The Western Blot Doctor is a self-help guide that enables you to troubleshoot your western blotting problems. In this section, you can find solutions to problems with blot background signal.
Other sections in the Western Blot Doctor:
Click on the thumbnail that is most representative of your own blot to discover the probable causes and find specific solutions to the problem.
White spots or regions on membrane
High background across blot
Blotchy or patchy background
Uneven spots on blot or speckled background
Gel cassette shadow transferred to blot (tank blotters)
Foam pads for Bio-Rad wet tank blotting systems:
As a diagnostic test, you can check transfer quality by imaging proteins on the gel and blot. If planning to use the blot in downstream steps, make sure that your stain can be removed or is compatible with antibody detection. For example, Coomassie and colloidal gold are not compatible with downstream steps (see Bio-Rad Protein Stains and the Protein Stain Selection Guide).
Cooling for Bio-Rad Wet Tank Blotting Systems:
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