Are you having trouble with your real-time PCR experiment? Do you need help diagnosing your experiment? The Real-Time PCR Doctor is here to help. Unexpected fluorescence data are symptomatic of problems with your real-time PCR reaction components or amplification protocol. Click one of the symptoms below to learn about possible causes and treatments.
Related Topics: What Is Real-Time PCR?, How Real-Time PCR Works, and qPCR Assay Design and Optimization.
Allelic Discrimination Experiments
Back to TopMy experiment displays:
 More than 3 clusters |
 Homozygotes as heterozygotes |
High Resolution Melt (HRM) Experiments:
Back to TopMy experiment displays:
 Missed call or clustering |
 Amplification did not reach plateau |
 Poor amplification or failed reaction |
If you don't see your symptom listed above, please contact technical support at 1-800-424-6723, option 2.
Further Reading
Hu CJ et al. (2006). Differential regulation of the transcriptional activities of hypoxia-inducible factor 1 alpha (HIF-1alpha) and HIF-2alpha in stem cells. Mol Cell Biol 26, 3514–3526. PMID: 16611993
Ivanova N et al. (2006). Dissecting self-renewal in stem cells with RNA interference. Nature 442, 533–538. PMID: 16767105
Li Y et al. (2006). DAPK1 variants are associated with Alzheimer's disease and allele-specific expression. Hum Mol Genet 15, 2560–2568. PMID: 16847012
Morozumi M et al. (2006). Simultaneous detection of pathogens in clinical samples from patients with community-acquired pneumonia by real-time PCR with pathogen-specific molecular beacon probes. J Clin Microbiol. 44, 1440–1446. PMID: 16597874
Ridd K et al. (2006). Association of gene expression with sequential proliferation, differentiation, and tumor formation in murine skin. Carcinogenesis 27, 1556–1566. PMID: 16537558
