Profinity™ IMAC Resin

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Profinity IMAC Resins

Overview

Affinity Chromatography Resin Selection Guide
  Matrix Functional Group Specificity Capacity Working pH Pressure Limit Applications
Ready-to-Use Affinity Media
Nuvia™ IMAC High capacity, pressure-stable polymer based on UNOsphere™ beads NTA charged with Ni2+ Histidine ≥40 mg/ml* 2–14 45 psi
(3.1 bar)
Purification of recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity™ IMAC Pressure-stable polymer based on UNOsphere beads IDA, available charged with Ni2+ and uncharged Histidine ≥15 mg/ml* 1–14 100 psi
(6.8 bar)
Suitable for purification of larger and difficult-to-purify recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity GST** Pressure-stable polymer based on UNOsphere beads Immobilized glutathione GST-tagged proteins ≥10 mg/ml 1–14 45 psi
(3.1 bar)
Purification of recombinant GST-tagged proteins
Profinity eXact™*** Crosslinked 6% agarose Subtilisin protease Subtilisin prodomain ≥3 mg/ml tag-free protein 2–10 10 psi (minus system pressure) Generation of native, tag-free proteins by on-column purification and cleavage
Affi-Gel® Protein A Crosslinked agarose Protein A
2 mg/ml
IgG   2–10 15 psi
(1 bar)
Purification of IgG from ascites, serum, and culture fluid; with MAPS buffer system, purification of 10 mg mouse IgG per ml of gel is possible
Affi-Prep® Protein A Pressure-stable polymer Protein A
2 mg/ml
IgG   2–10 1,000 psi (70 bar) Purified IgG from ascites, serum, and culture fluid; pressure-stable support for process-scale applications
Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA 1.9 mg/ml Albumin; general ≥11 mg/ml 2–10 15 psi
(1 bar)
Binds many nucleotide-requiring enzymes, albumin, and other proteins
DEAE Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and DEAE Albumin and serum proteins 0.14 ml serum/ml gel 2–10 15 psi
(1 bar)
Purifies protease-free IgG from ascites, serum, and culture fluid with minimal sample preparation
CM Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and CM Albumin and serum proteins 0.17–0.5 ml serum/ml gel 2–11 15 psi
(1 bar)
Produces albumin- and protease-free antibody preparation from serum without prior dialysis
Affi-Prep Polymyxin Pressure-stable polymer Polymyxin 2–4 mg/ml Endotoxins >5 mg/ml 2–10 1,000 psi
(70 bar)
Endotoxin removal
Affi-Gel Boronate Polyacrylamide gel Boronate 1.05 ± 0.15 cis-diols

meq/g
130 µmol sorbitol/ml 2–10 15 psi
(1 bar)
Adsorption of cis-hydroxyl–containing molecules, including sugars, nucleotides, and glycopeptides
Activated Media for Spontaneous Ligand Immobilization
Profinity Epoxide Pressure-stable polymer based on UNOsphere beads Epoxy group Nucleophiles; amini, thiol, -COOH 36–40 mg/ml lgG 1–14 Up to 80 psi
(5.5 bar)
Activated matrix for the immobilization of various ligands (for example, protein A, StrepTactin, and immunoglobulins)
Affi-Gel 10 Crosslinked agarose N-hydroxy-succinimide ≥10 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)
For coupling proteins with pI 6.5–11
Affi-Gel 15 Crosslinked agarose N-hydroxy-succinimide ≥9 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)
For coupling proteins with pI 6.5–11
Affi-Gel Hz Crosslinked agarose Hydrazide Oxidized carbohydrates 1–5 mg/ml 2–10 15 psi
(1 bar)
Immobilization of immunoglobulins and other glycoproteins via carbohydrate molecules
Affinity Media Using Carbodiimide Activation
Affi-Gel 102 Crosslinked agarose -NH2 16 ± 4 meq/ml -COOH 40 mg 2–11 15 psi
(1 bar)
Carbodiimide coupling of carboxyl-containing ligand

* Refer to Bulletin 3193 for purification conditions.
** For example, Profinity GST Resin is available only in prepacked cartridges.
***Profinity eXact Purification Resin.

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Profinity IMAC resin is an affinity chromatography support for the purification of recombinant histidine (His)-tagged proteins. Immobilized metal affinity chromatography (IMAC) is a powerful purification technique that relies on the affinity that His has for immobilized transition metals. Profinity IMAC resin, based on UNOsphere beads, contains iminodiacetic acid (IDA) as the chelating ligand for di- or trivalent metal ions. Its chemical structure allows highly selective binding of recombinant His-tagged proteins when charged with Ni2+ or other transition metals, such as Zn2+ or Cu2+.

Profinity IMAC offers high capacity at high flow rates. It can be used under either nondenaturing or denaturing conditions, and is suitable for purification using liquid chromatographic instrumentation, gravity-flow columns, or spin columns.

The resin is available in two forms: uncharged and precharged with Ni2+. The uncharged form can be charged with the metal ion of your choice for even greater purification flexibility.

