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System Comparison | Stain-Free Western Blotting Workflow | ChemiDoc MP Imaging System | Benefits | Image Lab Software | Resources | Applications & Technologies | Related Products |
ChemiDoc Imagers offer best-in-class performance with ease of use for visible light (RGB) and far red/near infrared (FR/NIR) fluorescence and chemiluminescence detection and all general gel documentation applications. Stain-free imaging enables immediate visualization of proteins without gel staining and instant verification of protein transfer to blots.
ChemiDoc System Comparison
 ChemiDoc MP |
ChemiDoc |
 ChemiDoc XRS+ |
|
|---|---|---|---|
| Features |
High-end imaging system for the best fluorescence and chemiluminescence detection
|
Chemiluminescence sensitivity matching X-ray film
|
Reliable, easy to use and good chemiluminescence sensitivity
|
| Specs | Ordering | Accessories | Specs | Ordering | Accessories | Specs | Ordering | Accessories | |
| Western Blot Sensitivity | Best | Best | Good |
| User Interface | 12" graphical touch screen with Image Lab Touch Software | 12" graphical touch screen with Image Lab Touch Software | Keypad entry or computer control with Image Lab Software |
| Multiplex Fluorescence Imaging | Yes | Upgradable | No |
| Documentation | Brochure User Guide | Brochure User Guide | Brochure User Guide |
ChemiDoc Recommendations by Application
Choose the best imaging system for your lab based on your most important applications.
Blot Detection
| Chemidoc System | ChemiDoc MP | ChemiDoc | ChemiDoc XRS+ | |||
| Multiplex Fluorescence | ✓ | Upgradable | Not applicable | |||
| Chemiluminescence | Best | Best | Good | |||
| SYPRO Ruby Protein Blot Stain* | ✓ | ✓ | Not applicable | |||
| Colorimetric Blots | ✓ | ✓ | ✓ | |||
| Stain-Free Blots** | ✓ | ✓ | ✓ | |||
Nucleic Acid Detection
| Chemidoc System | ChemiDoc MP | ChemiDoc | ChemiDoc XRS+ | |||
|---|---|---|---|---|---|---|
| Ethidium Bromide | ✓ | ✓ | ✓ | |||
| SYBR® Green I or SYBR® Safe Dye | ✓ | ✓ | ✓ | |||
| Fast Blast™ DNA Stain | ✓ | ✓ | ✓ | |||
Protein Staining, 1-D Gels
Protein Staining, 2-D Gels
* Optimal with low fluorescence PVDF membrane
** Requires Bio-Rad's stain-free gels
Stain-Free Western Blotting
Stain-free western blotting allows you to quickly check electrophoresis and blot transfer quality and obtain truly quantitative western blotting results, updating traditional blotting techniques with innovative tools.
Faster Results. Better Data.
PRODUCT SPOTLIGHTChemiDoc MP Imaging System: Imagine No Compromises
The ChemiDoc MP Imaging System is an instrument for imaging and analyzing gels and western blots. It is designed to address multiplex fluorescent western blotting, chemiluminescence detection, general gel documentation applications, and stain-free technology imaging needs.
Benefits of ChemiDoc Imaging Systems
Complete Capabilities: Superior Images
Stain-Free
Total Protein Normalization
Learn how to properly design a quantitative western blotting experiment and faithfully compare protein expression levels, without concern for changing or overexpressed HKP’s.
See Data »
Chemiluminescence
Replaces Film
Save money on consumables and reduce environmental waste without compromising on performance. Get the sensitivity of film and the convenience of digital documentation.
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Multiplex Fluorescence
3 Colors
Save sample, avoid the errors commonly associated with stripping, reprobing, and cutting blots and answer more complex biological questions by optimizing your experiments to fluorescence.
See Data »
Image Lab Software
The Chemidoc System features the intuitive Image Lab Touch Software onboard, with capabilities such as auto image capture, auto analysis, user preferences, and many other features, making gel imaging and analysis incredibly easy.
