ELISA Immuno Explorer Kit

Image
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Overview

The kit utilizes a technique referred to as enzyme-linked immunosorbent assay (ELISA) to detect the presence of an antibody or an antigen in a sample.
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Description

Description

Note:This kit can be modified to accommodate at-home use as well as individual workstations in socially-distanced classrooms.
Download instructions for modification.

COVID-19 Hands-On Lesson Now Available

Teach the science behind SARS-CoV-2 antibody detection methods
using the ELISA Immuno Explorer Kit

Download the instructions and presentation

The ELISA Immuno Explorer Kit utilizes a technique referred to as enzyme-linked immunosorbent assay (ELISA) to detect the presence of an antibody or an antigen in a sample. The protocol uses two antibodies, one of which is specific to the antigen and another that is coupled to an enzyme. This second antibody gives the assay its enzyme-linked name and causes a chromogenic substrate to produce color wherever it is bound. ELISA is a powerful antibody-based biodetection tool used in the field to hunt for pathogens in water, food, or air, whether they emerge naturally or through acts of aggression.

Features and Benefits

  • Integrates multiple core content areas
  • Facilitates teaching about immune system functions and the unique properties of antibodies that have revolutionized medicine, epidemiology, and life science research

With this kit, students are able to:

  • Perform a genuine diagnostic procedure
  • Simulate real-world HIV, GMO, pregnancy, or drug testing

Applications and Uses

Three approaches to ELISA can be taken with this kit to teach either biodefense, immunology, or health science. Individual curriculum guides and instructions for each protocol are included in the kit, complete with Teachers Guides and Student Manuals.

More Information

  • One 45 min laboratory activity
  • Provides sufficient materials for twelve student workstations, up to four students per workstation

Additional Resources

Download this Zombie Apocalypse activity for a fun way to use the ELISA Immuno Explorer Kit in your classroom

Specifications

Specifications

For 12 workstations (2–4 students/workstation)
 
Antigen (chicken γ-globulin)*
1
Primary antibody (rabbit anti-chicken polyclonal antibody)*
1
Secondary antibody (goat anti-rabbit antibody, conjugated to horseradish peroxidase, or HRP)*
1
HRP enzyme substrate (TMB), 25 ml
1
10x PBS, 100 ml
1
10% Tween 20, 5 ml
1
Disposable plastic transfer pipets
80
Microplates with 12-well strips (8 rows of 12 wells)
3
Yellow micro test tubes, 2.0 ml
60
Colored micro test tubes, 2.0 ml
75
Curriculum, including teacher's guide, student manual, and graphic quick guide
1
*Convenient lyophilized reagents
 

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items

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166-2400EDU_tn.jpg
1662400EDU
Immunology classroom study kit, includes antigen, antibodies, HRP enzyme substrate, 10x PBS, 10% Tween, disposable plastic transfer pipets, test tubes, microplates, curriculum, for 48 students; education use only

Refills

Refills

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1662401EDU
Consumables refill reagents for use with ELISA Immuno Explorer™ Kit and the Giant Panda Problem Kit, contains antigen, antibodies, 10% Tween, 10x PBS, HRP enzyme substrate; education use only
166-2402_tn.jpg
1662402EDU
25 ml, HRP enzyme substrate (TMB); education use only
166-2408EDU_tn.jpg
1662408EDU
Antibody (goat anti-rabbit antibody conjugated to horseradish peroxidase [HRP]), lyophilized for shipping and long-term storage; education use only
166-2403EDU_tn.jpg
1662403EDU
100 ml, 10x Phosphate Buffered Saline (PBS); education use only
166-2404EDU_tn.jpg
(Discontinued) 10% Tween 20
1662404EDU
5 ml, 10% Tween 20; education use only
166-2405EDU_tn.jpg
1662405EDU
Pkg of 3, microplate, 8 rows of 12 wells each; education use only
166-2406EDU_tn.jpg
1662406EDU
25 μg, antigen (chicken-γ-globulin), lyophilized for shipping and long-term storage; education use only
166-2407EDU_tn.jpg
1662407EDU
25 μg, antibody (rabbit anti-chicken polyclonal), lyophilized for shipping and long-term storage; education use only
166-2408EDU_tn.jpg
1662408EDU
Antibody (goat anti-rabbit antibody conjugated to horseradish peroxidase [HRP]), lyophilized for shipping and long-term storage; education use only

Documents

Documents

Number Description Options
3136
ELISA Protocol III (Antibody Test), Biotechnology Explorer, Quick Guide, Rev A
3135
ELISA Protocol II (Antigen Detection), Biotechnology Explorer, Quick Guide, Rev B
3134
ELISA Protocol I (Tracking Disease Outbreaks), Biotechnology Explorer, Quick Guide, Rev B
4110175
Instruction Manual, ELISA Immuno Explorer Kit, Biotechnology Explorer, Rev E
6605
Map of AP-Aligned Bio-Rad Kits with Learning Objectives