Features of Profinity IMAC resins include:

  • Optimal ligand density for higher purity of target protein
  • Superb mechanical strength
  • Excellent pressure-flow properties for high maximum operating pressures and high flow rates, which allow rapid purification as well as rapid column cleaning and re-equilibration
  • Stable from pH 1 to 14
  • Compatible with denaturing agents, detergents, and reducing agents
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Profinity™ IMAC Resin, Ni-charged, 10 ml

1560131
Pkg of 1, 10 ml, ready-to-use support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography

0 1560131 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0131_0921120658411.xml 1560131 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jao0teoujao0teov" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jao0teoujao0teov"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="13" Second="20" ZoneOffsetMillis="0" Time="1512155600373">Fri Dec 01 19:13:20 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0131_0921120658411.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="13" Second="20" ZoneOffsetMillis="0" Time="1512155600373">Fri Dec 01 19:13:20 UTC 2017</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jao0temg 2017-11-14 07:08:02 true true internet/product_SKU LSR N en 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-01 00:17:45 1560131-profinity-imac-resin-ni-charged-10-ml 1560131 Profinity IMAC Ni-Charged Resin Profinity™ IMAC Resin, Ni-charged, 10 ml Pkg of 1, 10 ml, ready-to-use support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography 10 ml, ready-to-use support for immobilized metal affinity chromatography Pkg of 1, 10 ml, ready-to-use support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography /webroot/web/images/lsr/products/chromatography/sku_thumb/global/156-0131_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/156-0131_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/156-0131_feature.jpg preview RepEx 156-0131 10 ml, ready-to-use support for immobilized metal affinity chromatography 1560131, profinity, imac, nickel-charged, ni-charged, ni, nickel, charged, resin, immobilized, metal, affinity, chromatography, ready-to-use, support, media, hisitidine, his, tagged, his-tagged, protein, puritifcation, ml 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 <p>Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights.</p> <ul> <li>Chelating ligand is iminodiacetic acid (IDA)</li> <li>Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems</li> <li>Stable across a wide pH range (1&ndash;14)</li> <li> Works under native or denaturing conditions</li> <li>Can be used for single-step purification from crude samples or contaminant removal</li> </ul> <p>Profinity IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.</p> <h2 class="descHeader">Product Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="325" align="left" valign="top"><strong>Resin Form</strong></td> <td width="175" align="center" valign="top"><strong>Package Size </strong></td> <td width="319" align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top">1560131</td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">25 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560133-profinity-imac-resin-ni-charged-25-ml?ID=1560133">1560133</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">100 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560135-profinity-imac-resin-ni-charged-100-ml?ID=1560135">1560135 </a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560137-profinity-imac-resin-ni-charged-500-ml?ID=1560137">1560137 </a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560121-profinity-imac-resin-uncharged-10-ml?ID=1560121">1560121 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">50 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560123-profinity-imac-resin-uncharged-50-ml?ID=1560123">1560123</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560125-profinity-imac-resin-uncharged-500-ml?ID=1560125">1560125 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">1 L</td> <td align="center" valign="top"><a href=" /en-us/sku/1560127-profinity-imac-uncharged-resin">1560127</a></td> </tr> </tbody> </table> <p>Prepacked <a href="/en-us/product/bio-scale-mini-profinity-imac-cartridges">Bio-Scale&trade; Mini Profinity&trade; IMAC Cartridges</a> with uncharged or Ni-charged IDA, and <a href="/en-us/product/bio-scale-mini-nuvia-imac-cartridges">Bio-Scale&trade; Mini Nuvia&trade; IMAC Cartridges</a> with Ni-charged NTA are available.