Fluorescent Protein Stains

Description

Description

Fluorescent protein gel staining, when combined with an appropriate imager, is a superior approach for protein analysis compared to Coomassie and silver staining. Fluorescent gel stains offer advantages such as broad linear range, higher sensitivity, and mass spectrometry compatibility. Bio-Rad offers three types of fluorescent protein gel stains, each optimized for your imager requirements:

Oriole™ fluorescent gel stain — An easy-to-use, fast, sensitive, and highly linear protein gel stain for visualization and quantitation of proteins in SDS-PAGE gels. The simple protocol requires a one-step staining process without prior fixing. Since destaining is not required, protein samples can be accurately visualized and quantitated in less than 2 hours. The excitation and emission characteristics of Oriole make it ideal for UV-based imagers.

Flamingo™ fluorescent gel stain — This 10x solution contains a novel dye that fluoresces when bound to denatured proteins. This easy-to-use gel stain can be used in both 1-D and 2-D electrophoretic applications and can detect down to 0.5 ng of protein. The staining procedure is a simple two-step protocol that can be completed in as little as 5 hours. The steps are not time sensitive and destaining is not required. Stained gels may be stored in the dark at 2–8°C for up to 6 months without significant loss of imaging sensitivity. Flamingo fluorescent gel stain is compatible with mass spectrometry. Gels stained with Flamingo fluorescent gel stain can also be visualized with a variety of fluorescence imaging systems, but it is particularly optimized for laser-based fluorescence scanners such as the Molecular Imager® PharosFX™ system.

SYPRO® Ruby protein gel stain — The most widely used gel stain for 2-D electrophoresis, ready-to-use SYPRO® Ruby protein gel stain is compatible with mass spectrometry and Edman-based sequencing applications. It can be visualized with UV or blue light transilluminators or laser scanners.

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1703126

200 ml, 1x solution, ready-to-use fluorescent gel stain

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1703125

1 L, 1x solution, ready-to-use fluorescent gel stain

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1703138

5 L, 1x solution, ready-to-use fluorescent gel stain

Accessories

Accessories

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(Discontinued) Storage Box
1653430
Pkg of 1, small box for storing gels, holds up to 4 gels on small staining trays
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(Discontinued) Storage Box
1653429
Pkg of 1, large box for storing gels, holds up to 4 gels on large staining trays
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1653401
100–240 V, small gel staining device, includes 13 trays, 12 Criterion tray attachments, shaking rack, solution tank, lid with motor, shaker control, gel clip

Documents

Documents

Number Description Options
5991
Oriole Fluorescent Gel Stain: A Novel Fluorescent Stain for a Simpler 2-D PAGE to MALDI Workflow, Rev A
2533
SYPRO® Ruby Protein Gel Stain Flier
5705
Sensitivity and Protein-to-Protein Consistency of Flamingo Fluorescent Gel Stain Compared to Other Fluorescent Stains, Rev A
5346
Flamingo Fluorescent Gel Stain Product Information Sheet, Rev A
10003321
Instruction Manual, Flamingo Fluorescent Gel Stain, Rev A
4006173
Instruction Manual, SYPRO® Ruby Protein Stains
5900
Oriole Fluorescent Gel Stain PIS, Rev A
5754
Comparison of SYPRO® Ruby and Flamingo Fluorescent Gel Stains With Respect to Compatibility With Mass Spectrometry
5921
Oriole Fluorescent Gel Stain: Characterization and Comparison with SYPRO® Ruby Gel Stain
6493
Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide
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