Size Exclusion Chromatography Kit

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Overview

Column chromatography is the most common method used in biotechnology for separating a mixture of components in a liquid.This kit teaches the basic principles of size exclusion chromatography.
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Description

Description

The Size Exclusion Chromatography Kit teaches the basic principles of size exclusion chromatography, a type of column chromatography used to separate solubilized molecules by their size or molecular weight.

Features and Benefits

With this kit, students are able to:

  • Separate a mixture of colored biomolecules
  • Determine the number of components in a mixture
  • Study the chemical and physical properties of biomolecules
  • Apply the principles of chromatography

How It Works

In this laboratory activity, a mixture of colored molecules in solution, hemoglobin (reddish-brown; molecular mass of 65,000 Da) and vitamin B12 (pink; molecular mass of 1,350 Da), is applied to a chromatography column containing microscopic porous beads. The contrasting colors are easily visible as the molecules pass through the column. The larger molecules bypass and flow around the beads. They are excluded from entering the pores by their size and flow through the column with relative speed. Smaller molecules enter the porous beads and are impeded in their flow through the column. The solutes' different rates of travel through the column lead to effective separation.

More Information

  • One 45 min laboratory activity
  • Provides sufficient materials for eight student workstations, up to four students per workstation

Specifications

Specifications

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
 
Hemoglobin/vitamin B12 sample mixture
1
Bio-Gel® P-60 columns with caps
8
Disposable plastic transfer pipets
10
Column chromatography buffer, 50 ml
1
Sample collection tubes, 5 ml
100
Curriculum, including teacher's guide, student manual, and graphic quick guide
1

Ordering

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items

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1660008EDU
Basic column-chromatography classroom study kit, includes Bio-Gel P-60 columns, sample mixture, disposable pipets, buffer, collection tubes, curriculum, for 32 students; education use only

Refills

Refills

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1660018EDU
Refill reagent set, for use with size exclusion chromatography kit (#166-0008EDU), includes hemoglobin/vitamin B12 sample mixture, 50 ml buffer; education use only
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1660020EDU
Pkg of 8, size exclusion chromatography column with cap, for use with size exclusion chromatography kit; education use only
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(Discontinued) Bio-Gel P-60 Gel
1504160EDU
100 g, medium polyacrylamide beads for size exclusion and gel filtration chromatography; education use only
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(Discontinued) Poly-Prep Columns
7311550EDU
Pkg of 50, conical 0.8 x 4 cm empty polypropylene column, 9 cm high, 2 ml bed volume, 10 ml of sample volume; education use only

Documents

Documents

Number Description Options
3056
Size Exclusion Chromatography Kit, Biotechnology Explorer, Quick Guide
4006103
Instruction Manual, Size Exclusion Chromatography Kit, Biotechnology Explorer, Rev C