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Native IMAC Buffer Kit (<a href="/en-us/sku/6200239-native-imac-buffer-kit">6200239</a>) includes IMAC lysis, wash, and elution buffers</li> <li>Native IMAC Purification Kits, 1 ml (<a href="/en-us/sku/6200241-native-imac-purification-kit-1-ml">6200241</a>) includes Native IMAC buffer Kit and 2x1 ml Bio-Scale Mini IMAC Cartridges, and 5 ml (<a href="/en-us/sku/6200242-native-imac-purification-kit-5-ml">6200242</a>) includes Native IMAC buffer Kit and 1x5 ml Bio-Scale Mini IMAC Cartridge </li> <li><a href="/en-us/product/nuvia-imac-resin">Nuvia IMAC Resin</a>, Ni-charged nitrilotriacetic acid (NTA)</li> <li>Empty Bio-Scale Mini Cartridges, 1 ml (<a href="/en-us/sku/7324660-1-ml-empty-bio-scale-mini-cartridges">7324660</a>) and 5 ml (<a href="/en-us/sku/7324661-5-ml-empty-bio-scale-mini-cartridges">7324661</a>)</li> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>&reg;</sup> </a><a href="/en-us/product/poly-prep-chromatography-columns">Chromatography</a><a href="/en-us/product/poly-prep-chromatography-columns"> Columns</a>, hold up to 2 ml support</li> <li>His Antibody (<a href="/en-us/sku/6200203-his-antibody">6200203</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/ngc-medium-pressure-liquid-chromatography-systems?ID=MFCV7415">NGC&trade; Medium-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic&trade; Low-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/profinia-affinity-chromatography-protein-purification-system">Profinia&trade; Affinity Chromatography Protein Purification System</a></li> <li><a href="/en-us/category/transfection">Transfection</a></li> <li><a href="/en-us/category/spectrophotometry">Spectrophotometry</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Profinity IMAC Resins <a href="/webroot/web/pdf/lsr/literature/10001677B.PDF">Instruction Manual</a></li> <li>Profinity IMAC Resin Product <a href="/webroot/web/pdf/lsr/literature/Bulletin_3193.pdf">Information Sheet</a></li> <li>Profinity IMAC Ni-Charged Resin <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/Bulletin_5283.pdf" target="_blank">Flier</a></li> <li>Profinity IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/Bulletin_5456.pdf" target="_blank">Tech Note </a></li> <li>Bio-Scale Mini Profinity IMAC Cartridges, 1 and 5 ml <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/10005455.pdf" target="_blank">Instruction Manual</a></li> <li>Bio-Scale Mini Profinity IMAC Cartridge <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/Bulletin_5444A.pdf" target="_blank">Product Information Sheet</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/introduction-transfection">Introduction to Transfection</a></li> <li><a href="/en-us/applications-technologies/histidine-tagged-recombinant-protein-purification-column-refolding?ID=MA7DZ94VY">Histidine-Tagged Recombinant Protein Purification and On-Column Refolding</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated profinity-imac-resin-ni-charged-10ml-1560131.html /webroot/web/html/lsr/products/SKUs/autopopulated/profinity-imac-resin-ni-charged-10ml-1560131.html Ligand 1775 Iminodiacetic acid (IDA) 1 0 Form 1659 50% slurry in 20% ethanol, Ni-charged or uncharged 1 0 Particle size 2763 45–90 μm (mean 60 μm) 1 0 Capacity 1404 12–30 µmol Cu<sup>2+</sup>/ml resin, 6x histidine-tagged proteins ≥15 mg/ml resin (dynamic binding capacity varies from protein to protein) 1 0 Maximum operating pressure 1817 109 psi (7.5 bar) 1 0 Recommended flow rate 2058 ≤600 cm/hr at 25°C 1 0 Operating temperature range 2553 4–40°C 1 0 Autoclavability (121°C, 30 min) 1355 Yes (in 0.1 M sodium acetate) 1 0 Storage 2198 4°C or ambient 1 0 Shelf life 2149 ≥1 year at ambient temperature in 20% ethanol 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560131&CartQuote=QT&quantity= false true false $110.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560131&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560131&quantity= 1560131
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Profinity™ IMAC Resin, Ni-charged, 25 ml

