Immun-Blot® Opti-4CN™ Colorimetric Kits

Image
lsr_immun_blot_opti_4cn_colorimetric_kits.jpg

Overview

Bio-Rad's HRP-based colorimetric detection systems offer a choice of HRP-conjugated secondary antibodies and HRP-conjugated protein A or protein G using the detection reagent 4-chloro-1-naphthol II (4CN).

Read full description Ordering Information

Description

Description

Enzymes such as HRP or AP convert several substrates to a colored precipitate. As the precipitate accumulates on the blot, a colored signal develops. The reaction can be monitored and stopped when the desired signal over background is observed. Colorimetric detection is easier to perform than film-based chemiluminescence detection; however, the method's single endpoint result does not allow the multiple exposures of chemiluminescence methods. Colorimetric detection is typically considered a medium-sensitivity method compared to radioactive or chemiluminescence detection.

Bio-Rad's HRP-based colorimetric detection systems offer a choice of HRP-conjugated secondary antibodies and HRP-conjugated protein A or protein G using the detection reagent 4-chloro-1-naphthol(4CN). Bio-Rad offers three types of kits based on this chemistry for colorimetric HRP detection; individual reagents are also available, including 3,3'-diaminobenzidine (DAB), an alternative reagent.

Ordering

Ordering

items

Use the filters below to refine results!

170-8235_tn.jpg
1708235
Colorimetric detection (µg) kit, includes optimized 4CN substrate; for 2,500 cm2 of membrane (~50 mini blots)
170-8237_tn.jpg
1708237
Colorimetric detection (µg) kit, includes secondary antibody, optimized 4CN substrate; for 2,500 cm2 of membrane (~50 mini blots)
170-8236_tn.jpg
1708236
Colorimetric detection (μg) kit, includes secondary antibody, optimized 4CN substrate; for 2,500 cm2 of membrane (~50 mini blots)
170-8238_tn.jpg
1708238
High sensitivity (5 pg) colorimetric detection kit, includes optimized 4CN substrate, amplification reagents; for 2,500 cm2 of membrane (~50 mini blots)
170-8240_tn.jpg
1708240
High sensitivity (5 pg) colorimetric detection kit, includes optimized 4CN substrate, antibody conjugate, amplification reagents; for 2,500 cm2 of membrane (~50 mini blots)
170-8239_tn.jpg
1708239
High sensitivity (5 pg) colorimetric detection kit, includes optimized 4CN substrate, antibody conjugate, amplification reagents; for 2,500 cm2 of membrane (~50 mini blots)
170-6463_tn.jpg
1706463
Colorimetric immunodetection kit, includes 4CN, horseradish peroxidase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays
170-6464_tn.jpg
1706464
Colorimetric immunodetection kit, includes 4CN, horseradish peroxidase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays
170-6465_tn.jpg
1706465
Colorimetric immunodetection kit, includes 4CN, horseradish peroxidase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays
170-6431_tn.jpg
1706431
Colorimetric HRP substrate reagent kit, includes premixed 4CN, hydrogen peroxide solutions, color development buffer; makes 1 L color development solution
170-6534_tn.jpg
1706534
5 g, 4-chloro-1-naphthol (4CN), colorimetric blotting substrate; used with peroxidase antibody conjugate
170-6535_tn.jpg
1706535
5 g, 3,3'-diaminobenzidine (DAB), colorimetric blotting substrate; used with peroxidase antibody conjugate

Documents

Documents

Number Description Options
LIT178
Instruction Manual, HRP Conjugate Substrate Kit, Rev C
LIT387
Instruction Manual, HRP Color Development Solution, DAB (diaminobenzidine)
LIT320
Instruction Manual, HRP Color Development Solution 4CN (4-chloro-1-naphthol), Rev B
2032
Western Blotting Detection Reagents Brochure, Rev F
2260
Opti-4CN and Amplified Opti-4CN Protein Blotting Kits Flier, Rev A
4100130
Instruction Manual, Opti-4CN and Amplified Opti-4CN Kits, Rev C