ProteOn Sensor and Maintenance Chips

Used with the ProteOn XPR36 protein interaction array system, ProteOn sensor chips combine advanced surface chemistries with state-of-the-art instrument microfluidics. Robust, real-time, label-free biomolecular interaction data are generated using this novel 6 × 6 surface plasmon resonance (SPR) technology.

Features and Benefits of ProteOn Sensor and Maintenance Chips

• Low cost per data point
• Range of surfaces designed for diverse protein interaction studies
• Easy optimization of capture surfaces using validated protocols
• Maintenance chip and protocols keep your system in excellent working order

Applications and Uses of Surface Plasmon Resonance Technology

• Large and small molecule screening
• High-throughput, label-free assay development and optimization
• Capture of lipid and lipid assemblies

ProteOn LCP Sensor Chip
The LCP sensor chip utilizes a NeutrAvidin-coated surface with a planar configuration. This SPR chip is designed for use with the ProteOn LCP reagent kit to capture both single and multiple layers of lipid assemblies such as liposomes.

ProteOn GLC Sensor Chip
The GLC sensor chip is designed for general amine coupling utilizing a compact polymer layer with a binding capacity of approximately one protein monolayer. This SPR chip is most suitable for protein-protein interaction analysis.

ProteOn GLM Sensor Chip
The GLM sensor chip is designed for general amine coupling and has an extended polymer matrix with intermediate binding capacity for high response. This SPR chip is suitable for both protein–small molecule and protein-protein interaction analyses.

ProteOn GLH Sensor Chip
The GLH sensor chip is designed for general amine coupling and has a highly extended polymer layer for maximum binding capacity. This SPR chip is most suitable for protein–small molecule applications in which high sensitivity is the primary objective.

ProteOn NLC Sensor Chip
The NLC sensor chip features NeutrAvidin immobilized on a GLC polymer layer with a binding capacity of approximately one protein monolayer. This SPR chip is an ideal choice for binding biotinylated proteins, peptides, and nucleic acids.

ProteOn HTG and HTE Sensor Chips
These ProteOn sensor chips feature novel tris-NTA complexes for improved capture of polyhistidine-tagged proteins. The complexes are attached to an alginate matrix layer and are activated by injection of Ni²⁺ ions and regenerated by injection of EDTA.

• ProteOn HTG sensor chip — has a lower tris-NTA surface density and less nonspecific binding, making it suitable for protein-protein and protein-peptide interaction analysis
• ProteOn HTE sensor chip — has an elevated tris-NTA surface density optimal for protein–small molecule interaction analysis

Each ProteOn sensor chip is enclosed in a bar-coded cartridge for reliable data tracking and archiving.

ProteOn Maintenance Chips
There are two maintenance sensor chips for use in the ProteOn XPR36 system. These chips are available individually or as part of the ProteOn maintenance kit:

• Maintenance (MNT) chip — used in maintenance protocols
• Cleaning (CLN) chip — used in the microfluidics cleaning protocol

The ProteOn XPR36 protein interaction analysis system includes all of the components required for interaction analysis: instrumentation, ProteOn Manager Software, ProteOn sensor chips, buffer and reagent kits, and protocol development kits.