Secrets of the Rainforest™ Kit

Overview

Kit contains sufficient materials for 8 student workstations. (2–4 students per workstation)
Module 1: Preparing a Bacterial Library
Bacterial library, E. coli, lyophilized	1
LB nutrient agar tablets	5
Petri dishes, 60 mm, sterile	20
Inoculation loops, sterile	20
Disposable plastic transfer pipets	10
Module 2: Protein Purification
Ampicillin, lyophilized	1
Arabinose, lyophilized	1
Lysozyme, lyophilized	1
LB nutrient broth tablet	1
Binding buffer	1
Column equilibration buffer	1
Column wash buffer	1
Elution buffer	1
Chromatography columns	8
Inoculation loops, sterile	20
Disposable plastic transfer pipets	40
Micro test tubes, 2.0 ml, clear	30
Cell culture tubes, 15 ml, sterile	25
Sample collection tubes, 5 ml	25
Foam micro test tube holders	8
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide	1
Required Accessories Not Included in Kit:
UV lamps	4–8
Centrifuges	1–2
Recommended (Optional) Accessories:
Incubation oven
Rocking platform
Microwave oven

Secrets of the Rainforest Kit Flowchart

Key Kit Features

Grow a bacterial library
Clone a gene of interest in bacteria
Express a protein of interest in bacteria
Purify a protein of interest from bacteria
Take a protein to market
Complete in four 45 min lab sessions

Curriculum Fit

Creating a Bacterial Library and Cloning a Gene

How is a bacterial library created and a gene cloned? A bacterial library is constructed by chopping up the genome of the medicinal plant (the "parent") with a restriction enzyme. Restriction enzymes recognize specific double-stranded DNA sequences and cut each DNA strand, often creating "sticky ends" known as 3' or 5' overhangs. This generates a random assortment of DNA fragments. Some of these DNA fragments from the medicinal plant will contain the gene that encodes the green glowing protein with anticancer properties. When the same restriction enzyme is used to cut (digest) a plasmid (the "destination") it produces an opening in the plasmid with sticky ends that are complementary to the sticky ends of the medicinal plant DNA fragments. Next, the plant DNA fragments are inserted into plasmids by joining (ligating) their sticky ends and covalently bound together by a DNA ligase enzyme. The resulting plasmids contain random assortments of DNA fragments derived from the genome of the medicinal plant. The plasmids are transformed into the bacteria, E. coli. When transformed bacteria divide, the plasmid is also replicated. In the best-case scenario, some of the transformed bacterial cells will contain copies of the plasmid containing the plant gene, which encodes the protein of interest.

Secrets of the Rainforest colonies and GFP separation on a series of hydrophobic interaction chromatography (HIC) columns.

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The Secrets of the Rainforest™ Kit provides a biotechnology curriculum that takes students on an adventure starting in the rainforest in the Andes, continuing to a biotechnology company engaged in developing new pharmaceutical compounds, and finally to the Food and Drug Administration.

Features and Benefits

Applies the principles of DNA restriction analysis, bacterial transformation, and protein purification
Introduces your students to the world of commercial biotechnology

With this kit, students are able to:

Grow a bacterial library
Clone a gene of interest in bacteria
Express a protein of interest in bacteria
Purify a protein of interest from bacteria
Simulate taking a protein to market

How It Works

In the hands-on portion of the exercise, students streak out the bacterial library, grow the bacteria, and select the colonies that have acquired the green glowing trait from a background of nonglowing bacteria. A culture of bacteria is grown to scale up production of the green glowing protein, which is then purified by column chromatography. The actual source of this fluorescent protein is the bioluminescent jellyfish Aequorea victoria.

This practical activity is followed by dry-lab lessons in which the purified green protein is put through the drug discovery process. This includes learning about Food and Drug Administration regulations, animal testing, marketing practices for introducing a new drug, financing, ethical issues, and student presentations. Students examine the needs and viewpoints of advocacy groups, the biotechnology industry, and the FDA. They consider ethical, economic, social, and practical issues, and develop possible strategies for what they feel are the best outcomes.