1560133
Pkg of 1, 25 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography

1 1560133 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0133_0921120658478.xml 1560133 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jao0teoujao0teov" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jao0teoujao0teov"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="13" Second="20" ZoneOffsetMillis="0" Time="1512155600373">Fri Dec 01 19:13:20 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0133_0921120658478.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="13" Second="20" ZoneOffsetMillis="0" Time="1512155600373">Fri Dec 01 19:13:20 UTC 2017</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jao0ten4 2017-11-14 07:08:02 true true internet/product_SKU LSR N en 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-01 00:17:45 1560133-profinity-imac-resin-ni-charged-25-ml 1560133 Profinity IMAC Ni-Charged Resin Profinity™ IMAC Resin, Ni-charged, 25 ml Pkg of 1, 25 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography 25 ml, ready-to-use support for immobilized metal affinity chromatography Pkg of 1, 25 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography /webroot/web/images/lsr/products/chromatography/sku_thumb/global/156-0133_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/156-0133_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/156-0133_feature.jpg preview RepEx 156-0133 25 ml, ready-to-use support for immobilized metal affinity chromatography 1560133, profinity, imac, nickel-charged, ni-charged, ni, nickel, charged, resin, immobilized, metal, affinity, chromatography, ready-to-use, support, media, hisitidine, his, tagged, his-tagged, protein, puritifcation, 25, ml 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 <p>Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights.</p> <ul> <li>Chelating ligand is iminodiacetic acid (IDA)</li> <li>Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems</li> <li>Stable across a wide pH range (1&ndash;14)</li> <li>Works under native or denaturing conditions</li> <li>Can be used for single-step purification from crude samples or contaminant removal</li> </ul> <p>Profinity IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.</p> <h2 class="descHeader">Product Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="325" align="left" valign="top"><strong>Resin Form</strong></td> <td width="175" align="center" valign="top"><strong>Package Size </strong></td> <td width="319" align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560131-profinity-imac-resin-ni-charged-10-ml?ID=1560131">1560131</a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">25 ml</td> <td align="center" valign="top">1560133</td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">100 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560135-profinity-imac-resin-ni-charged-100-ml?ID=1560135">1560135 </a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560137-profinity-imac-resin-ni-charged-500-ml?ID=1560137">1560137 </a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560121-profinity-imac-resin-uncharged-10-ml?ID=1560121">1560121 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">50 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560123-profinity-imac-resin-uncharged-50-ml?ID=1560123">1560123</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560125-profinity-imac-resin-uncharged-500-ml?ID=1560125">1560125 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">1 L</td> <td align="center" valign="top"><a href=" /en-us/sku/1560127-profinity-imac-uncharged-resin">1560127</a></td> </tr> </tbody> </table> <p>Prepacked <a href="/en-us/product/bio-scale-mini-profinity-imac-cartridges">Bio-Scale&trade; Mini Profinity&trade; IMAC Cartridges</a> with uncharged or Ni-charged IDA, and <a href="/en-us/product/bio-scale-mini-nuvia-imac-cartridges">Bio-Scale&trade; Mini Nuvia&trade; IMAC Cartridges</a> with Ni-charged NTA are available.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Native IMAC Buffer Kit (<a href="/en-us/sku/6200239-native-imac-buffer-kit">6200239</a>) includes IMAC lysis, wash, and elution buffers</li> <li>Native IMAC Purification Kits, 1 ml (<a href="/en-us/sku/6200241-native-imac-purification-kit-1-ml">6200241</a>) includes Native IMAC buffer Kit and 2x1 ml Bio-Scale Mini IMAC Cartridges, and 5 ml (<a href="/en-us/sku/6200242-native-imac-purification-kit-5-ml">6200242</a>) includes Native IMAC buffer Kit and 1x5 ml Bio-Scale Mini IMAC Cartridge </li> <li><a href="/en-us/product/nuvia-imac-resin">Nuvia IMAC Resin</a>, Ni-charged nitrilotriacetic acid (NTA)</li> <li>Empty Bio-Scale Mini Cartridges, 1 ml (<a href="/en-us/sku/7324660-1-ml-empty-bio-scale-mini-cartridges">7324660</a>) and 5 ml (<a href="/en-us/sku/7324661-5-ml-empty-bio-scale-mini-cartridges">7324661</a>)</li> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>&reg;</sup> </a><a href="/en-us/product/poly-prep-chromatography-columns">Chromatography</a><a href="/en-us/product/poly-prep-chromatography-columns"> Columns</a>, hold up to 2 ml support</li> <li>His Antibody (<a href="/en-us/sku/6200203-his-antibody">6200203</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/ngc-medium-pressure-liquid-chromatography-systems?ID=MFCV7415">NGC&trade; Medium-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic&trade; Low-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/profinia-affinity-chromatography-protein-purification-system">Profinia&trade; Affinity Chromatography Protein Purification System</a></li> <li><a href="/en-us/category/transfection">Transfection</a></li> <li><a href="/en-us/category/spectrophotometry">Spectrophotometry</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Profinity IMAC Resins <a href="/webroot/web/pdf/lsr/literature/10001677B.PDF">Instruction Manual</a></li> <li>Profinity IMAC Resin Product <a href="/webroot/web/pdf/lsr/literature/Bulletin_3193.pdf">Information Sheet</a></li> <li>Profinity IMAC Ni-Charged Resin <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/Bulletin_5283.pdf" target="_blank">Flier</a></li> <li>Profinity IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/Bulletin_5456.pdf" target="_blank">Tech Note </a></li> <li>Bio-Scale Mini Profinity IMAC Cartridges, 1 and 5 ml <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/10005455.pdf" target="_blank">Instruction Manual</a></li> <li>Bio-Scale Mini Profinity IMAC Cartridge <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/Bulletin_5444A.pdf" target="_blank">Product Information Sheet</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/introduction-transfection">Introduction to Transfection</a></li> <li><a href="/en-us/applications-technologies/histidine-tagged-recombinant-protein-purification-column-refolding?ID=MA7DZ94VY">Histidine-Tagged Recombinant Protein Purification and On-Column Refolding</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated profinity-imac-resin-ni-charged-25ml-1560133.html /webroot/web/html/lsr/products/SKUs/autopopulated/profinity-imac-resin-ni-charged-25ml-1560133.html Ligand 1775 Iminodiacetic acid (IDA) 1 0 Form 1659 50% slurry in 20% ethanol, Ni-charged 1 0 Particle size 2763 45–90 μm (mean 60 μm) 1 0 Capacity 1404 12–30 µmol Cu<sup>2+</sup>/ml resin, 6x histidine-tagged proteins ≥15 mg/ml resin (dynamic binding capacity varies from protein to protein) 1 0 Maximum operating pressure 1817 109 psi (7.5 bar) 1 0 Recommended flow rate 2058 ≤600 cm/hr at 25°C 1 0 Operating temperature range 2553 4–40°C 1 0 Autoclavability (121°C, 30 min) 1355 Yes (in 0.1 M sodium acetate) 1 0 Storage 2198 4°C or ambient 1 0 Shelf life 2149 ≥1 year at ambient temperature in 20% ethanol 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560133&CartQuote=QT&quantity= false true false $268.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560133&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560133&quantity= 1560133
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Profinity™ IMAC Resin, Ni-charged, 100 ml

