ChemiDoc XRS+ System

Image
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Overview

The ChemiDoc XRS+ Gel Imaging System is the fastest detection system for chemiluminescence, fluorescence, and colorimetric imaging. Controlled by Image Lab Image Capture and Analysis Software.

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Description

Description

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The ChemiDoc XRS+ System is based on CCD high-resolution, high-sensitivity detection technology and modular options to accommodate a wide range of samples and support multiple detection methods including fluorescence, colorimetry, densitometry, chemiluminescence, and chemifluorescence. The system is controlled by Image Lab software to optimize imager performance for fast, integrated, and automated image capture and analysis of various samples.

The system accommodates a wide array of samples, from large handcast polyacrylamide gels to small ReadyAgarose Gels and various blots. The system is an ideal accompaniment to PCR, purification, and electrophoresis systems, enabling image analysis and documentation of restriction digests, amplified nucleic acids, genetic fingerprinting, RFLPs, and protein purification and characterization.

Key benefits include:

  • Gel and blot imaging and analysis are quick and accurate
  • Automated, hands-off routines; no training is required
  • Save and recall all the steps in the workflow for repeatable and reproducible results
  • Optimize the system at setup for image data that is always accurate, reproducible, and free of imaging artifacts
  • Wide range of applications with special accessories to preserve sample integrity for downstream research while ensuring user safety
  • Comprehensive, automated quantitative analysis of protein and DNA samples in seconds
  • Customize and organize data in reports
  • Obtain publication-quality results quickly

The ChemiDoc XRS+ System optimizes reproducibility and reliability of experimental data, enabling quantitative comparisons between different experiments. Using proprietary algorithms, this imaging system is calibrated at setup to ensure:

  • Images are in focus at all times, regardless of zoom level or sample position
  • Appropriate flat fielding correction is automatically and consistently applied to image data for every application
  • Imaging artifacts are automatically corrected

The ChemiDoc XRS+ System offers fast and sensitive chemiluminescence detection in addition to gel and blot documentation of fluorescent and colorimetric samples. The system includes a supersensitive 16-bit CCD camera that is supercooled for detection of faint samples. The ChemiDoc XRS+ system can be used for imaging with a wide variety of applications that require high resolution and sensitivity (such as chemiluminescent western blots).

The ChemiDoc XRS+ System eliminates the need to use costly and unreliable X-ray film technologies while providing quantitative and reproducable data in seconds. The system features a signal accumulation mode (SAM), which guides a user through determining optimum exposure time and capturing a desired image of a chemiluminescent sample.

Specifications

Specifications

Applications
 
Chemiluminescence
Yes
Fluorescence*
Yes
Colorimetry/densitometry
Yes
Gel documentation
Yes
Hardware Specifications
 
Maximum sample size
up to 28 x 36 cm
Maximum image area
up to 26 x 35 cm
Maximum image area
(For standard UV-excited gels) 25 x 26 cm
Excitation source
Epi-white light and trans-UV (302 nm) are standard (optional 365 nm lamp available); optional trans-white conversion screen and XcitaBlue™ UV/blue conversion screen available
Illumination control
5 modes (trans-UV, epi-white, and no illumination for chemiluminescence are standard); trans white and XcitaBlue conversion screens are optional
Detector
Supercooled CCD
Pixel size (H x V) 
6.45 x 6.45 µm
Cooling system
Peltier
Camera cooling temperature
-30°C controlled
Filter selector
3 positions (2 for filters, 1 without filter, for chemiluminescence)
Emission filters
1 included (standard), 3 optional
Dynamic range
>4.0 orders of magnitude
Pixel density (gray levels)
65,535
Dynamic flat fielding
Application specific, for all applications
Instrument size (L x W x H)
36 x 60 x 96 cm
Instrument weight
32 kg
Operating Ranges
 
Operating voltage
110/115/230 V AC nominal
Operating temperature
10–28°C (21°C recommended)
Operating humidity
<70% noncondensing
Automation Capabilities
 
Workflow automated selection
Application driven, user selected or recalled by a protocol
Workflow automated execution
Controlled by a protocol via application-specific setup for image area, illumination source, filter, analysis, and reporting
Workflow reproducibility
100% repeatability via recallable protocols; from image capture to quantitative analysis and reports
Autofocus (patent pending)
Precalibrated focus for any zoom setting or sample height
Image flat fielding**
Dynamic; precalibrated and optimized per application
Autoexposure
2 user-defined modes (intense or faint bands)
*Use the optional XcitaBlue kit if performing SYBR® Safe DNA applications, because the UV to blue conversion screen allows you to visualize DNA samples while protecting against UV damage.

