ProteOn™ XPR36 Protein Interaction Array System

ProteOn™ XPR36 Protein Interaction Array System

Overview

The ProteOn™ XPR36 Protein Interaction Array System is an SPR optical biosensor that provides real-time data on the affinity, specificity, and interaction kinetics of protein interactions.
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Description

Description

Discontinued Product

The ProteOn XPR36 Instrument has been discontinued. All other ProteOn System products are still available for purchase. We will continue to provide support for ProteOn Systems until July, 2020.
Please contact Bio-Rad Technical Support for additional information.

The ProteOn™ XPR36 Protein Interaction Array System is an SPR optical biosensor that provides real-time data on the affinity, specificity, and interaction kinetics of protein interactions. Using XPR technology*, a unique approach to multiplexing, this system generates a 6 x 6 interaction array for the simultaneous analysis of up to six ligands with up to six analytes. The ProteOn XPR36 System increases the throughput, flexibility, and versatility of experiment design, enabling the completion of more experiments in less time:

  • Analyze up to 36 different protein interactions in a single run, on a single chip
  • Perform a complete kinetic analysis in a single run
  • Measure a variety of experimental conditions simultaneously in parallel
  • Screen multiple panels of analytes

See the ProteOn Technology and Applications videos for more information about how the ProteOn system works and how it can be applied to drug discovery and to address important experimental questions.

The ProteOn XPR36 protein interaction analysis system includes all of the components required for interaction analysis: instrumentation, ProteOn Manager™ Software, ProteOn Sensor Chips, buffer and reagent kits, and protocol development kits.

User-friendly ProteOn Manager Software uses a flexible, guided approach to coordinate instrument control, experiment setup, and data analysis.

Key Features and Benefits

  • Novel 6 x 6 array design
  • Real-time data acquisition
  • Analysis of interaction kinetics, binding affinity, and analyte concentration
  • No radiochemical or fluorescent labels needed
  • Integrated system

Applications and Uses

  • Antibody characterization and development
  • Large and small molecule drug development
  • Protein interface analysis
  • Protein complex and cascade analysis

*The ProteOn XPR36 System is covered by Bio-Rad patents, including United States patent numbers 8,111,400, 8,105,845, 7,999,942, and 7,443,507.

This product or portions thereof is manufactured and sold under license from GE Healthcare under United States patent numbers 5,554,541, 7,736,587, and 8,021,626, and any international patents and patent applications claiming priority.

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Specifications

Specifications

Number of interaction spots
36
Number of interspot references
42
Response uniformity
<2% CV
Refractive index range
1.33–1.37 refractive index units
Dynamic range, RU
1–40,000
Baseline noise
<1 RU, 1–20,000 RU
<1.5 RU, 20,000–40,000 RU
Baseline drift
<1 RU/min (15–40°C)*
Operating temperature range
15–40°C*
Autosampler temperature range
2–35°C
Sample configuration
72 x 1.5 ml vials or 2 x 96-well microplates
CCD
12-bit digital camera
Acquisition rate
3 Hz (3 images/sec), average 3 images/data point
Weight, kg
85
Dimensions (W x H x D), cm
95 x 58 x 50
Controller with 19" flat panel monitor (1,280 x 1,024 pixels) supplied with instrument
 
* Not lower than 15°C below room temperature.
 

Accessories

Accessories

176-6000_tn.jpg
(Discontinued) ProteOn Sample Rack
1766000
Pkg of 1, rack for 72 ProteOn sample vials (1.5 ml); for use with the ProteOn XPR36 protein interaction array system
176-6001_tn.jpg
(Discontinued) ProteOn Sample Rack Needle Arm
1766001
Pkg of 1, needle holder for rack autosampler layout and needle configuration in the ProteOn XPR36 system; accommodates ProteOn sample rack
176-6002_tn.jpg
(Discontinued) ProteOn Microplate Needle Arm
1766002
Pkg of 1, needle holder for microplate autosampler layout and needle configuration in the ProteOn system; accommodates standard and deep well microplates
176-6003_tn.jpg
(Discontinued) ProteOn Needles
1766003
Pkg of 6, needle, accommodates the ProteOn sample rack and ProteOn microplate needle holders
176-2700_tn.jpg
(Discontinued) ProteOn Running Buffer Bottle
1762700
Pkg of 1 bottle, holds 2 L running buffer
176-4114_tn.jpg
(Discontinued) ProteOn XPR36 Buffer Inlet Filters
1764114
Pkg of 2, buffer inlet filters for use with the ProteOn XPR36 protein interaction array system

