Bio-Rad offers a variety of prepared solutions for casting polyacrylamide gels:
- Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). Use the 0.5 M Tris-HCl (pH 6.8) buffer for stacking gels and the 1.5 M Tris-HCl (pH 8.8) buffer for resolving gels
- Acrylamide solutions are provided ready to use and come with instructions. High-purity reagents and carefully controlled manufacturing conditions allow acrylamide solutions to be stable for 1 year at 4°C. Bio-Rad acrylamide solutions are available in two concentrations (30% and 40%) and in three acrylamide/bis ratios (19:1, 29:1, and 37.5:1)
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