2-DE is an essential step in biomarker discovery workflow, whether your goal is protein characterization, purification and profiling, or posttranslational modification studies.
How can automation in electrophoresis enhance reliability in experiments assessing the purity and yield of proteins or evaluating the integrity of RNA?
Details on how biomarkers such as hormones, cytokines, chemokines, growth factors, and phosphoproteins are used to investigate physiological or pharmacological mechanisms.
Explore different aspects of genomics — from functional genomics and biomarker discovery to mutation analysis, gene expression, transfection, and epigenetics.
Various aspects of how to develop, optimize, and validate biomolecular interaction assays using surface plasmon resonance (SPR) technology.
Bio-Rad’s V3 Western Workflow™ facilitates speed and validation at each step of a western blotting experiment — from running gels to quantifying proteins.
Tips for isolation, maintenance, differentiation, transfection and analysis of stem cells.
Explore applications of chromatography: recombinant protein purification, monoclonal antibody discovery, viral contaminant removal, protein isolation, and more.
Protocols, video tutorials, and selection guides to help you at every step of your electrophoresis experiments.
Various aspects of how PCR works, how to choose the right PCR instrument, how to design, optimize, analyze, and troubleshoot PCR assays.
Learn more about western blotting techniques. Find step-by-step protocols and helpful tips on equipment, membranes, transfer conditions, and detection methods.
An array of solutions to optimize, carry out, and troubleshoot MIQE-compliant real-time PCR experiments.
Comparison of parameters, sensitivity, and data analysis using Bio-Rad imaging systems and software to determine which imaging system is ideal for your application.
Information on the digital PCR technology, and tips on planning droplet digital PCR experiments as well as on absolute quantification of targets using ddPCR can be found here.
Useful information from sample preparation to imaging of 2-D gels, along with protocols, video tutorials, demonstrations, and troubleshooting tips.
Information and tips on electroporation, viral transfection vector, lipofection, and biolistics transformation for planning and selecting the appropriate transfection method.
Gel boxes, running buffers, available agarose types, as well as factors affecting resolution of DNA fragments, to help with agarose gel electrophoresis of DNA.
Manual and automated cell counting methods and their application to gene expression studies are presented and discussed here.
How can large DNA fragments be separated using PFGE? How to prepare plugs, optimize sample resolution, analyze images, and troubleshoot PFGE gels? Explore.
Details on the experimental design, data collection, analysis, and validation of multiplex protein assays using the Bio-Plex® suspension array system.
The microfluidic technology used in Bio-Rad’s Experion™ system helps to automate protein and nucleic acid separation, detection, and analysis.
How does this label-free system perform analysis of biomolecular interactions? How do you choose the right sensor chip for your analysis? Find out.
Description of the different types of chromatography methods available and how they can be applied to guide you in your protein purification experiments.
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