pGLO Bacterial Transformation Kit for General Biology
Study core genetics concepts with a three-dimensional approach to the favorite pGLO bacterial transformation activity.
Overview
The pGLO Bacterial Transformation Kit for General Biology is a three-dimensional approach to the classic transformation lab. Teach core genetics concepts including gene expression and gene regulation using this hands-on and student-driven experimental design experience.
Features and Benefits
- Includes consumables and reagents for 12 laboratory workstations or 48 students
- Enables students to design and analyze two gene expression experiments
- Allows students to create and revise models to explain their observations
- Activities designed for three to five 50-minute class periods
Kit Activity Overview
- Observe fluorescent organisms and create models to explain observations
- Learn about plasmids and bacterial transformation
- Conduct a bacterial transformation experiment to collect data about the role of antibiotic selection markers
- Design and conduct and experiment to switch on the GFP gene in transformed bacteria
- Design a biosensor based on bacterial transformation to solve a real-world problem
Additional Teaching Resources for the pGLO Lab
Visit the pGLO Plasmid Map and Resources page for more information about how the pGLO plasmid system works.
PowerPoint Presentation Download an editable, student-facing presentation for classroom use.
| pGLO Bacterial Transformation Kit for General Biology | pGLO Bacterial Transformation Kit | pGLO Transformation and Inquiry Kit for AP Biology | |
|---|---|---|---|
| A highly scaffolded three-dimensional approach to bacterial transformation and gene expression; students design their own step-wise experiments and write and revise models and scientific explanations. | A laboratory skills-based approach to bacterial transformation; students follow a robust protocol for a well-controlled experiment while analyzing the details of the technique. | An inquiry-based approach to bacterial transformation through experimental optimization; students ask their own questions and conduct multiple experiments to refine the parameters of the technique. | |
| Primary curriculum concepts | Gene expression, gene regulation, antibiotic resistance, artificial selection, experimental design, laboratory skills | ||
| Workstations | 12 | 8 | 8 |
| Laboratory activities | 2 | 1 | 5 |
| Class periods(50 min) | 3–5 | 2 | Flexible to fit a range of class periods |
Specifications
Kit Contents
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Accessories
120 V, compact incubator for growing bacteria, includes adjustable shelf, non-mercury thermometer, over-temperature protection, holds eighty 6.5 cm culture plates, CE approved; education use only
120 V, vortex mixer, 0–3,000 rpm, continuous or touch activation, includes general-purpose cup attachment, CE approved; education use only
120 V, 6 L water bath, ambient to 100°C, includes stainless steel tank and gable cover, over-temperature protection, non-mercury thermometer, CE approved; education use only
Stainless steel chamber and aluminum block offer rapid and even heating of up to 24 x 1.5 ml microcentrifuge tubes, CE approved; education use only
Pkg of 1, pocket-sized disposable UV light to illuminate Green Fluorescent Protein (GFP); education use only
Refills
Consumable reagents for pGLO Bacterial Transformation Kits, includes pGLO Plasmid, E. coli, nutrient broth, transformation solution, ampicillin, arabinose; LB agar powder (1660600EDU) not included; for education use only
60 mm agar plates; education use only