More Information

Four-session laboratory activity, 45 min per session
Provides sufficient materials for eight student workstations, up to four students per workstation

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
Module 1: Preparing a Bacterial Library
Bacterial library, E. coli, lyophilized	1
LB nutrient agar tablets	5
Petri dishes, 60 mm, sterile	20
Inoculation loops, sterile	20
Disposable plastic transfer pipets	10
Module 2: Protein Purification
Ampicillin, lyophilized	1
Arabinose, lyophilized	1
Lysozyme, lyophilized	1
LB nutrient broth tablet	1
Binding buffer	1
Column equilibration buffer	1
Column wash buffer	1
Elution buffer	1
Chromatography columns	8
Inoculation loops, sterile	20
Disposable plastic transfer pipets	40
Micro test tubes, 2.0 ml, clear	30
Cell culture tubes, 15 ml, sterile	25
Sample collection tubes, 5 ml	25
Foam micro test tube holders	8
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide	1

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Secrets of the Rainforest Kit

1660006EDU
Bacterial library screening kit, classroom study kit, includes bacterial library and protein purification modules, curriculum, and more, for 32 students; education use only

0 1660006EDU /templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0006EDU_0921121040390.xml 1660006EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlbfo 2017-11-14 07:07:24 true true internet/product_SKU jthvlbfo templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0006EDU_0921121040390.xml LSE 2019-05-04 10:44:24 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972638657 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 10:44:24 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0006EDU_0921121040390.xml templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0006EDU_0921121040390.xml 1660006edu-secrets-rainforest-kit 1660006EDU Secrets of the Rainforest Kit Secrets of the Rainforest Kit Bacterial library screening kit, classroom study kit, includes bacterial library and protein purification modules, curriculum, and more, for 32 students; education use only Bacterial library screening kit, classroom study kit, includes bacterial library and protein purification modules, curriculum, and more, for 32 students; education use only Bacterial library screening kit, classroom study kit, includes bacterial library and protein purification modules, curriculum, and more, for 32 students; education use only /webroot/web/images/lse/products/pglo_gfp_kits/sku_thumb/global/166-0006EDU_tn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_view/global/166-0006EDU_view.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_feature/global/166-0006EDU_feature.jpg RepEx 166-0006EDU Bacterial library screening kit, classroom study kit, includes bacterial library and protein purification modules, curriculum, and more, for 32 students; education use only 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit N 0 1660006, 1660006EDU, secrets, of, the, rainforest, bacterial, library, screening, protein, purification, modules, curriculum, cloning, expression, science, education, products, classroom false $215.00 true $ %24172.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660006EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660006EDU&CartQuote=QT&quantity= 1711 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660006EDU&AddToDefaultWishList=true&quantity= 41106200 215.00 1660006EDU

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Bacterial Library

1660411EDU
Pkg of 1, E. coli library, for use with Secrets of the Rainforest kit (#166-0006EDU); education use only

1660411EDU /templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0411EDU_0921121040444.xml 0 1660411EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlbf6 2017-11-14 07:07:24 true true internet/product_SKU jthvlbf6 templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0411EDU_0921121040444.xml LSE 2019-05-04 10:44:20 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972638783 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 10:44:20 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0411EDU_0921121040444.xml templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0411EDU_0921121040444.xml 1660411edu-bacterial-library 1660411EDU Bacterial Library Bacterial Library Pkg of 1, E. coli library, for use with Secrets of the Rainforest kit (#166-0006EDU); education use only Pkg of 1, E. coli library, for use with Secrets of the Rainforest kit (#166-0006EDU); education use only Pkg of 1, E. coli library, for use with Secrets of the Rainforest kit (#166-0006EDU); education use only /webroot/web/images/lse/products/pglo_gfp_kits/sku_thumb/global/166-0411EDU_tn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_view/global/166-0411EDU_view.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_feature/global/166-0411EDU_feature.jpg RepEx 166-0411EDU Pkg of 1, E. coli library, for use with Secrets of the Rainforest kit (#166-0006EDU); education use only Bacterial Library, secrets of the Rainforest kit, E. coli library, classroom refill kit 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit N 0 ,VN <p>The Bacterial Library is for use with the Secrets of the Rainforest™ Kit (#166-0006EDU). The <em>E. coli</em> library is supplied lyophilized. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated 166-0411EDU_0921121040444_en.html /webroot/web/html/lse/products/SKUs/autopopulated/166-0411EDU_0921121040444_en.html false $33.75 true $ %2427.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660411EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660411EDU&CartQuote=QT&quantity= 1711 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660411EDU&AddToDefaultWishList=true&quantity= 12161500 33.75 1660411EDU