1560135
Pkg of 1, 100 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography

2 1560135 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0135_0921120658449.xml 1560135 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jao0teoujao0teov" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jao0teoujao0teov"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="13" Second="20" ZoneOffsetMillis="0" Time="1512155600373">Fri Dec 01 19:13:20 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0135_0921120658449.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="13" Second="20" ZoneOffsetMillis="0" Time="1512155600373">Fri Dec 01 19:13:20 UTC 2017</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jao0teoq 2017-11-14 07:08:02 true true internet/product_SKU LSR N en 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-01 00:17:45 1560135-profinity-imac-resin-ni-charged-100-ml 1560135 Profinity IMAC Ni-Charged Resin Profinity™ IMAC Resin, Ni-charged, 100 ml Pkg of 1, 100 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography 100 ml, ready-to-use support for immobilized metal affinity chromatography Pkg of 1, 100 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography /webroot/web/images/ps/products/affinity_media/sku_thumb/global/156-0135_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/156-0135_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/156-0135_feature.jpg preview RepEx 156-0135 100 ml, ready-to-use support for immobilized metal affinity chromatography 1560135, profinity, imac, nickel-charged, ni-charged, ni, nickel, charged, resin, immobilized, metal, affinity, chromatography, ready-to-use, support, media, hisitidine, his, tagged, his-tagged, protein, puritifcation, 100, ml 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 <p>Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights.</p> <ul> <li>Chelating ligand is iminodiacetic acid (IDA)</li> <li>Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems</li> <li>Stable across a wide pH range (1&ndash;14)</li> <li>Works under native or denaturing conditions</li> <li>Can be used for single-step purification from crude samples or contaminant removal</li> </ul> <p>Profinity IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.</p> <h2 class="descHeader">Product Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="325" align="left" valign="top"><strong>Resin Form</strong></td> <td width="175" align="center" valign="top"><strong>Package Size </strong></td> <td width="319" align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560131-profinity-imac-resin-ni-charged-10-ml?ID=1560131">1560131</a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">25 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560133-profinity-imac-resin-ni-charged-25-ml?ID=1560133">1560133</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">100 ml</td> <td align="center" valign="top">1560135</td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560137-profinity-imac-resin-ni-charged-500-ml?ID=1560137">1560137 </a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560121-profinity-imac-resin-uncharged-10-ml?ID=1560121">1560121 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">50 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560123-profinity-imac-resin-uncharged-50-ml?ID=1560123">1560123</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560125-profinity-imac-resin-uncharged-500-ml?ID=1560125">1560125 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">1 L</td> <td align="center" valign="top"><a href=" /en-us/sku/1560127-profinity-imac-uncharged-resin">1560127</a></td> </tr> </tbody> </table> <p>Prepacked <a href="/en-us/product/bio-scale-mini-profinity-imac-cartridges">Bio-Scale&trade; Mini Profinity&trade; IMAC Cartridges</a> with uncharged or Ni-charged IDA, and <a href="/en-us/product/bio-scale-mini-nuvia-imac-cartridges">Bio-Scale&trade; Mini Nuvia&trade; IMAC Cartridges</a> with Ni-charged NTA are available.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Native IMAC Buffer Kit (<a href="/en-us/sku/6200239-native-imac-buffer-kit">6200239</a>) includes IMAC lysis, wash, and elution buffers</li> <li>Native IMAC Purification Kits, 1 ml (<a href="/en-us/sku/6200241-native-imac-purification-kit-1-ml">6200241</a>) includes Native IMAC buffer Kit and 2x1 ml Bio-Scale Mini IMAC Cartridges, and 5 ml (<a href="/en-us/sku/6200242-native-imac-purification-kit-5-ml">6200242</a>) includes Native IMAC buffer Kit and 1x5 ml Bio-Scale Mini IMAC Cartridge </li> <li><a href="/en-us/product/nuvia-imac-resin">Nuvia IMAC Resin</a>, Ni-charged nitrilotriacetic acid (NTA)</li> <li>Empty Bio-Scale Mini Cartridges, 1 ml (<a href="/en-us/sku/7324660-1-ml-empty-bio-scale-mini-cartridges">7324660</a>) and 5 ml (<a href="/en-us/sku/7324661-5-ml-empty-bio-scale-mini-cartridges">7324661</a>)</li> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>&reg;</sup> </a><a href="/en-us/product/poly-prep-chromatography-columns">Chromatography</a><a href="/en-us/product/poly-prep-chromatography-columns"> Columns</a>, hold up to 2 ml support</li> <li>His Antibody (<a href="/en-us/sku/6200203-his-antibody">6200203</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/ngc-medium-pressure-liquid-chromatography-systems?ID=MFCV7415">NGC&trade; Medium-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic&trade; Low-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/profinia-affinity-chromatography-protein-purification-system">Profinia&trade; Affinity Chromatography Protein Purification System</a></li> <li><a href="/en-us/category/transfection">Transfection</a></li> <li><a href="/en-us/category/spectrophotometry">Spectrophotometry</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Profinity IMAC Resins <a href="/webroot/web/pdf/lsr/literature/10001677B.PDF">Instruction Manual</a></li> <li>Profinity IMAC Resin Product <a href="/webroot/web/pdf/lsr/literature/Bulletin_3193.pdf">Information Sheet</a></li> <li>Profinity IMAC Ni-Charged Resin <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/Bulletin_5283.pdf" target="_blank">Flier</a></li> <li>Profinity IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/Bulletin_5456.pdf" target="_blank">Tech Note </a></li> <li>Bio-Scale Mini Profinity IMAC Cartridges, 1 and 5 ml <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/10005455.pdf" target="_blank">Instruction Manual</a></li> <li>Bio-Scale Mini Profinity IMAC Cartridge <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/Bulletin_5444A.pdf" target="_blank">Product Information Sheet</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/introduction-transfection">Introduction to Transfection</a></li> <li><a href="/en-us/applications-technologies/histidine-tagged-recombinant-protein-purification-column-refolding?ID=MA7DZ94VY">Histidine-Tagged Recombinant Protein Purification and On-Column Refolding</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated profinity-imac-resin-ni-charged-100ml-1560135.html /webroot/web/html/lsr/products/SKUs/autopopulated/profinity-imac-resin-ni-charged-100ml-1560135.html Ligand 1775 Iminodiacetic acid (IDA) 1 0 Form 1659 50% slurry in 20% ethanol, Ni-charged 1 0 Particle size 2763 45–90 μm (mean 60 μm) 1 0 Capacity 1404 12–30 µmol Cu<sup>2+</sup>/ml resin, 6x histidine-tagged proteins ≥15 mg/ml resin (dynamic binding capacity varies from protein to protein) 1 0 Maximum operating pressure 1817 109 psi (7.5 bar) 1 0 Recommended flow rate 2058 ≤600 cm/hr at 25°C 1 0 Operating temperature range 2553 4–40°C 1 0 Autoclavability (121°C, 30 min) 1355 Yes (in 0.1 M sodium acetate) 1 0 Storage 2198 4°C or ambient 1 0 Shelf life 2149 ≥1 year at ambient temperature in 20% ethanol 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560135&CartQuote=QT&quantity= false true false $982.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560135&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560135&quantity= 1560135
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Profinity™ IMAC Resin, Ni-charged, 500 ml

