pGLO Bacterial Transformation Kit for General Biology

Overview

Lab Preparation Checklist

Activity Flowchart

PowerPoint Presentation for Classroom Use

Study core genetics concepts with a three-dimensional approach to the favorite pGLO bacterial transformation activity.

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Description
Specifications
Ordering
Accessories
Refills
Documents

Description

The pGLO Bacterial Transformation Kit for General Biology is a three-dimensional approach to the classic transformation lab. Teach core genetics concepts including gene expression and gene regulation using this hands-on and student-driven experimental design experience.

Features and Benefits

Includes consumables and reagents for 12 laboratory workstations or 48 students
Enables students to design and analyze two gene expression experiments
Allows students to create and revise models to explain their observations
Activities designed for three to five 50-minute class periods

Kit Activity Overview

Observe fluorescent organisms and create models to explain observations
Learn about plasmids and bacterial transformation
Conduct a bacterial transformation experiment to collect data about the role of antibiotic selection markers
Design and conduct and experiment to switch on the GFP gene in transformed bacteria
Design a biosensor based on bacterial transformation to solve a real-world problem

Additional Teaching Resources for the pGLO Lab

Visit the pGLO Plasmid Map and Resources page for more information about how the pGLO plasmid system works.

pGLO Classroom Kit Comparison

	pGLO Bacterial Transformation Kit for General Biology	pGLO Bacterial Transformation Kit	pGLO Transformation and Inquiry Kit for AP Biology
Overview	A highly scaffolded three-dimensional approach to bacterial transformation and gene expression; students design their own step-wise experiments and write and revise models and scientific explanations.	A laboratory skills-based approach to bacterial transformation; students follow a robust protocol for a well-controlled experiment while analyzing the details of the technique.	An inquiry-based approach to bacterial transformation through experimental optimization; students ask their own questions and conduct multiple experiments to refine the parameters of the technique.
Primary curriculum concepts	Gene expression, gene regulation, antibiotic resistance, artificial selection, experimental design, laboratory skills
Workstations	12	8	8
Laboratory activities	2	1	5
Class periods (50 min)	3–5	2	Flexible to fit a range of class periods

Specifications

Kit contains sufficient materials for 12 student workstations (2–4 students per workstation)

Plasmid (pGLO), lyophilized

20 μg

E. coli strain HB101 K-12, lyophilized

1 vial

LB nutrient broth, sterile

10 ml

LB nutrient agar powder

20 g

Ampicillin, lyophilized

30 mg

Arabinose, lyophilized

600 mg

Sterile transformation solution (CaCl₂)

15 ml

Petri dishes, 60 mm, sterile

Inoculation loops, sterile

Microcentrifuge tubes, 2.0 ml, sterile, color-coded

Disposable plastic transfer pipets

Foam micro test tube holders

UV pen light

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(Discontinued) pGLO Bacterial Transformation Kit for General Biology

17006991EDU

Bacterial transformation kit designed for general biology classrooms; includes pGLO plasmid, nutrient broth/powder, petri dishes, UV pen light, transformation reagents, and consumables for 48 students; educational use only

Accessories

(Discontinued) Mini Incubation Oven, 120 V

1660501EDU

120 V, compact incubator for growing bacteria, includes adjustable shelf, non-mercury thermometer, over-temperature protection, holds eighty 6.5 cm culture plates, CE approved; education use only

(Discontinued) BR-2000 Vortexer, 120 V

1660610EDU

120 V, vortex mixer, 0–3,000 rpm, continuous or touch activation, includes general-purpose cup attachment, CE approved; education use only

(Discontinued) Temperature-Controlled Water Bath

1660504EDU

120 V, 6 L water bath, ambient to 100°C, includes stainless steel tank and gable cover, over-temperature protection, non-mercury thermometer, CE approved; education use only

(Discontinued) Digital Dry Bath, 120 V

1660562EDU

Stainless steel chamber and aluminum block offer rapid and even heating of up to 24 x 1.5 ml microcentrifuge tubes, CE approved; education use only

(Discontinued) Long-Wave UV Lamp

1660500EDU

Pkg of 1, handheld UV lamp, output of 300–400 nm, for excitation of Green Fluorescent Protein (GFP), requires 4 AA batteries, CE-approved; education use only

(Discontinued) Long-Wave UV Pen Light

1660530EDU

Pkg of 1, pocket-sized disposable UV light to illuminate Green Fluorescent Protein (GFP); education use only

Refills

(Discontinued) Transformation Reagent Refill Kit

1660555EDU

Consumable reagents for pGLO Bacterial Transformation Kits, includes pGLO Plasmid, E. coli, nutrient broth, transformation solution, ampicillin, arabinose; education use only

(Discontinued) LB Nutrient Agar Powder

1660472EDU

500 g, makes one thousand
60 mm agar plates; education use only

(Discontinued) Petri Dishes

1660470EDU

Pkg of 500, 60 mm x 15 mm, sterile; education use only

(Discontinued) Disposable Plastic Transfer Pipets

1660474EDU

Pkg of 500, sterile; education use only

(Discontinued) pGLO™ Plasmid

1660405EDU

20 μg, lyophilized DNA, plasmid encoding the Green Fluorescent Protein (GFP), for use with the pGLO Bacterial Transformation Kit (#166-0003EDU); education use only