Immun-Blot® AP Colorimetric Kits

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Immun-Blot AP Chemistry Colorimetric Kits

Overview



Specifications
Substrate Sensitivity Color Product Options
BCIP/NBT 100 pg Purple Dry powder, liquid substrate, Immun-Blot kits
Colorimetric Chemistry

Colorimetric detection. The secondary antibody, which binds the antibody specific for the protein of interest, is conjugated to an enzyme; its substrate is converted to a colored precipitate.

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Enzymes such as HRP or AP convert several substrates to a colored precipitate. As the precipitate accumulates on the blot, a colored signal develops. The reaction can be monitored and stopped when the desired signal over background is observed. Colorimetric detection is easier to perform than film-based chemiluminescence detection; however, the method's single endpoint result does not allow the multiple exposures of chemiluminescence methods. Colorimetric detection is typically considered a medium-sensitivity method compared to radioactive or chemiluminescence detection.

Bio-Rad's AP-based colorimetric kits utilize 5-bromo-4-chloro-3-indolyl phosphate/Nitroblue Tetrazolium (BCIP/NBT), which reacts with the alkaline phosphatase to form a color precipitate for detection. The Immun-Blot AP assay kit includes all necessary reagents for colorimetric detection and can detect up to 100 pg of protein.

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Immun-Blot Goat Anti-Rabbit IgG (H + L)-AP Assay Kit

1706460
Colorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays

0 1706460 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6460_0921120401672.xml 1706460 /default/main/bio-rad/WORKAREA/default 09/21/09 12:04 PM en_US jthvle8y 2017-11-14 07:08:34 true true internet/product_SKU jthvle8y templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6460_0921120401672.xml LSR 2019-05-04 19:24:03 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556001589756 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 19:24:03 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6460_0921120401672.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6460_0921120401672.xml 1706460-immun-blot-goat-anti-rabbit-igg-h-l-ap-assay-kit 1706460 Immun-Blot Goat Anti-Rabbit IgG (H + L)-AP Assay Kit Immun-Blot Goat Anti-Rabbit IgG (H + L)-AP Assay Kit Colorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays Colorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays Colorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-6460_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-6460_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/170-6460_feature.jpg RepEx 170-6460 Colorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays 09/21/09 12:04 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 false $323.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706460&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706460&CartQuote=QT&quantity= 1178 true true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706460&AddToDefaultWishList=true&quantity= 12352200 323.00 1706460
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Immun-Blot Goat Anti-Mouse IgG (H + L)-AP Assay Kit

1706461
Colorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays

1 1706461 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6461_0921120401775.xml 1706461 /default/main/bio-rad/WORKAREA/default 09/21/09 12:04 PM en_US jthvleb6 2017-11-14 07:08:34 true true internet/product_SKU jthvleb6 templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6461_0921120401775.xml LSR 2019-05-04 19:24:08 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556001589809 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 19:24:08 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6461_0921120401775.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6461_0921120401775.xml 1706461-immun-blot-goat-anti-mouse-igg-h-l-ap-assay-kit 1706461 Immun-Blot Goat Anti-Mouse IgG (H + L)-AP Assay Kit Immun-Blot Goat Anti-Mouse IgG (H + L)-AP Assay Kit Colorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays Colorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays Colorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-6461_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-6461_view .jpg RepEx 170-6461 Colorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays 09/21/09 12:04 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 ,VN / false $323.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706461&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706461&CartQuote=QT&quantity= 1178 true true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706461&AddToDefaultWishList=true&quantity= 12352200 323.00 1706461
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Hazardous  
Immun-Blot Goat Anti-Human IgG (H + L)-AP Assay Kit

1706462
Colorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays

This product is being discontinued soon. Please contact customer service for more information.
(Discontinued) Blotting-Grade Biotinylated AP Conjugate

1706403
1 ml, alkaline phosphatase (AP) secondary antibody conjugate

This product is no longer available. Please contact technical support for more information on replacement product.
Blotting-Grade Blocker

