Troubleshooting Western Blots with the Western Blot Doctor™

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Overview

The Western Blot Doctor is a self-help guide developed by
Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems. Comprehensive solutions and suggestions are provided to help solve your particular western blotting challenges.

Related Topics: Protein Blotting Methods, Protein Blotting Equipment (Cells and Power Supplies), Membranes and Blotting Papers, Transfer Conditions, Protein Blotting Detection and Imaging, Transfer Buffers

Good western blotting results begin with high-quality protein electrophoresis results. For detailed descriptions of the best practices for optimal protein separation by polyacrylamide gel electrophoresis, visit the Protein Electrophoresis section.

Download individual western blotting protocols in PDF format in the Protocols tab below.

To start troubleshooting your western blotting problems, choose the type of problem you are experiencing.

 

I am having trouble with:

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A blot with poor loading controls - Western Blot Doctor A blot with signal saturation - Western Blot Doctor A blot with air bubbles - Western Blot Doctor
A blot with a blotchy background - Western Blot Doctor A blot with non-specific bands - Western Blot Doctor A blot with weak signal - Western Blot Doctor
 

 

Related Content

Literature
Number Description Download
6517 ChemiDocâ„¢ Touch Imaging System Brochure, Rev A Click to download
6427 Bio-Rad V3 Western Workflow, Rev A Click to download
6349 Total Protein Normalization Publication List, Rev B Click to download
6350 Housekeeping Protein Normalization Publications, Rev A Click to download
6351 Stain-Free Technology Publications, Rev D Click to download
6356 Traditional vs. V3 Western Workflow Click to download
6360 A Method for Greater Reliability in Western Blot Loading Controls: Stain-Free Total Protein Quantitation, Rev B Click to download
RP0058 A Defined Methodology for Reliable Quantification of Western Blot Data Click to download
6359 Avoiding Housekeeping Protein Detection Saturation Protocol, Rev A Click to download
6361 Determining the Appropriate Film Exposure Time Protocol, Rev A Click to download
6362 Determining the Appropriate Sample Load for Western Blots Protocol, Rev A Click to download
6363 Determining the Appropriate Sample Load When Using a Stain-Free V3 Western Workflow™ Protocol, Rev A Click to download
6366 Validating the Expression Consistency of a Housekeeping Protein Protocol, Rev A Click to download
6376 General Protocol for Western Blotting Protocol, Rev A Click to download
6376 General V3 Western Workflow Blotting Protocol, Rev A Click to download
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