supermix supports double-stranded DNA target detection following amplification using commercially available EvaGreen Assays.
Key Benefits
- Contains all components required for EvaGreen-based ddPCR except primers and template
- Enables double-stranded DNA detection — only primers are needed to amplify and detect product
- Optimized for use with Bio-Rad’s PrimePCR™ Assays
- Hot-start polymerase keeps enzyme inactive during sample droplet partitioning
Applications and Uses
- Absolute quantification
- Gene expression analysis
- Copy number detection
- DNA rearrangements
- Multiple target analysis
Packaging Options
| Number of 20 µl Reactions | Supermix Volume, ml | Catalog Number |
|---|---|---|
| 200 | 2 x 1 | 186-4033 |
| 500 | 5 x 1 | 186-4034 |
| 2,500 | 5 x 5 | 186-4035 |
| 5,000 | 10 x 5 | 186-4036 |
Additional Reagents
In addition to the QX200™ ddPCR™ EvaGreen Supermix, we offer these consumables for completing your digital PCR workflow:
Additional Equipment
This digital PCR supermix requires the use of the following instruments and accessories:
- QX200™ Droplet Generator
- QX200™ Droplet Reader
- PX1™ PCR Plate Sealer
- PTC Tempo Deepwell Thermal Cycler or C1000 Touch™ Thermal Cycler with 96 Deep–Well Reaction Module
Related Products
Bio-Rad offers additional digital PCR supermixes including:
More Information
The QX200 ddPCR EvaGreen Supermix is optimized for use with Droplet Generation Oil for EvaGreen on the QX200™ Droplet Digital™ PCR System.
Specifications
Ordering
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200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX600/QX200 Droplet Digital™ PCR System
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX600/QX200 Droplet Digital™ PCR System
2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX600/QX200 Droplet Digital™ PCR System
5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX600/QX200 Droplet Digital™ PCR System
Accessories
2 L (2 x 1 L), oil for use with droplet reader in the QX600/QX200 Droplet Digital™ PCR Systems
Pkg of 24, cartridges for use with the QX200 Droplet Generator
Pkg of 24, gaskets for use with QX200 Droplet Generator
5 pkg of 24 DG8 Cartridges, 5 pkg of 24 DG8 Gaskets, for use with the QX200 Droplet Generator
Equipment
Droplet Digital PCR System, includes droplet generator, droplet reader, computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications
Droplet generator for EvaGreen or probe-based digital PCR applications, for use with QX200™ Droplet Digital™ PCR System
Droplet reader for EvaGreen or probe-based digital PCR applications, for use with the QX200 Droplet Digital™ System
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive