pGLO Bacterial Transformation Kit

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Overview

Teach the central dogma and genetic engineering using E. coli and a gene from the bioluminescent jellyfish Aequorea victoria. This classic pGLO kit follows the same procedure used by molecular biologists to create "designer proteins".

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Description

Description

Note: This kit can be modified to accommodate individual workstations in socially-distanced classrooms.
Download instructions for modification.

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Bio-Rad's pGLO Bacterial Transformation Kit is the classic kit for teaching the central dogma and the basics of genetic engineering. In this bacterial transformation lab activity, students use the pGLO plasmid to transform bacteria to express green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea victoria, which causes the bacteria to glow green under UV light. Explore the concept of DNA > RNA > Protein > Trait and the roles that external and internal factors play in gene regulation.

Features and Benefits

  • Hands-on genetic engineering — from handling plasmids to performing heat shock and plating the transformants, students perform the same transformation protocol used by scientists on a daily basis around the world
  • Visible, dramatic phenotype — bacteria transformed with pGLO glow a brilliant fluorescent green under UV light. Once students transform bacteria with pGLO, they never forget the central dogma of molecular biology: DNA > RNA > Protein > Trait — Green Fluorescence
  • Gene expression and gene regulation — bacteria transformed with pGLO show green fluorescence only when they are grown in the presence of arabinose. Use this experiment to illustrate that not all genes are expressed at the same time and that environmental factors control when gene expression takes place

Each pGLO Bacterial Transformation Kit:

  • Includes consumables and reagents for 8 student workstations, with up to 4 students per workstation
  • Supports lab activities designed for two 50-minute sessions

pGLO Resources for Teachers

Find the kit instruction manual, student quick guide, and more under the Documents tab of this page.


Bio-Rad’s Family of pGLO Bacterial Transformation and GFP Kits

Bio-Rad offers a selection of kits for teaching about pGLO bacterial transformation, gene expression, and the green fluoresecent protein (GFP).

pGLO Bacterial Transformation Kits

A selection of pGLO bacterial transformation kits to help you meet your specific learning objectives

pGLO Bacterial Transformation Kit pGLO Bacterial Transformation Kit for General Biology pGLO Bacterial Transformation and Inquiry Kit for AP Biology
Overview A laboratory skills-based approach to bacterial transformation; students follow a robust protocol for a well-controlled experiment while analyzing the details of the technique A highly scaffolded three-dimensional approach to bacterial transformation and gene expression; students design their own step-wise experiments and write and revise models and scientific explanations An inquiry-based approach to bacterial transformation through experimental optimization; students ask their own questions and conduct multiple experiments to refine the parameters of the technique
Primarily curriculum concepts
Gene expression, gene regulation, antibiotic resistance,
artificial selection, experimental design, laboratory skills
Workstations
8
12
8
Laboratory activities
1
2
5
Class periods
(50 min)
2
3–5
Flexible to fit a range of class periods

pGLO and Green Fluorescent Protein (GFP) Kits

pGLO extension activities for investigating the properties and purification of the green fluorescent protein (GFP)

pGLO SDS-PAGE Extension* Green Fluorescent Protein Chromatography Kit* Secrets of the Rainforest Kit
Overview Use protein electrophoresis (SDS-PAGE) to view the expression of proteins in pGLO bacteria Use chromatography to purify glowing green fluorescent protein from pGLO bacteria (GFP)  Simulate the drug discovery process in your classroom with a glowing protein (GFP)
Primary curriculum concepts Protein expression, electrophoresis Protein expression, protein structure and function, chromatography Cloning and screening bacterial libraries, protein purification, chromatography, process and ethics of drug development
Workstations
8
8
8

* Requires purchase of a pGLO bacterial transformation kit.

Specifications

Specifications

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
 
Plasmid (pGLO), lyophilized
20 μg
E. coli strain HB101 K-12, lyophilized
1 vial
LB nutrient broth, sterile
10 ml
LB nutrient agar powder
20 g
Ampicillin, lyophilized
20 mg
Arabinose, lyophilized
600 mg
Sterile transformation solution (CaCl2)
100 ml
Petri dishes, 60 mm, sterile
40
Inoculation loops, sterile
80
Microcentrifuge tubes, 2.0 ml, sterile, color-coded
60
Disposable plastic transfer pipets
50
Foam micro test tube holders
8
UV pen light
1

Ordering

Ordering

items

Use the filters below to refine results!

1660003EDU
Gene expression analysis classroom kit, includes GFP encoding plasmid, nutrient broth/powder, petri dishes, UV pen light, and more, for 32 students; education use only, download free manual

Refills

Refills

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1660555EDU
Consumable reagents for pGLO Bacterial Transformation Kits, includes pGLO Plasmid, E. coli, nutrient broth, transformation solution, ampicillin, arabinose; education use only
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(Discontinued) pGLO™ Plasmid
1660405EDU
20 μg, lyophilized DNA, plasmid encoding the Green Fluorescent Protein (GFP), for use with the pGLO Bacterial Transformation Kit (#166-0003EDU); education use only
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1660408EDU
Lyophilized bacterial strain; education use only
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(Discontinued) LB Nutrient Broth
1660421EDU
10 ml, sterile broth; education use only
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(Discontinued) Ampicillin
1660407EDU
30 mg, lyophilized reagent, antibiotic for use with the pGLO™ Bacterial Transformation Kit (#166-0003EDU); education use only
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(Discontinued) Arabinose
1660406EDU
600 mg, lyophilized reagent, gene expression activator for use with the pGLO™ Bacterial Transformation Kit (#166-0003EDU); education use only
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(Discontinued) LB Agar Powder
1660600EDU
20 g, makes forty 60 mm agar plates; education use only
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1660409EDU
15 ml, sterile CaCl2 solution, for use with the pGLO™ Bacterial Transformation Kit (#166-0003EDU); education use only
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1660540EDU
Instructional DVD for use with pGLO Bacterial Transformation Kit (#166-0003EDU)
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1660472EDU
500 g, makes one thousand
60 mm agar plates; education use only
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(Discontinued) Petri Dishes
1660470EDU
Pkg of 500, 60 mm x 15 mm, sterile; education use only
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(Discontinued) Inoculation Loops
1660471EDU
Pkg of 100, 10 μl, sterile; education use only
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1660479EDU
Pkg of 8, microcentrifuge tube rack, holds 12 tubes; education use only
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1660474EDU
Pkg of 500, sterile; education use only
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1660473EDU
Pkg of 600, 6 colors; education use only
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1660033EDU
Pkg of 1, instruction manual, for use with pGLO Bacterial Transformation Kit (#166-0003EDU); download manual now; education use only; limit 1 per customer

Documents

Documents

Number Description Options
LIT1660033EDU
Instructor and Student Guide, pGLO Bacterial Transformation Kit
3052
pGLO Bacterial Transformation Kit, Biotechnology Explorer, Quick Guide
7433
pGLO Plasmid Map and Sequence
6672
Map of AP-Aligned Bio-Rad Kits with Essential Knowledge
6673
Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories
6605
Map of AP-Aligned Bio-Rad Kits with Learning Objectives
6581
20 Questions to Master Inquiry - pGLO™ Bacterial Transformation Kit, Rev A
5891
Refresh Kit Components — Large Class/Multiple Class Preparation Guide
M1660023
Instructor Guide, Protein Electrophoresis of GFP: A pGLO Bacterial Transformation Kit Extension