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Lyophilized Components Refill Pack

1660015EDU
Consumable reagents for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes ampicillin, arabinose, LB nutrient broth tablet, lysozyme; education use only

1660015EDU /templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0015EDU_0921121040371.xml 1 1660015EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlb9w 2017-11-14 07:07:24 true true internet/product_SKU jthvlb9w templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0015EDU_0921121040371.xml LSE 2019-05-04 10:44:59 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972638699 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 10:44:59 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0015EDU_0921121040371.xml templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0015EDU_0921121040371.xml 1660015edu-lyophilized-components-refill-pack 1660015EDU Lyophilized Components Refill Pack Lyophilized Components Refill Pack Consumable reagents for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes ampicillin, arabinose, LB nutrient broth tablet, lysozyme; education use only Consumable reagents for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes ampicillin, arabinose, LB nutrient broth tablet, lysozyme; education use only Consumable reagents for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes ampicillin, arabinose, LB nutrient broth tablet, lysozyme; education use only /webroot/web/images/lse/products/pglo_gfp_kits/sku_thumb/global/166-0015EDU_tn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_view/global/166-0015EDU_view.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_feature/global/166-0015EDU_feature.jpg Lyophilized Components Refill Pack #166-0015EDU RepEx Lyophilized Components Refill Pack #166-0015EDU Consumable reagents for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes ampicillin, arabinose, LB nutrient broth tablet, lysozyme; education use only 1660015, 1660015EDU, lyophilized, components, refill, pack, gfp, chromatography, secrets, of, the, rainforest, ampicillin, arabinose, lb, nutrient, broth, tablet, lysozyme, science, education, products, classroom 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit N 0 ,VN <p>The Lyophilized Components Refill Package contains consumable reagents for the Green Fluorescent Protein Chromatography Kit (#166-0005EDU) and the Secrets of the Rainforest™ Kit (#166-0006EDU). The package includes ampicillin, arabinose LB nutrient broth tablet, and lysozyme. This product is for education use only. </p> /webroot/web/html/lse/products/SKUs/autopopulated/ 166-0015EDU_0921121040371_en.html /webroot/web/html/lse/products/SKUs/autopopulated//166-0015EDU_0921121040371_en.html 1158 <b>Refill contains sufficient lyophilized reagents for 8 student workstations (2–4 students per workstation)</b>   1 0 2453 Ampicillin, lyophilized 1 1 0 1065 Arabinose, lyophilized 1 1 0 1076 Lysozyme, lyophilized 1 1 0 1075 LB nutrient broth tablet 1 1 0 false $81.25 true $ %2465.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660015EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660015EDU&CartQuote=QT&quantity= 1711 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660015EDU&AddToDefaultWishList=true&quantity= 41105300 81.25 1660015EDU

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GFP Chromatography Buffers Refill Pack

1660016EDU
Consumable buffers for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes binding, equilibration, wash, and elution buffers; education use only

1660016EDU /templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0016EDU_0921121040480.xml 3 1660016EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlbac 2017-11-14 07:07:24 true true internet/product_SKU jthvlbac templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0016EDU_0921121040480.xml LSE 2019-05-04 10:45:02 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972638741 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 10:45:02 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0016EDU_0921121040480.xml templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0016EDU_0921121040480.xml 1660016edu-gfp-chromatography-buffers-refill-pack 1660016EDU GFP Chromatography Buffers Refill Pack GFP Chromatography Buffers Refill Pack Consumable buffers for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes binding, equilibration, wash, and elution buffers; education use only Consumable buffers for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes binding, equilibration, wash, and elution buffers; education use only Consumable buffers for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes binding, equilibration, wash, and elution buffers; education use only /webroot/web/images/lse/products/pglo_gfp_kits/sku_thumb/global/166-0016EDU_tn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_view/global/166-0016EDU_view.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_feature/global/166-0016EDU_feature.jpg GFP Chromatography Buffers Refill Pack #166-0016EDU RepEx GFP Chromatography Buffers Refill Pack #166-0016EDU Consumable buffers for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes binding, equilibration, wash, and elution buffers; education use only 1660016, 1660016EDU, gfp, chromatography, buffers, refill, pack, consumables, replacement, buffer, secrets, of, the, rainforest, binding, equilibration, wash, elution, reagent, reagents, science, education, products, classroom 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit N 0 ,VN <p>Green Fluorescent Protein Chromatography Kit Lyophilized Components Refill Package contains consumable buffers for the Green Fluorescent Protein Chromatography Kit (#166-0005EDU) and the Secrets of the Rainforest™ Kit (#166-0006EDU). The package includes binding, equilibration, wash, and elution buffers. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 166-0016EDU_0921121040480_en.html /webroot/web/html/lse/products/SKUs/autopopulated//166-0016EDU_0921121040480_en.html 1155 <b>Refill contains sufficient buffers for 8 student workstations (2–4 students per workstation)</b>   1 0 1066 Binding buffer 1 1 0 1454 Column equilibration buffer 1 1 0 1461 Column wash buffer 1 1 0 1072 Elution buffer 1 1 0 false $73.75 true $ %2459.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660016EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660016EDU&CartQuote=QT&quantity= 1711 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660016EDU&AddToDefaultWishList=true&quantity= 41116100 73.75 1660016EDU

