Immun-Blot® AP Colorimetric Kits

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Overview

AP-based colorimetric kits utilize 5-bromo-4-chloro-3-indolyl phosphate/Nitroblue Tetrazolium (BCIP/NBT), which reacts with AP to form a color precipitate. Can detect as little as 100 pg of protein.
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Description

Description

Enzymes such as HRP or AP convert several substrates to a colored precipitate. As the precipitate accumulates on the blot, a colored signal develops. The reaction can be monitored and stopped when the desired signal over background is observed. Colorimetric detection is easier to perform than film-based chemiluminescence detection; however, the method's single endpoint result does not allow the multiple exposures of chemiluminescence methods. Colorimetric detection is typically considered a medium-sensitivity method compared to radioactive or chemiluminescence detection.

Bio-Rad's AP-based colorimetric kits utilize 5-bromo-4-chloro-3-indolyl phosphate/Nitroblue Tetrazolium (BCIP/NBT), which reacts with the alkaline phosphatase to form a color precipitate for detection. The Immun-Blot AP assay kit includes all necessary reagents for colorimetric detection and can detect up to 100 pg of protein.

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170-6460_tn.jpg
1706460
Colorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays
170-6461_tn.jpg
1706461
Colorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays
170-6462_tn.jpg
1706462
Colorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays
blotting-grade-biotinylated-ap-conjugate-thumb-170-6403.jpg
1706403
1 ml, alkaline phosphatase (AP) secondary antibody conjugate
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1706404
300 g, nonfat dry milk for western blotting applications
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1706432
Colorimetric AP substrate reagent kit, includes premixed BCIP and NBT solutions, color development buffer; makes 1 L color development solution
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1706539
300 mg, 5-bromo-4-chloro-3-indolyl phosphate (BCIP), colorimetric substrate, for purple color development; requires AP color development reagent (#170-6532)

Documents

Documents

Number Description Options
2032
Western Blotting Detection Reagents Brochure, Rev F
LIT169
Instruction Manual, Alkaline Phosphatase Conjugate Substrate Kit, Rev E
LIT171
Instruction Manual, Immun-Blot® Kit