Acrylamide Solutions and Powders

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Overview

Bio-Rad offers high purity acrylamide in three forms: acrylamide powders, premixed acrylamide/bis powders, and acrylamide solutions.

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Description

Description

Acrylamide solutions and powders are used to generate polyacrylamide, a chemically inert, electrically neutral, and hydrophilic gel matrix used for separating biomolecules by polyacrylamide gel electrophoresis (PAGE). The reliability of electrophoresis results depends on the reproducibility of this reaction, which is dependent on several factors.

The polymerization process requires bis-acrylamide as a cross-linking agent. Adjusting the concentration and ratio of acrylamide and bis-acrylamide creates gel matrices with a range of pore sizes that allow the electrophoretic separation of nucleic acids, peptides, and proteins — molecules ranging in molecular weight from 2 to 5,000 Da.

The purity of acrylamide reagents used in the polymerization process determines the uniformity of the gel matrix. Bio-Rad offers a range of 99.9% pure reagents to suit your DNA sequencing (6, 8, 12, 20% acrylamide), and/or peptide and protein electrophoresis applications. Our choices of pure acrylamide and/or bis-acrylamide premixed solutions or powders include:

Convenient ordering options are available to meet your acrylamide handcasting gel demands. Bio-Rad’s stock solutions and powders can be used to prepare

  • Single-percentage gels (7.5–20%) — for separating bands that are close in molecular weight
  • Gradient gels (4–15% and 10–20%) — for separating samples containing a broad range of molecular weights
  • Broad gradient gels (4–20% and 8–16%) — for evaluating new or unknown samples

More Information

Our accessories and reagents for handcasting multiple gels include polyacrylamide gel reagents, premade buffers and reagents, multicasting chambers for mini, midi, large gels, and gradient formers.

View our wide selection of mini and midi precast gels, electrophoresis equipment available in mini and midi formats, and protein standards for your specific protein separation applications.

Learn more about casting polyacrylamide gels, protein electrophoresis techniques, and types of protein electrophoresis (PAGE).

Ordering

Ordering

items

Use the filters below to refine results!

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1610154
500 ml, 30% acrylamide and bis-acrylamide solution, 19:1
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1610156
500 ml, 30% acrylamide and bis-acrylamide solution, 29:1
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1610158
500 ml, 30% acrylamide and bis-acrylamide solution, 37.5:1
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1610144
500 ml, 40% acrylamide and bis-acrylamide solution, 19:1
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1610146
500 ml, 40% acrylamide and bis-acrylamide solution, 29:1
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1610148
500 ml, 40% acrylamide and bis-acrylamide solution, 37.5:1
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1610140
500 ml, 40% acrylamide solution
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(Discontinued) 2% Bis Solution
1610142
500 ml, 2% bis-acrylamide solution
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1610155
2 x 500 ml, 30% acrylamide and bis-acrylamide solution, 19:1
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1610157
2 x 500 ml, 30% acrylamide and bis-acrylamide solution, 29:1
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1610159
2 x 500 ml, 30% acrylamide and bis-acrylamide solution, 37.5:1
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1610145
2 x 500 ml, 40% acrylamide and bis-acrylamide solution, 19:1
161-0147_tn.jpg
1610147
2 x 500 ml, 40% acrylamide and bis-acrylamide solution, 29:1
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1610149
2 x 500 ml, 40% acrylamide and bis-acrylamide solution, 37.5:1
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1610141
2 x 500 ml, 40% acrylamide solution
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(Discontinued) 2% Bis Solution
1610143
2 x 500 ml, 2% bis-acrylamide solution
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(Discontinued) Acrylamide
1610100
100 g, 99.9% pure acrylamide powder
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(Discontinued) Acrylamide
1610101
500 g, 99.9% pure acrylamide powder
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(Discontinued) Acrylamide
1610107
1 kg, 99.9% pure acrylamide powder
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(Discontinued) Acrylamide
1610103
2 kg, 99.9% pure acrylamide powder
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(Discontinued) Acrylamide
1610108
5 kg, 99.9% pure acrylamide powder
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1610120
30 g, 19:1 mixture of 99.9% pure acrylamide and bis-acrylamide powder
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1610121
30 g, 29:1 mixture of 99.9% pure acrylamide and bis-acrylamide powder
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1610122
30 g, 37.5:1 mixture of 99.9% pure acrylamide and bis-acrylamide powder
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1610123
150 g, 19:1 mixture of 99.9% pure acrylamide and bis-acrylamide powder
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1610124
150 g, 29:1 mixture of 99.9% pure acrylamide and bis-acrylamide powder
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1610125
150 g, 37.5:1 mixture of 99.9% pure acrylamide and bis-acrylamide powder

Kits & Reagents

Kits & Reagents

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1610170
Starter kit for hand casting 7.5% TGX polyacrylamide gels, includes acrylamide solutions and buffers
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1610171
Kit for hand casting 7.5% TGX polyacrylamide gels, includes acrylamide solutions and buffers
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1610172
Starter kit for hand casting 10% TGX polyacrylamide gels, includes acrylamide solutions and buffers
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1610173
Kit for hand casting 10% TGX polyacrylamide gels, includes acrylamide solutions and buffers
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1610174
Starter kit for hand casting 12% TGX polyacrylamide gels, includes acrylamide solutions and buffers
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1610175
Kit for hand casting 12% TGX polyacrylamide gels, includes acrylamide solutions and buffers
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1610180
Starter kit for hand casting 7.5% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers
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1610181
Kit for hand casting 7.5% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers
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1610182
Starter kit for hand casting 10% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers
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1610183
Kit for hand casting 10% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers
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1610184
Starter kit for hand casting 12% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers
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1610185
Kit for hand casting 12% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers

Documents

Documents

Number Description Options
6201
Handcasting Polyacrylamide Gels Protocol, Rev B
LIT306
Instruction Manual, Premixed, Preweighed Acrylamide:Bis, Rev C
1156
Acrylamide Polymerization — A Practical Approach
1866
Acrylamide Brochure
LIT492
Instruction Manual, Acrylamide and bis-Acrylamide Solutions
2317
Ready-to-Run Buffers and Solutions Brochure, Rev F