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HIC Chromatography Columns and Caps

1660413EDU
Pkg of 8, hydrophobic interaction chromatography column with cap, for use with Biotechnology Explorer classroom kits; education use only

1660413EDU /templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0413EDU_0921121040426.xml 2 1660413EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlba8 2017-11-14 07:07:24 true true internet/product_SKU jthvlba8 templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0413EDU_0921121040426.xml LSE 2019-05-04 10:45:01 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972638825 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 10:45:01 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0413EDU_0921121040426.xml templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0413EDU_0921121040426.xml 1660413edu-hic-chromatography-columns-caps 1660413EDU HIC Chromatography Columns and Caps HIC Chromatography Columns and Caps Pkg of 8, hydrophobic interaction chromatography column with cap, for use with Biotechnology Explorer classroom kits; education use only Pkg of 8, hydrophobic interaction chromatography column with cap, for use with Biotechnology Explorer classroom kits; education use only Pkg of 8, hydrophobic interaction chromatography column with cap, for use with Biotechnology Explorer classroom kits; education use only /webroot/web/images/lse/products/pglo_gfp_kits/sku_thumb/global/166-0413EDU_tn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_view/global/166-0413EDU_view.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_feature/global/166-0413EDU_feature.jpg HIC Chromatography Columns and Caps #166-0413EDU RepEx HIC Chromatography Columns and Caps #166-0413EDU Pkg of 8, hydrophobic interaction chromatography column with cap, for use with Biotechnology Explorer classroom kits; education use only 1660413, 1660413EDU, hic, chromatography, columns, and, caps, refill, hydrophobic, interaction, column, biotechnology, explorer, classroom, science, education, products 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit N 0 ,VN <p>Hydrophobic Interaction Chromatography Columns and Caps, package of eight, are for use with Biotechnology Explorer™ classroom kits. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 166-0413EDU_0921121040426_en.html /webroot/web/html/lse/products/SKUs/autopopulated//166-0413EDU_0921121040426_en.html 2728 HIC columns 8 1 0 false $52.50 true $ %2442.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660413EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660413EDU&CartQuote=QT&quantity= 1711 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660413EDU&AddToDefaultWishList=true&quantity= 12161500 52.50 1660413EDU

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Poly-Prep Columns

7311550EDU
Pkg of 50, conical 0.8 x 4 cm empty polypropylene column, 9 cm high, 2 ml bed volume, 10 ml of sample volume; education use only

