pGLO Bacterial Transformation Kit for General Biology

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Overview

Study core genetics concepts with a three-dimensional approach to the favorite pGLO bacterial transformation activity.

 
 
 
 
 
 
 
 
 
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Description

Description

The pGLO Bacterial Transformation Kit for General Biology is a three-dimensional approach to the classic transformation lab. Teach core genetics concepts including gene expression and gene regulation using this hands-on and student-driven experimental design experience.

Features and Benefits

  • Includes consumables and reagents for 12 laboratory workstations or 48 students
  • Enables students to design and analyze two gene expression experiments
  • Allows students to create and revise models to explain their observations
  • Activities designed for three to five 50-minute class periods

Kit Activity Overview

  • Observe fluorescent organisms and create models to explain observations
  • Learn about plasmids and bacterial transformation
  • Conduct a bacterial transformation experiment to collect data about the role of antibiotic selection markers
  • Design and conduct and experiment to switch on the GFP gene in transformed bacteria
  • Design a biosensor based on bacterial transformation to solve a real-world problem

Additional Teaching Resources for the pGLO Lab

Visit the pGLO Plasmid Map and Resources page for more information about how the pGLO plasmid system works.

pGLO Classroom Kit Comparison

  pGLO Bacterial Transformation Kit for General Biology pGLO Bacterial Transformation Kit pGLO Transformation and Inquiry Kit for AP Biology
Overview A highly scaffolded three-dimensional approach to bacterial transformation and gene expression; students design their own step-wise experiments and write and revise models and scientific explanations. A laboratory skills-based approach to bacterial transformation; students follow a robust protocol for a well-controlled experiment while analyzing the details of the technique. An inquiry-based approach to bacterial transformation through experimental optimization; students ask their own questions and conduct multiple experiments to refine the parameters of the technique.
Primary curriculum concepts Gene expression, gene regulation, antibiotic resistance, artificial selection, experimental design, laboratory skills
Workstations 12 8 8
Laboratory activities 2 1 5
Class periods
(50 min)
3–5 2 Flexible to fit a range of class periods
 
 
 
 
 
 
 
 
 

Specifications

Specifications

Kit contains sufficient materials for 12 student workstations (2–4 students per workstation)
Plasmid (pGLO), lyophilized
20 μg
E. coli strain HB101 K-12, lyophilized
1 vial
LB nutrient broth, sterile
10 ml
LB nutrient agar powder
20 g
Ampicillin, lyophilized
30 mg
Arabinose, lyophilized
600 mg
Sterile transformation solution (CaCl2)
15 ml
Petri dishes, 60 mm, sterile
40
Inoculation loops, sterile
80
Microcentrifuge tubes, 2.0 ml, sterile, color-coded
60
Disposable plastic transfer pipets
50
Foam micro test tube holders
8
UV pen light
1

Ordering

Ordering

items

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17006991EDU
Bacterial transformation kit designed for general biology classrooms; includes pGLO plasmid, nutrient broth/powder, petri dishes, UV pen light, transformation reagents, and consumables for 48 students; educational use only

Accessories

Accessories

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1660501EDU
120 V, compact incubator for growing bacteria, includes adjustable shelf, non-mercury thermometer, over-temperature protection, holds eighty 6.5 cm culture plates, CE approved; education use only
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1660610EDU

120 V, vortex mixer, 0–3,000 rpm, continuous or touch activation, includes general-purpose cup attachment, CE approved; education use only

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1660504EDU

120 V, 6 L water bath, ambient to 100°C, includes stainless steel tank and gable cover, over-temperature protection, non-mercury thermometer, CE approved; education use only

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1660562EDU
Stainless steel chamber and aluminum block offer rapid and even heating of up to 24 x 1.5 ml microcentrifuge tubes, CE approved; education use only
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(Discontinued) Long-Wave UV Lamp
1660500EDU
Pkg of 1, handheld UV lamp, output of 300–400 nm, for excitation of Green Fluorescent Protein (GFP), requires 4 AA batteries, CE-approved; education use only
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1660530EDU
Pkg of 1, pocket-sized disposable UV light to illuminate Green Fluorescent Protein (GFP); education use only

Refills

Refills

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1660555EDU
Consumable reagents for pGLO Bacterial Transformation Kits, includes pGLO Plasmid, E. coli, nutrient broth, transformation solution, ampicillin, arabinose; education use only
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(Discontinued) pGLO™ Plasmid
1660405EDU
20 μg, lyophilized DNA, plasmid encoding the Green Fluorescent Protein (GFP), for use with the pGLO Bacterial Transformation Kit (#166-0003EDU); education use only
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(Discontinued) Arabinose
1660406EDU
600 mg, lyophilized reagent, gene expression activator for use with the pGLO™ Bacterial Transformation Kit (#166-0003EDU); education use only
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(Discontinued) Ampicillin
1660407EDU
30 mg, lyophilized reagent, antibiotic for use with the pGLO™ Bacterial Transformation Kit (#166-0003EDU); education use only
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1660409EDU
15 ml, sterile CaCl2 solution, for use with the pGLO™ Bacterial Transformation Kit (#166-0003EDU); education use only
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1660473EDU
Pkg of 600, 6 colors; education use only

Documents

Documents

Number Description Options
10000134657
Instructor Guide, pGLO Bacterial Transformation Kit for General Biology
10000134658
Student Guide, pGLO Bacterial Transformation Kit for General Biology
7438
pGLO for General Biology TEKS Alignment
7437
pGLO for General Biology NGSS Alignment