**U. S. patent 5,951,838.
 

Ordering

Ordering

items

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170-8265_tn.jpg
1708265
Gel imaging system, includes darkroom, UV transilluminator, epi-white illumination, camera, power supply, cables, Image Lab software compatible with Windows or Mac OS

Accessories

Accessories

170-8199_tn.jpg
1708299
Upgrade package for ChemiDoc XRS+ System includes focus calibration target, flat fielding disk, installation guide, and user manual
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1709690
Software, PC or Mac, for automated image capture, optimization, and 1-D data analysis, for use with Gel Doc™ EZ, Gel Doc XR+, ChemiDoc™ XRS+, ChemiDoc MP, and GS-900™ imaging systems
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1708026
Pkg of 1, calibration target for Gel Doc™ XR+, ChemiDoc™ XRS+, and ChemiDoc MP imaging systems with Image Lab™ software
1708279
Pkg of 1, UV safety shield to protect users from UV light exposure during band excision; for use with all ChemiDoc and GelDoc Imaging Systems from the universal hood series
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1708081
Filter, standard emission, 62 mm for Gel Doc™ XR+ and ChemiDoc™ XRS+ systems
170-8289_tn.jpg
1708289
Pkg of 1, conversion screen for viewing Coomassie, silver stain, and other colorimetric gel stains; for use with non touch–screen ChemiDoc™ MP, ChemiDoc™ XRS+, and Gel Doc™ XR+ Systems
170-8182_tn.jpg
1708182
Pkg of 1, conversion screen for viewing SYBR® Green, SYBR® Safe, GFP, and Flamingo™ Fluorescent Gel Stains, includes viewing goggles; for use with ChemiDoc™ XRS+, non touch–screen ChemiDoc MP, and Gel Doc™ XR+ Systems
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1708008
Pkg of 1, accessory for flat fielding
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1708089
Optional thermal printer, for use with Bio-Rad imaging systems
(Discontinued) Fluorescent Ruler
1703759
Pkg of 1, accessory, for use with ChemiDoc™ XRS+, ChemiDoc MP, and Gel Doc™ XR+ systems
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(Discontinued) Gel Cutter
1703760
Pkg of 1, accessory, for use with ChemiDoc and Gel Doc Imaging Systems
gel-alignment-templates-170-8184_thumb.jpg
1708184
Pkg of 3 gel templates, for use with ChemiDoc™ XRS+, ChemiDoc MP, and Gel Doc™ XR+ Systems, includes template holder
170-8185_tn.jpg
1708185
Pkg of 1, goggles for use with SYBR Safe dyes and Blue Trays for use with ChemiDoc and GelDoc Imaging Systems
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1707581

Pkg of 4, printer paper rolls for use with Mitsubishi Thermal Printer (#170-8089)

1708279
Pkg of 1, UV safety shield to protect users from UV light exposure during band excision; for use with all ChemiDoc and GelDoc Imaging Systems from the universal hood series
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1708097
Pkg of 6, 302 nm replacement UV lamps for all ChemiDoc™ and Gel Doc™ Imaging Systems
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(Discontinued) 365 nm UV Lamps
1706887
Pkg of 6, replacement lamp for Gel Doc™ XR, ChemiDoc™ XRS, and ChemiDoc MP systems

Documents

Documents

Number Description Options
6356
Traditional vs. Stain-Free Western Blotting
6253
Stain-Free Western Blotting Brochure
[ Add to Cart (Free) ]
7051
Imaging Systems Family Flier
[ Add to Cart (Free) ]
6427
Bio-Rad Stain-Free Western Blotting
[ Add to Cart (Free) ]
6390
General Stain-Free Western Blotting Protocol
[ Add to Cart (Free) ]
10040758
Instruction Manual, Image Lab™ Software: Imaging Stain-Free Gels and Blots, Rev B
6434
Western Blot Normalization Using Image Lab™ Software, Quick Start Guide
[ Add to Cart (Free) ]
10016116
Gel Alignment Template Kit
MI1708124
Instruction Manual, White Light Transilluminator, Rev A
10000076955
Gel Doc™ XR+ and ChemiDoc™ XRS+ Imaging Systems with Image Lab™ Software Instrument Guide
5837
ChemiDoc XRS+ Imaging System Brochure
[ Add to Cart (Free) ]
10016999
Molecular Imager ChemiDoc XRS+ Installation Guide
2907
ChemiDoc XRS System Flier, Rev B
10014084
Instruction Manual, GelDoc XR and ChemiDoc XRS, Rev A
2577
Instruction Manual, Molecular Imager Gel Doc XR and ChemiDoc XRS Systems Installation Guide, Rev B
5576
Molecular Weight Estimation and Quantitation of Protein Samples Using Precision Plus Protein WesternC Standards, the Immun-Star WesternC Chemiluminescent Detection Kit, and the Molecular Imager ChemiDoc XRS Imaging System, Rev C
2589
Not All Bits Are Created Equal
4000126-14
Instruction Manual, The Discovery Series: Quantity One 1-D Analysis Software, Rev A
4000177
Instruction Manual, IPD Multi-Color Imaging Target, Rev A
3097
Imaging and Analysis: Tools for Acquisition and Analysis of Protein Expression Data Brochure, Rev B
5840
Molecular Imager Gel Doc XR+ and ChemiDoc XRS+ Systems Product Information Sheet, Rev A
6148
Transfer of High Molecular Weight Proteins to Membranes: A Comparison of Transfer Efficiency Between Blotting Systems, Rev B