7311550EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Chromatography/731-1550EDU_0921121032943.xml 4 7311550EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlbj8 2017-11-14 07:07:16 true true internet/product_SKU jthvlbj8 templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Chromatography/731-1550EDU_0921121032943.xml LSE 2019-05-04 11:13:50 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972093502 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 11:13:50 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Chromatography/731-1550EDU_0921121032943.xml templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Chromatography/731-1550EDU_0921121032943.xml 7311550edu-poly-prep-columns 7311550EDU Poly-Prep Columns Poly-Prep Columns Pkg of 50, conical 0.8 x 4 cm empty polypropylene column, 9 cm high, 2 ml bed volume, 10 ml of sample volume; education use only Pkg of 50, conical 0.8 x 4 cm empty polypropylene column, 9 cm high, 2 ml bed volume, 10 ml of sample volume; education use only Pkg of 50, conical 0.8 x 4 cm empty polypropylene column, 9 cm high, 2 ml bed volume, 10 ml of sample volume; education use only /webroot/web/images/lse/products/pglo_gfp_kits/sku_thumb/global/731-1550EDU_tn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_view/global/731-1550EDU_view.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_feature/global/731-1550EDU_feature.jpg Poly-Prep Columns #731-1550EDU RepEx Poly-Prep Columns #731-1550EDU Pkg of 50, conical 0.8 x 4 cm empty polypropylene column, 9 cm high, 2 ml bed volume, 10 ml of sample volume; education use only 7311550, 7311550EDU, poly-prep, columns, conical, empty, polypropylene, column, small-scale, gravity-flow, purification, science, education, products, classroom 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Equipment-and-Supplies/Chromatography N 0 ,VN <p>Poly-Prep<sup>®</sup> Columns are conical 0.8 x 4 cm empty polypropylene columns, 9 cm high, with a 2 ml bed volume and a sample volume of 10 ml. They are sold as a package of 50. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 731-1550EDU_0921121032943_en.html /webroot/web/html/lse/products/SKUs/autopopulated//731-1550EDU_0921121032943_en.html 2709 Poly-Prep<sup>®</sup> columns, empty, 0.8 x 4 cm 50 1 0 7317005 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/731-7005_0921120638974.xml 7311555 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/731-1555_0921120639354.xml 1560131 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0131_0921120658411.xml false $96.00 true $ %2476.80 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7311550EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7311550EDU&CartQuote=QT&quantity= 1712 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7311550EDU&AddToDefaultWishList=true&quantity= 41105300 96.00 7311550EDU

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Macro-Prep HIC Support

1560080EDU
100 ml, weakly hydrophobic methyl chromatography support; education use only

1560080EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Chromatography/156-0080EDU_0921121032560.xml 5 1560080EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlbfg 2017-11-14 07:07:15 true true internet/product_SKU jthvlbfg templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Chromatography/156-0080EDU_0921121032560.xml LSE 2019-05-04 11:13:25 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972093208 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 11:13:25 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Chromatography/156-0080EDU_0921121032560.xml templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Chromatography/156-0080EDU_0921121032560.xml 1560080edu-macro-prep-hic-support 1560080EDU Macro-Prep HIC Support Macro-Prep HIC Support 100 ml, weakly hydrophobic methyl chromatography support; education use only 100 ml, weakly hydrophobic methyl chromatography support; education use only 100 ml, weakly hydrophobic methyl chromatography support; education use only /webroot/web/images/lse/products/pglo_gfp_kits/sku_thumb/global/156-0080EDU_tn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_view/global/156-0080EDU_view.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_feature/global/156-0080EDU_feature.jpg Macro-Prep HIC Support #156-0080EDU RepEx Macro-Prep HIC Support #156-0080EDU 100 ml, weakly hydrophobic methyl chromatography support; education use only 1560080, 1560080EDU, macro-prep, methyl, hic, support, hydrophobic, interaction, chromatography, media, medium, resin, protein, separation, purification, 50um, 50, particle, size, science, education, products, classroom 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Equipment-and-Supplies/Chromatography N 0 ,VN <p>Macro-Prep<sup>®</sup> HIC Support, 100 ml, is a weakly hydrophobic methyl chromatography support. The product is for use with the Green Fluorescent Protein Chromatography Kit (#166-0005EDU) and the Secrets of the Rainforest™ Kit (#166-0006EDU). This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 156-0080EDU_0921121032560_en.html /webroot/web/html/lse/products/SKUs/autopopulated//156-0080EDU_0921121032560_en.html 2708 Macro-Prep<sup>®</sup> HIC support, 100 ml 1 1 0 false $444.00 true $ %24355.20 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560080EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560080EDU&CartQuote=QT&quantity= 1712 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560080EDU&AddToDefaultWishList=true&quantity= 12000000 444.00 1560080EDU

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Number	Description	Options
3051	Secrets of the Rain Forest Kit, Biotechnology Explorer, Quick Guide	Click to download
4006098	Instruction Manual, Secrets of the Rain Forest Kit, Biotechnology Explorer, Rev B	Please login to download this document

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