Documents

Documents

Number Description Options
6550
Building New Anti-HIV Molecules with Bio-Rad’s ProteOn XPR36 System, Rev A
6132
The ProteOn HTG Sensor Chip: Novel Surface for Stable Capture of Histidine-Tagged Proteins for Protein-Protein Interaction Analysis, Rev A
6299
Guide to SPR Data Processing on the ProteOn XPR36 Protein Interaction Assay System, Rev A
6300
Guide to SPR Data Analysis on the ProteOn XPR36 System, Rev A
6295
ProteOn™ Sensor Chips, Tips and Techniques, Rev A
5948
Attachment of Membrane Protein Interactions Using Lipoparticles Bound to the Surface of the ProteOn XPR36 Sensor Chips, Rev A
5968
Screening and Characterization of Human Immunoglobulin G Binding Peptides Using the ProteOn XPR36 Protein Interaction Array System, Rev A
5960
High-Throughput Profiling of Kinase Inhibitors Selectivity Using the ProteOn™ XPR36 Protein Interaction Array System, Rev A
5390
ProteOn XPR36 Protein Interaction Array System Brochure, Rev D
5413
ProteOn XPR36 Hardware Product Information Sheet, Rev B
5627
ProteOn Manager Software Product Information Sheet, Rev C
5404
ProteOn™ Sensor Chips Product Information Sheet, Rev F
5410
ProteOn Protocol Development Kits Product Information Sheet, Rev B
5409
Protein Interaction Analysis Ordering Information Sheet, Rev E
5819
ProteOn XPR36 Protein Interaction Array System: Regulatory Tools for Drug Development Product Focus, Rev A
5965
Rapid, High-Throughput Screening of Protein Kinase Inhibitors Using the ProteOn XPR36 Protein Interaction Array System, Rev B
5846
Determining the Binding Kinetics of HIV-1 Nucleocapsid Protein to Six Densities of Oligonucleotide Using the ProteOn XPR36 Protein Interaction Array System, Rev A
5412
Antibody Characterization and Development: ProteOn XPR36 Protein Interaction Array System Application Guide, Rev C
5540
Screening, Ranking, and Epitope Mapping of Anti-Human IL-9 Supernatants, Rev A
5820
Rapid Screening and Selection of Optimal Antibody Capturing Agents Using the ProteOn XPR36 Protein Interaction Array System, Rev A
3172
Rapid and Efficient Determination of Kinetic Rate Constants Using the ProteOn XPR36 Protein Interaction Array System, Rev B
5360
Rapid and Detailed Analysis of Multiple Antigen-Antibody Pairs Using the ProteOn XPR36 Protein Interaction Array System, Rev A
5367
Rapid Optimization of Immobilization and Binding Conditions for Kinetic Analysis of Protein-Protein Interactions Using the ProteOn™ XPR36 Protein Interaction Array System, Rev A
5368
Anaylsis of Multiple Protein-Protein Interactions Using the ProteOn XPR36 Protein Interaction Array System, Rev B
5358
Mechanisms of Protein-Protein Binding: Double-Mutant Cycle Analysis Using the ProteOn XPR36 System, Rev A
5822
How to Perform Excluded Volume Correction on the ProteOn XPR36 Protein Interaction System Protocol Guide, Rev C
5797
Rapid Assay Development and Optimization for Small Molecule Drug Discovery, Rev B
5679
Applications of the ProteOn GLH Sensor Chip: Interactions Between Proteins and Small Molecules, Rev A
5449
Applications of the ProteOn NLC Sensor Chip: Antibody-Antigen, DNA-Protein, and Protein-Protein Interactions, Rev B
5434A
ProteOn XPR36 Protein Interaction Array System Folder. (Contains bulletins 3172, 5358, 5360, 5367, 5368, 5390, 5404, 5409, 5410, 5413, 5538, and 5465 DVD, depending on availability)
6161
Highly Efficient Lipoparticle Capture and SPR Binding Kinetics of a Membrane Protein Using the ProteOn XPR36 Protein Interaction Array System, Rev A
6254
The ProteOn™ HTE Sensor Chip: Novel Surface for Stable Capture of Histidine-Tagged Proteins for Protein-Small Molecule Interaction Analysis, Rev A
6449
Novel Liposome-Capture Surface Chemistries to Analyze Drug-Lipid Interaction using the ProteOn XPR36 System, Rev A
6414
ProteOn™ XPR36 Experimental Design and Application Guide, Rev B