ELISA Immuno Explorer Kit

Description

Description

Note:This kit can be modified to accommodate at-home use as well as individual workstations in socially-distanced classrooms.
Download instructions for modification.

COVID-19 Hands-On Lesson Now Available

Teach the science behind SARS-CoV-2 antibody detection methods
using the ELISA Immuno Explorer Kit

Download the instructions and presentation

Explore how antibodies are used in a real-world diagnostic assay

Use the ELISA Immuno Explorer Kit to teach your students how antibodies and the enzyme-linked immunosorbent assay (ELISA) are used to detect microbial and viral infections (such as those that cause COVID-19, HIV, or the flu), genetically modified organisms (GMOs), or molecular markers that enable diagnosis of cancer, pregnancy, or drug use.

This classroom lab activity connects multiple content areas, including the immune response, protein structure and function, and enzyme-substrate interactions, to illustrate how this use of antibodies has revolutionized medicine, epidemiology, agriculture, and life science research.

View our ELISA Guide to learn more about this technique.

Flexible Curriculum

The ELISA Immuno Explorer Kit allows you to choose one of several different activities to teach your students how protein structure and enzyme-substrate interactions help detect viruses (such as those that cause COVID-19, HIV, or the flu), genetically modified organisms (GMOs), or the molecular markers of cancer, pregnancy, or drug use.

Choose the test most relevant to your course:

  • Track a disease outbreak
  • Detect antigens (for example, virus, pregnancy hormone, drug, or disease detection)
  • Detect antibodies (past exposure to virus or other disease)

Optional lab activities not included in the instruction manual include:

Each activity can be completed within a single 45-min laboratory. To explore how ELISA is used to track levels of reproductive hormones in the context of species conservation efforts, see our Giant Panda Problem Kit for AP Biology.

ELISA Student Activities

ELISA Paper Model Activity

Your students can use this paper model activity alongside the videos or animations to get a solid grasp of the components of an ELISA and how they work together in antibody/antigen detection.

Download PDF (PDF 2.6 MB) | Download PPT (PPT 65.9 MB)

Additional Information

HOSA: future health professionals

This kit is a resource for the HOSA competitive event in Biotechnology. To learn more, visit HOSA.org or bio-rad.com/HOSA.

Specifications

Specifications

For 12 workstations (2–4 students/workstation)
 
Antigen (chicken γ-globulin)*
1
Primary antibody (rabbit anti-chicken polyclonal antibody)*
1
Secondary antibody (goat anti-rabbit antibody, conjugated to horseradish peroxidase, or HRP)*
1
HRP enzyme substrate (TMB), 25 ml
1
10x PBS, 100 ml
1
10% Tween 20, 5 ml
1
Disposable plastic transfer pipets
80
Microplates with 12-well strips (8 rows of 12 wells)
3
Yellow micro test tubes, 2.0 ml
60
Colored micro test tubes, 2.0 ml
75
Curriculum, including teacher's guide, student manual, and graphic quick guide
1
*Convenient lyophilized reagents
 

Ordering

Ordering

items

Use the filters below to refine results!

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166-2400EDU_tn.jpg
1662400EDU
Immunology classroom study kit, includes antigen, antibodies, HRP enzyme substrate, 10x PBS, 10% Tween, disposable plastic transfer pipets, test tubes, microplates, curriculum, for 48 students; education use only

Refills

Refills

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166-2401EDU_tn.jpg
1662401EDU
Consumables refill reagents for use with ELISA Immuno Explorer™ Kit and the Giant Panda Problem Kit, contains antigen, antibodies, 10% Tween, 10x PBS, HRP enzyme substrate; education use only
Image
166-2408EDU_tn.jpg
1662408EDU
Antibody (goat anti-rabbit antibody conjugated to horseradish peroxidase [HRP]), lyophilized for shipping and long-term storage; education use only
Image
166-2406EDU_tn.jpg
1662406EDU
25 μg, antigen (chicken-γ-globulin), lyophilized for shipping and long-term storage; education use only
Image
166-2407EDU_tn.jpg
1662407EDU
25 μg, antibody (rabbit anti-chicken polyclonal), lyophilized for shipping and long-term storage; education use only
Image
166-2408EDU_tn.jpg
1662408EDU
Antibody (goat anti-rabbit antibody conjugated to horseradish peroxidase [HRP]), lyophilized for shipping and long-term storage; education use only

Documents

Documents

Number Description Options
3136
ELISA Protocol III (Antibody Test), Biotechnology Explorer, Quick Guide, Rev A
3135
ELISA Protocol II (Antigen Detection), Biotechnology Explorer, Quick Guide, Rev B
3134
ELISA Protocol I (Tracking Disease Outbreaks), Biotechnology Explorer, Quick Guide, Rev B
4110175
Instruction Manual, ELISA Immuno Explorer Kit, Biotechnology Explorer, Rev E
6605
Map of AP-Aligned Bio-Rad Kits with Learning Objectives