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Ready Gel Precast Gels for 2-D Electrophoresis

Ready Gel Precast Gels for 2-D Electrophoresis

The precast gels available for the Mini-PROTEAN Tetra and Mini-PROTEAN Dodeca cells are 8.6 x 6.8 cm Tris-HCl polyacrylamide gels in single-use cassettes.
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Overview

The precast gels available for the Mini-PROTEAN® Tetra and Mini-PROTEAN® Dodeca™ cells are 8.6 x 6.8 cm Tris-HCl polyacrylamide gels in single-use cassettes. This gel size provides reproducible, rapid results with fast setup, loading, and run times. For 2-D applications, the Ready Gel IPG combs are designed to hold 7 cm ReadyStrip IPG strips.

Ready Gel precast gels are packaged and sold in individual units: 1–4 gels can be run in the Mini-PROTEAN Tetra cell, and 1–12 gels can be run in the high-throughput Mini-PROTEAN Dodeca cell.

Gel Selection

Use the protein migration chart to select a gel percentage that will provide optimal resolution of your sample.

Use single-percentage gels if your sample contains a limited range of molecular weights, and choose the percentage in which the proteins of interest will migrate to the lower half of the gel.

Single-percentage gels provide the most effective resolution of proteins that are similar in molecular weight, and the best separation on single-percentage gels occurs in the lower half of the gel.

Use gradient gels if your sample contains a broad range of molecular weights. Gradient gels provide the most effective separation for samples across the range of molecular weights, and separation is optimized along the entire length of the gel. Molecules with a range of sizes can be separated on linear gradient gels because the larger pore sizes allow resolution of larger molecules, while the decreasing pore sizes toward the bottom of the gel restrict excessive separation of small molecules.

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(Discontinued) 8–16% Ready Gel® Tris-HCl Gel, 7 cm IPG/prep well, 250 µl
1611394
(Discontinued) 4–20% Ready Gel® Tris-HCl Gel, 7 cm IPG/prep well, 250 µl
1611393
(Discontinued) 4–15% Ready Gel® Tris-HCl Gel, 7 cm IPG/prep well, 250 µl
1611392
(Discontinued) 12% Ready Gel® Tris-HCl Gel, 7 cm IPG/prep well, 250 µl
1611391
(Discontinued) 10–20% Ready Gel® Tris-HCl Gel, 7 cm IPG/prep well, 250 µl
1611395
(Discontinued) 10% Ready Gel® Tris-HCl Gel, 7 cm IPG/prep well, 250 µl
1611390

Kits & Reagents

(Discontinued) 10x Tris/Glycine/SDS
1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
(Discontinued) 10x Tris/Glycine Buffer for Western Blots and Native Gels
1610734

Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water

(Discontinued) 10x Tris/Tricine/SDS Running Buffer, 1 L
1610744
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 100 mM Tris, 100 mM Tricine, 0.1% SDS, pH 8.3 following dilution to 1x with water
(Discontinued) 10x Tris/Glycine Buffer for Western Blots and Native Gels
1610771

Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water

(Discontinued) 10x Tris/Glycine/SDS
1610772
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
(Discontinued) Native Sample Buffer for Protein Gels, 30 ml
1610738
30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue

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