Image Lab Touch Software Demo
Explore how intuitive it is to acquire fluorescent and chemiluminescent gels and blots on the ChemiDoc systems. This easy-to-use software is perfect for multi-group use and comes installed on both the ChemiDoc and ChemiDoc MP.
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Image Lab Software Introduction
Learn the basics of Image Lab Software for ChemiDoc and Gel Doc Imaging Systems. Find out how to acquire, display, edit and analyze gel and blot images, and then display and export data. This step-by-step webinar is divided into chapters so that you can quickly find the information that you need.
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Using Software for Total Protein Normalization on Western Blots
Get an in-depth look at how to normalize your multiplex fluorescence western blots to total protein signal. This video covers manual adjustment of lanes and bands, correcting for blot background, and how to export data for publication or further analysis of your results.
Image Lab Touch Software Resources
Bio-Rad provides instructional videos for Image Lab Touch Software 2.4, covering the primary applications of the ChemiDoc MP Imaging System. Topics include, logging in, Smart Tray Technology, capturing your image, and exporting data.
Resources
Free 26-Lane Electrophoresis & Western Blotting Layout Post-It Pad
Plan, track, and never miss another step.
Request Yours Now
A Defined Methodology for Reliable Quantification of Western Blot Data
(PDF 602 KB)
Stain-Free Approach for Western Blotting
(PDF 885 KB)
Multiplex Fluorescent Blot Detection Troubleshooting Guide
(PDF 994 KB)
Request a Free Fluorophore Reference Poster
Determine the best fluorophore for maximum sensitivity and high signal-to-noise ratio in your next experiment using this poster.
Introducing the Western Blot Learning Center
Perfect your western blotting. Learn from the experts.
Applications and Technologies
Western Blotting Doctor
Protocols, video tutorials, and selection guides are available to help you at every step of your electrophoresis experiments.
Horizontal Nucleic Acid Electrophoresis
Learn about gel boxes, running buffers, agarose types, and other factors affecting resolution in DNA gel electrophoresis.
2-D Electrophoresis, Imaging, and Analysis
2-DE is an essential step in protein characterization, purification and profiling, and posttranslational modification studies.
Stain-Free Imaging Technology
Visualize proteins on gels and blots without staining and destaining steps, and use total protein normalization for quantitative western blots.
In-Cell Western Assays
In-Cell Western Assays are high throughput quantitative and qualitative immunofluorescence assays that utilizes Bio-Rad's ChemiDoc MP Imaging System and Image Lab Software.
Related Products
Gel Documentation Systems
Automated gel imaging and documentation for research laboratories and cGMP manufacturing environments.
Protein Gels
Find the right Bio-Rad protein gel for your application. Choose SDS-PAGE and native PAGE gels, convert to TGX Precast Gels, or choose specialized gel chemistries.
Image Lab Software
Image acquisition and analysis software for Bio-Rad Gel Doc, ChemiDoc, and GS-900 Systems. Capture and analyze digital image data from electrophoresis gels and blots.
Protein Ladders and Standards (Markers)
Prestained and unstained molecular weight standards for protein electrophoresis applications including SDS-PAGE, western blotting, 2-D PAGE, and isoelectric focusing (IEF).
Biologics Analysis Workflow
A four-step solution for biological product analysis, this integrated system enables expedited electrophoresis, simplified staining, easy analysis, and data reproducibility.
Antibodies
Find solutions for all your antibody needs: an extensive inventory of primary and secondary antibodies, controls, and reagents plus custom products including monoclonal generation in just 8 weeks.
Nucleic Acid Standards
Choose molecular rulers with evenly spaced banding patterns (DNA ladders), premixed EZ Load™ standards, and specialty products such as pulsed field standards.
Protein Stains
Stains to visualize and quantify protein bands on gels and blots: Coomassie stains, highly sensitive fluorescent stains, silver stains, negative stains, and total protein blot stains.