1560137
Pkg of 1, 500 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography

3 1560137 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0137_0921120658390.xml 1560137 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jao0teoujao0teov" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jao0teoujao0teov"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="13" Second="20" ZoneOffsetMillis="0" Time="1512155600373">Fri Dec 01 19:13:20 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0137_0921120658390.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="13" Second="20" ZoneOffsetMillis="0" Time="1512155600373">Fri Dec 01 19:13:20 UTC 2017</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jao0temt 2017-11-14 07:08:02 true true internet/product_SKU LSR N en 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-01 00:17:45 1560137-profinity-imac-resin-ni-charged-500-ml 1560137 Profinity IMAC Ni-Charged Resin Profinity™ IMAC Resin, Ni-charged, 500 ml Pkg of 1, 500 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography 500 ml, ready-to-use support for immobilized metal affinity chromatography Pkg of 1, 500 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography /webroot/web/images/ps/products/affinity_media/sku_thumb/global/156-0137_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/156-0137_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/156-0137_feature.jpg preview RepEx 156-0137 500 ml, ready-to-use support for immobilized metal affinity chromatography 1560137, profinity, imac, nickel-charged, ni-charged, ni, nickel, charged, resin, immobilized, metal, affinity, chromatography, ready-to-use, support, media, hisitidine, his, tagged, his-tagged, protein, puritifcation, 500, ml 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 <p>Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights.</p> <ul> <li>Chelating ligand is iminodiacetic acid (IDA)</li> <li>Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems</li> <li>Stable across a wide pH range (1&ndash;14)</li> <li>Works under native or denaturing conditions</li> <li>Can be used for single-step purification from crude samples or contaminant removal</li> </ul> <p>Profinity IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.</p> <h2 class="descHeader">Product Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="325" align="left" valign="top"><strong>Resin Form</strong></td> <td width="175" align="center" valign="top"><strong>Package Size </strong></td> <td width="319" align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560131-profinity-imac-resin-ni-charged-10-ml?ID=1560131">1560131</a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">25 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560133-profinity-imac-resin-ni-charged-25-ml?ID=1560133">1560133</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">100 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560135-profinity-imac-resin-ni-charged-100-ml?ID=1560135">1560135 </a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top">1560137</td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560121-profinity-imac-resin-uncharged-10-ml?ID=1560121">1560121 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">50 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560123-profinity-imac-resin-uncharged-50-ml?ID=1560123">1560123</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560125-profinity-imac-resin-uncharged-500-ml?ID=1560125">1560125 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">1 L</td> <td align="center" valign="top"><a href=" /en-us/sku/1560127-profinity-imac-uncharged-resin">1560127</a></td> </tr> </tbody> </table> <p>Prepacked <a href="/en-us/product/bio-scale-mini-profinity-imac-cartridges">Bio-Scale&trade; Mini Profinity&trade; IMAC Cartridges</a> with uncharged or Ni-charged IDA, and <a href="/en-us/product/bio-scale-mini-nuvia-imac-cartridges">Bio-Scale&trade; Mini Nuvia&trade; IMAC Cartridges</a> with Ni-charged NTA are available.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Native IMAC Buffer Kit (<a href="/en-us/sku/6200239-native-imac-buffer-kit">6200239</a>) includes IMAC lysis, wash, and elution buffers</li> <li>Native IMAC Purification Kits, 1 ml (<a href="/en-us/sku/6200241-native-imac-purification-kit-1-ml">6200241</a>) includes Native IMAC buffer Kit and 2x1 ml Bio-Scale Mini IMAC Cartridges, and 5 ml (<a href="/en-us/sku/6200242-native-imac-purification-kit-5-ml">6200242</a>) includes Native IMAC buffer Kit and 1x5 ml Bio-Scale Mini IMAC Cartridge </li> <li><a href="/en-us/product/nuvia-imac-resin">Nuvia IMAC Resin</a>, Ni-charged nitrilotriacetic acid (NTA)</li> <li>Empty Bio-Scale Mini Cartridges, 1 ml (<a href="/en-us/sku/7324660-1-ml-empty-bio-scale-mini-cartridges">7324660</a>) and 5 ml (<a href="/en-us/sku/7324661-5-ml-empty-bio-scale-mini-cartridges">7324661</a>)</li> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>&reg;</sup> </a><a href="/en-us/product/poly-prep-chromatography-columns">Chromatography</a><a href="/en-us/product/poly-prep-chromatography-columns"> Columns</a>, hold up to 2 ml support</li> <li>His Antibody (<a href="/en-us/sku/6200203-his-antibody">6200203</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/ngc-medium-pressure-liquid-chromatography-systems?ID=MFCV7415">NGC&trade; Medium-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic&trade; Low-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/profinia-affinity-chromatography-protein-purification-system">Profinia&trade; Affinity Chromatography Protein Purification System</a></li> <li><a href="/en-us/category/transfection">Transfection</a></li> <li><a href="/en-us/category/spectrophotometry">Spectrophotometry</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Profinity IMAC Resins <a href="/webroot/web/pdf/lsr/literature/10001677B.PDF">Instruction Manual</a></li> <li>Profinity IMAC Resin Product <a href="/webroot/web/pdf/lsr/literature/Bulletin_3193.pdf">Information Sheet</a></li> <li>Profinity IMAC Ni-Charged Resin <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/Bulletin_5283.pdf" target="_blank">Flier</a></li> <li>Profinity IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/Bulletin_5456.pdf" target="_blank">Tech Note </a></li> <li>Bio-Scale Mini Profinity IMAC Cartridges, 1 and 5 ml <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/10005455.pdf" target="_blank">Instruction Manual</a></li> <li>Bio-Scale Mini Profinity IMAC Cartridge <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/Bulletin_5444A.pdf" target="_blank">Product Information Sheet</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/introduction-transfection">Introduction to Transfection</a></li> <li><a href="/en-us/applications-technologies/histidine-tagged-recombinant-protein-purification-column-refolding?ID=MA7DZ94VY">Histidine-Tagged Recombinant Protein Purification and On-Column Refolding</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated profinity-imac-resin-ni-charged-500ml-1560137.html /webroot/web/html/lsr/products/SKUs/autopopulated/profinity-imac-resin-ni-charged-500ml-1560137.html Ligand 1775 Iminodiacetic acid (IDA) 1 0 Form 1659 50% slurry in 20% ethanol, Ni-charged 1 0 Particle size 2763 45–90 μm (mean 60 μm) 1 0 Capacity 1404 12–30 µmol Cu<sup>2+</sup>/ml resin, 6x histidine-tagged proteins ≥15 mg/ml resin (dynamic binding capacity varies from protein to protein) 1 0 Maximum operating pressure 1817 109 psi (7.5 bar) 1 0 Recommended flow rate 2058 ≤600 cm/hr at 25°C 1 0 Operating temperature range 2553 4–40°C 1 0 Autoclavability (121°C, 30 min) 1355 Yes (in 0.1 M sodium acetate) 1 0 Storage 2198 4°C or ambient 1 0 Shelf life 2149 ≥1 year at ambient temperature in 20% ethanol 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560137&CartQuote=QT&quantity= false true false $4,426.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560137&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560137&quantity= 1560137
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Profinity™ IMAC Resin, uncharged, 10 ml