1706404
300 g, nonfat dry milk for western blotting applications

6 1706404 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6404_0921120401756.xml 1706404 09/21/09 12:04 PM en_US true internet/product_SKU 2019-05-04 18:49:32 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6404_0921120401756.xml /default/main/bio-rad/WORKAREA/default ja9sm559 2017-11-14 07:08:34 true ja9sm559 templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6404_0921120401756.xml LSR N /default/main/bio-rad en 12/31/37 11:59 PM 1556908428629 2019-05-04 18:49:32 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6404_0921120401756.xml 1706404-blotting-grade-blocker 1 1706404 Blotting-Grade Blocker Blotting-Grade Blocker 300 g, nonfat dry milk for western blotting applications 300 g, nonfat dry milk for western blotting applications 300 g, nonfat dry milk for western blotting applications /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-6404_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-6404_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/170-6404_feature.jpg Blotting-Grade Blocker preview ,VN RepEx 170-6404 300 g, nonfat dry milk for western blotting applications 170-6404, 1706404, blotting-grade, blocker, nonfat, dry, milk, blotting, grade, western, blot, nonspecific, binding, immunodetection 09/21/09 12:04 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Use blotting-grade blocker for consistently low backgrounds on western blots.</p> <h2 class="descHeader">Related Products</h2> <p>If using an avidin-biotin detection system, use Tris- Buffered Saline with 1% Casein (<a href="/en-us/sku/1610782-1x-tris-buffered-saline-tbs-with-1-casein?ID=1610782">1610782</a>) or Phosphate- Buffered Saline with 1% Casein (<a href="/en-us/sku/1610783-1x-phosphate-buffered-saline-pbs-with-1-casein?ID=1610783">1610783</a>).</p> <ul> <li><a href="/en-us/product/detergents-blocking-reagents-for-blotting">Detergents and Blocking Reagents for Blotting </a></li> <li><a href="/en-us/category/western-blotting-membranes-filter-paper?ID=ef19d64e-5909-450d-9d71-cc5e59555033">Western Blotting Membranes and Filter Paper</a></li> <li><a href="/en-us/category/transfer-buffers">Transfer Buffers</a></li> <li><a href="/en-us/category/immunodetection-reagents-kits?ID=534c009d-cd86-4b33-956c-674d5a6ae054">Immunodetection Reagents and Kits</a> including <a href="/en-us/product/clarity-clarity-max-western-ecl-blotting-substrates?ID=e08909eb-3369-42ec-a585-df189004268f">Clarity&trade; ECL Western Blotting Substrate</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1706404.html /webroot/web/html/lsr/products/SKUs/autopopulated/1706404.html false $50.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706404&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706404&CartQuote=QT&quantity= 1207 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706404&AddToDefaultWishList=true&quantity= 41116100 50.00 1706404
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AP Conjugate Substrate Kit

1706432
Colorimetric AP substrate reagent kit, includes premixed BCIP and NBT solutions, color development buffer; makes 1 L color development solution

7 1706432 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6432_0921120401813.xml 1706432 /default/main/bio-rad/WORKAREA/default 09/21/09 12:04 PM en_US jcrud036 2017-11-14 07:08:34 true true internet/product_SKU jcrud036 templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6432_0921120401813.xml LSR 2019-05-04 19:24:13 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556001589649 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 19:24:13 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6432_0921120401813.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6432_0921120401813.xml 1706432-ap-conjugate-substrate-kit 1706432 AP Conjugate Substrate Kit AP Conjugate Substrate Kit Colorimetric AP substrate reagent kit, includes premixed BCIP and NBT solutions, color development buffer; makes 1 L color development solution Colorimetric AP substrate reagent kit, includes premixed BCIP and NBT solutions, color development buffer; makes 1 L color development solution Colorimetric AP substrate reagent kit, includes premixed BCIP and NBT solutions, color development buffer; makes 1 L color development solution /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-6432_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-6432_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/170-6432_feature.jpg RepEx 170-6432 Colorimetric AP substrate reagent kit, includes premixed BCIP and NBT solutions, color development buffer; makes 1 L color development solution 09/21/09 12:04 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 170-6432, 1706432, ap, conjugate, substrate, western, blot, blotting, blots, westens, alkaline, phosphatase, colorimetric, bcip, nbt, solutions, color, development, buffer false $170.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706432&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706432&CartQuote=QT&quantity= 1178 true true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706432&AddToDefaultWishList=true&quantity= 41116100 170.00 1706432
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Hazardous  
(Discontinued) AP Color Development Reagent

1706539
300 mg, 5-bromo-4-chloro-3-indolyl phosphate (BCIP), colorimetric substrate, for purple color development; requires AP color development reagent (#170-6532)

This product is no longer available. Please contact technical support for more information on replacement product.
Number Description Options
2032 Western Blotting Detection Reagents Brochure, Rev F Click to download
LIT169 Instruction Manual, Alkaline Phosphatase Conjugate Substrate Kit, Rev E Click to download
LIT171 Instruction Manual, Immun-Blot® Kit Click to download

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