CFX Opus Real-Time PCR Systems

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Overview

Choose the CFX Opus Real-Time PCR System for the reliability you depend on from Bio-Rad with improved connectivity and performance for your modern lab.

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Description

Description

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OPUS-TOURCFX Opus System Tour

Choose the CFX Opus Real-time PCR System for the reliability you depend on from Bio-Rad with improved connectivity and performance for your modern lab.

The CFX Opus System is the next evolution in qPCR from Bio-Rad. With improved thermal performance and our proprietary, accurate optical shuttle system, your data will be more consistent than ever. A sleek, modern design includes a refreshed and easy-to-use interface with new connectivity options and features.

In addition to ethernet compatibility, the CFX Opus System can connect directly to Wi-Fi for completely wireless operation and data retrieval. The CFX Opus System is capable of stand-alone operation and direct data transfer to a USB device, or it can be connected to a computer.

With internet connectivity, the CFX Opus System can be programmed to send your data and notifications directly to your email or local networked drive, enabling easy access by collaborators or from other network-connected locations.

CFX Opus is available in three different block configurations: CFX Opus 96 for most general qPCR applications, CFX Opus 384 for larger throughput needs, and CFX Opus Deepwell with larger volumes up to 125 µl.

Features and Benefits

  • Quick system set up — easy installation and factory-calibrated optics
  • Our best thermal uniformity and accuracy to date
  • Offers Wi-Fi connectivity and BR.io cloud platform analysis tools
  • Sensitive modern touch screen — responsive even with double gloves
  • Updated interface is easier to use and more intuitive than ever
  • Uniform optical system free of regular calibration requirements
  • Audit and e-signature compatibility with CFX Maestro Security Edition Software
  • Available in 96-well (5-color detection) and 384-well (4-color detection) formats

Applications and Uses

  • Gene expression without requiring ROX
  • End-point analysis for sequence detection
  • Protein thermal shift assays
  • Assay optimization with thermal gradient

Related Products

CFX Maestro Software Works Seamlessly with PrimePCR Assays and Panels

The expert design and wet-lab validation of all PrimePCR Assays ensure optimal assay performance. The integration of CFX Maestro Software with PrimePCR Assays aids in the analysis and interpretation of experimental results.

  • Begin analyzing data in a single step by importing target, reference, and control information from a PrimePCR run file directly into CFX Maestro Software
  • Combine data from multiple plates into a gene study to rapidly screen large numbers of targets or samples
  • Use powerful data visualization tools, such as hierarchical clustering and color-matched expression levels, to identify individual targets or clusters to consider for further investigation

Service Plans and Validation Tools for CFX Systems, Thermal Cyclers, and Real-Time PCR Instruments

Specifications

Specifications

Thermal Cycler
Maximum ramp rate, °C/sec
CFX Opus 96 System: 5
CFX Opus 384 System: 2.5
CFX Opus Deepwell System: 2.5
Average ramp rate, °C/sec
CFX Opus 96 System: 3.3
CFX Opus 384 System: 2
CFX Opus Deepwell System: 2
Heating and cooling method
Peltier
Lid, °C
30–110
Temperature
Range, °C
4–100
Accuracy, °C
±0.2 of programmed target at 90°C
Uniformity, °C
CFX Opus 96 and 384 Systems:
±0.3 well-to-well after 10 sec of arrival at target
CFX Opus Deepwell System:
±0.4 well-to-well after 10 sec of arrival at target
Gradient
Operational range, °C
30–100
Programmable span, °C
1–24
Optical Detection
Excitation
CFX Opus 96 System: 6 filtered LEDs
CFX Opus 384 System: 5 filtered LEDs
CFX Opus Deepwell System: 6 filtered LEDs
Detection
CFX Opus 96 System: 6 filtered photodiodes
CFX Opus 384 System: 5 filtered photodiodes
CFX Opus Deepwell System: 6 filtered photodiodes
Range of excitation wavelengths, nm
CFX Opus 96 System: 450–684
CFX Opus 384 System: 450–650
CFX Opus Deepwell System: 450–684
Range of emission wavelengths, nm
CFX Opus 96 System: 510–730
CFX Opus 384 System: 515–690
CFX Opus Deepwell System: 510–730
Sensitivity
Detects 1 copy of target sequence in human genomic DNA
Dynamic range
10 orders of magnitude
Software
Operating systems
Windows 10
System
Sample capacity, wells
96-well and 384-well versions
Sample size, µl
CFX Opus 96 System: 1–50 (10–50 recommended)
CFX Opus 384 System: 1–30 (5–30 recommended)
CFX Opus Deepwell System: 10-125
Communication interface
USB 2.0
Electrical approvals
IEC, CE
Dimensions (W x D x H), cm/in
33x56x36 / 13x22x14
Weight, kg/lb
22/48
Touch Screen
Adjustable from 15°–22°
Embedded Operating System
Windows 10 IoT

Ordering

Ordering

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12011319

96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, cables

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12011452

384-well, 4-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 384 base unit, cables

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12013346

Wi-Fi antenna enables Wi-Fi connection for CFX Opus in North America and other specific countries.

Download

Download

CFX Opus 96, CFX Opus 384, CFX Opus Deepwell Real-Time PCR Detection Systems Software Update
CFX-OPUS-SW

Ensures optimal performance from your instrument. Required for CFX Maestro v2.3 update. Version 1.3.102.0920

Documents

Documents

Number Description Options
7180
CFX Real-Time PCR Systems Protein Thermal Shift Assay Protocol
7444
A Model qPCR System Comparability Study: CFX Touch and CFX Opus Real-Time PCR Platforms
7279
CFX Opus Real-Time PCR System Brochure
[ Add to Cart (Free) ]
3280
CFX Opus Deepwell Real-Time PCR System Flier
7280
CFX Opus Real-Time PCR System Research Flier
[ Add to Cart (Free) ]
3282
CFX Opus Deepwell Real-Time PCR System Brochure
[ Add to Cart (Free) ]
7281
CFX Opus Real-Time PCR System BioPharma Flier
[ Add to Cart (Free) ]
10000119983
CFX Opus Instrument Guide
10000126764
CFX Maestro Software User Guide
10000124722
CFX Opus 96 and CFX Opus 384 Real-Time PCR Systems Quick Start Guide
7299
CFX Opus 96 Real-Time PCR system Product Information Sheet
7300
CFX Opus 384 Real-Time PCR system Product Information Sheet
6090
Amplification Reagents and Plastics Brochure
[ Add to Cart (Free) ]