The Western Blot Doctor is a self-help guide that enables you to troubleshoot your western blotting problems. In this section, you can find solutions to issues related to protein band appearance.
Other sections in the Western Blot Doctor:
Click on the thumbnail that is most representative of your own blot to discover the probable causes and find specific solutions to the problem.
Inconsistent control protein levels among samples
Ghost protein bands
Swirls or missing bands; bands appear diffuse on blot
Bands are curved (smiling) not straight
Broad or misshapen bands
White (negative) bands on film using ECL method
Foam pads for Bio-Rad wet tank blotting systems:
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