1560121
Pkg of 1, 10 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography

4 1560121 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0121_0921120658495.xml 1560121 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jao0teoujao0teov" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jao0teoujao0teov"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="13" Second="20" ZoneOffsetMillis="0" Time="1512155600373">Fri Dec 01 19:13:20 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0121_0921120658495.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="13" Second="20" ZoneOffsetMillis="0" Time="1512155600373">Fri Dec 01 19:13:20 UTC 2017</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jao0teo5 2017-11-14 07:08:02 true true internet/product_SKU LSR N en 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-01 00:17:45 1560121-profinity-imac-resin-uncharged-10-ml 1560121 Profinity IMAC Uncharged Resin Profinity™ IMAC Resin, uncharged, 10 ml Pkg of 1, 10 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography 10 ml, ready-to-use support for immobilized metal affinity chromatography Pkg of 1, 10 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography /webroot/web/images/lsr/products/chromatography/sku_thumb/global/156-0121_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/156-0121_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/156-0121_feature.jpg preview RepEx 156-0121 10 ml, ready-to-use support for immobilized metal affinity chromatography 1560121, profinity, imac, uncharged, resin, immobilized, metal, affinity, chromatography, purification, ready-to-use, support, media, ml 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 <p>Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights.</p> <ul> <li>Chelating ligand is iminodiacetic acid (IDA)</li> <li>Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems</li> <li>Stable across a wide pH range (1&ndash;14)</li> <li>Works under native or denaturing conditions</li> <li>Can be used for single-step purification from crude samples or contaminant removal</li> </ul> <p>Profinity IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.</p> <h2 class="descHeader">Product Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="325" align="left" valign="top"><strong>Resin Form</strong></td> <td width="175" align="center" valign="top"><strong>Package Size </strong></td> <td width="319" align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560131-profinity-imac-resin-ni-charged-10-ml?ID=1560131">1560131</a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">25 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560133-profinity-imac-resin-ni-charged-25-ml?ID=1560133">1560133</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">100 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560135-profinity-imac-resin-ni-charged-100-ml?ID=1560135">1560135 </a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560137-profinity-imac-resin-ni-charged-500-ml?ID=1560137">1560137 </a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top">1560121</td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">50 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560123-profinity-imac-resin-uncharged-50-ml?ID=1560123">1560123</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560125-profinity-imac-resin-uncharged-500-ml?ID=1560125">1560125 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">1 L</td> <td align="center" valign="top"><a href=" /en-us/sku/1560127-profinity-imac-uncharged-resin">1560127</a></td> </tr> </tbody> </table> <p>Prepacked <a href="/en-us/product/bio-scale-mini-profinity-imac-cartridges">Bio-Scale&trade; Mini Profinity&trade; IMAC Cartridges</a> with uncharged or Ni-charged IDA, and <a href="/en-us/product/bio-scale-mini-nuvia-imac-cartridges">Bio-Scale&trade; Mini Nuvia&trade; IMAC Cartridges</a> with Ni-charged NTA are available.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Native IMAC Buffer Kit (<a href="/en-us/sku/6200239-native-imac-buffer-kit">6200239</a>) includes IMAC lysis, wash, and elution buffers</li> <li>Native IMAC Purification Kits, 1 ml (<a href="/en-us/sku/6200241-native-imac-purification-kit-1-ml">6200241</a>) includes Native IMAC buffer Kit and 2x1 ml Bio-Scale Mini IMAC Cartridges, and 5 ml (<a href="/en-us/sku/6200242-native-imac-purification-kit-5-ml">6200242</a>) includes Native IMAC buffer Kit and 1x5 ml Bio-Scale Mini IMAC Cartridge </li> <li><a href="/en-us/product/nuvia-imac-resin">Nuvia IMAC Resin</a>, Ni-charged nitrilotriacetic acid (NTA)</li> <li>Empty Bio-Scale Mini Cartridges, 1 ml (<a href="/en-us/sku/7324660-1-ml-empty-bio-scale-mini-cartridges">7324660</a>) and 5 ml (<a href="/en-us/sku/7324661-5-ml-empty-bio-scale-mini-cartridges">7324661</a>)</li> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>&reg;</sup> </a><a