QX200™ ddPCR™ EvaGreen Supermix

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Overview

This universal digital PCR mix enables double-stranded DNA detection following amplification using EvaGreen Assays.

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Description

Description

This QX200 EvaGreen Digital PCR Supermix is a 2x concentrated, ready-to-use universal mix that delivers maximum PCR efficiency, specificity, and sensitivity in Droplet Digital™ PCR (ddPCR™).

This supermix supports double-stranded DNA target detection following amplification using commercially available EvaGreen Assays.

Key Benefits

  • Contains all components required for EvaGreen-based ddPCR except primers and template
  • Enables double-stranded DNA detection — only primers are needed to amplify and detect product
  • Optimized for use with Bio-Rad’s PrimePCR™ Assays
  • Hot-start polymerase keeps enzyme inactive during sample droplet partitioning

Applications and Uses

  • Absolute quantification
  • Gene expression analysis
  • Copy number detection
  • DNA rearrangements
  • Multiple target analysis

Packaging Options

Number of 20 µl Reactions Supermix Volume, ml Catalog Number
200 2 x 1 186-4033
500 5 x 1 186-4034
2,500 5 x 5 186-4035
5,000 10 x 5 186-4036

Additional Reagents

In addition to the QX200™ ddPCR™ EvaGreen Supermix, we offer these consumables for completing your digital PCR workflow:

Additional Equipment

This digital PCR supermix requires the use of the following instruments and accessories:

Related Products

Bio-Rad offers additional digital PCR supermixes including:

More Information

The QX200 ddPCR EvaGreen Supermix is optimized for use with Droplet Generation Oil for EvaGreen on the QX200™ Droplet Digital™ PCR System.

Specifications

Specifications

Storage at –20°C
Up to 12 months (Refer to expiration date)
Storage at 4°C (after thawing)
Up to 2 weeks
Template compatibility
cDNA, genomic DNA, plasmid DNA

Ordering

Ordering

items

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qx200-ddpcr-evagreen-supermix-186-4033-thumb.jpg
1864033
200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR System
qx200-evagreen-ddpcr-supermix-186-4034-thumb.jpg
1864034

500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR System

qx200-evagreen-ddpcr-supermix-186-4035-thumb.jpg
1864035
2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR System
qx200-evagreen-ddpcr-supermix-186-4036-thumb.jpg
1864036
5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR System

Accessories

Accessories

qx200-droplet-generation-oil-for-evagreen-assays-186-4005-thumb.jpg
1864005
14 ml (2 x 7 ml), oil for use with the droplet generator in the QX200™ Droplet Digital™ PCR System
qx200-droplet-generation-oil-for-evagreen-assays-186-4006-thumb.jpg
1864006
70 ml (10 x 7 ml), oil for use with the droplet generator in the QX200™ Droplet Digital™ PCR System
qx100-ddpcr-buffer-control-kit-186-3052-thumb.jpg
1864052
9 ml (2 x 4.5 ml), 2x buffer, for use as a blank control with Droplet Generation Oil for EvaGreen
qx100-droplet-reader-oil-2l-186-3004-thumb.jpg
1863004
2 L (2 x 1 L), oil for use with droplet reader in the QX200™/QX100™ Droplet Digital™ PCR Systems
dg8-cartridges-for-qx100-qx200-droplet-generator-186-4008-thumb.jpg
1864008
Pkg of 24, cartridges for use with the QX200™/QX100™ Droplet Generator
qx100-dg8-gaskets-186-3009-thumb.jpg
1863009
Pkg of 24, gaskets for use with QX200™/QX100™ Droplet Generator
droplet-generator-cartridges-and-gaskets-186-4007-thumb.jpg
1864007
5 pkg of 24 DG8 Cartridges, 5 pkg of 24 DG8 Gaskets, for use with the QX200™/QX100™ Droplet Generator
pierceable-foil-heat-seal-181-4040-thumb.jpg
1814040
Pkg of 100, foil seals for PCR and QX200/QX100 Droplet Digital PCR System applications, for use with PX1 PCR Plate Sealer

Equipment

Equipment

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1864001
Droplet Digital PCR System, includes droplet generator, droplet reader, computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications
qx200-droplet-generator-186-4002-thumb.jpg
1864002
Droplet generator for EvaGreen or probe-based digital PCR applications, for use with QX200™ Droplet Digital™ PCR System
qx200-droplet-reader-186-4003-thumb.jpg
(Discontinued) QX200 Droplet Reader
1864003
Droplet reader for EvaGreen or probe-based digital PCR applications, for use with the QX200 Droplet Digital™ System
PX1-PCR-Plate-Sealer-SKU-181-4000-thumb.jpg
(Discontinued) PX1 PCR Plate Sealer
1814000
PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord
185-1196_tn.jpg
1851197
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive

Documents

Documents

Number Description Options
6473
QX200 ddPCR EvaGreen Supermix Flier, Rev A
10028376
QX200™ ddPCR™ EvaGreen Supermix Product Insert
10040717
QX200 Droplet Generation Oil for EvaGreen Product Insert, Rev C
6311
QX200 Droplet Digital PCR System Brochure, Rev C
[ Add to Cart (Free) ]
10031907
Instruction Manual, QX200™ Droplet Generator, Rev C
10031906
Instruction Manual, QX200 Droplet Reader and QuantaSoft Software