href="/en-us/product/poly-prep-chromatography-columns">Chromatography</a><a href="/en-us/product/poly-prep-chromatography-columns"> Columns</a>, hold up to 2 ml support</li> <li>His Antibody (<a href="/en-us/sku/6200203-his-antibody">6200203</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/ngc-medium-pressure-liquid-chromatography-systems?ID=MFCV7415">NGC&trade; Medium-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic&trade; Low-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/profinia-affinity-chromatography-protein-purification-system">Profinia&trade; Affinity Chromatography Protein Purification System</a></li> <li><a href="/en-us/category/transfection">Transfection</a></li> <li><a href="/en-us/category/spectrophotometry">Spectrophotometry</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Profinity IMAC Resins <a href="/webroot/web/pdf/lsr/literature/10001677B.PDF">Instruction Manual</a></li> <li>Profinity IMAC Resin Product <a href="/webroot/web/pdf/lsr/literature/Bulletin_3193.pdf">Information Sheet</a></li> <li>Profinity IMAC Ni-Charged Resin <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/Bulletin_5283.pdf" target="_blank">Flier</a></li> <li>Profinity IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/Bulletin_5456.pdf" target="_blank">Tech Note </a></li> <li>Bio-Scale Mini Profinity IMAC Cartridges, 1 and 5 ml <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/10005455.pdf" target="_blank">Instruction Manual</a></li> <li>Bio-Scale Mini Profinity IMAC Cartridge <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/Bulletin_5444A.pdf" target="_blank">Product Information Sheet</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/introduction-transfection">Introduction to Transfection</a></li> <li><a href="/en-us/applications-technologies/histidine-tagged-recombinant-protein-purification-column-refolding?ID=MA7DZ94VY">Histidine-Tagged Recombinant Protein Purification and On-Column Refolding</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated profinity-imac-resin-uncharged-10ml-1560121.html /webroot/web/html/lsr/products/SKUs/autopopulated/profinity-imac-resin-uncharged-10ml-1560121.html Ligand 1775 Iminodiacetic acid (IDA) 1 0 Form 1659 50% slurry in 20% ethanol, uncharged 1 0 Particle size 2763 45–90 μm (mean 60 μm) 1 0 Capacity 1404 12–30 µmol Cu<sup>2+</sup>/ml resin, 6x histidine-tagged proteins ≥15 mg/ml resin (dynamic binding capacity varies from protein to protein) 1 0 Maximum operating pressure 1817 109 psi (7.5 bar) 1 0 Recommended flow rate 2058 ≤600 cm/hr at 25°C 1 0 Operating temperature range 2553 4–40°C 1 0 Autoclavability (121°C, 30 min) 1355 Yes (in 0.1 M sodium acetate) 1 0 Storage 2198 4°C or ambient 1 0 Shelf life 2149 ≥1 year at ambient temperature in 20% ethanol 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560121&CartQuote=QT&quantity= false true false $87.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560121&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560121&quantity= 1560121
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Profinity™ IMAC Resin, uncharged, 50 ml

1560123
Pkg of 1, 50 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography

5 1560123 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0123_0921120658518.xml 1560123 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jao0teoujao0teov" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jao0teoujao0teov"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="13" Second="20" ZoneOffsetMillis="0" Time="1512155600373">Fri Dec 01 19:13:20 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0123_0921120658518.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="13" Second="20" ZoneOffsetMillis="0" Time="1512155600373">Fri Dec 01 19:13:20 UTC 2017</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jao0temv 2017-11-14 07:08:02 true true internet/product_SKU LSR N en 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-01 00:17:45 1560123-profinity-imac-resin-uncharged-50-ml 1560123 Profinity IMAC Uncharged Resin Profinity™ IMAC Resin, uncharged, 50 ml Pkg of 1, 50 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography 50 ml, ready-to-use support for immobilized metal affinity chromatography Pkg of 1, 50 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography /webroot/web/images/lsr/products/chromatography/sku_thumb/global/156-0123_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/156-0123_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/156-0123_feature.jpg preview RepEx 156-0123 50 ml, ready-to-use support for immobilized metal affinity chromatography 1560123, profinity, imac, uncharged, resin, immobilized, metal, affinity, chromatography, purification, ready-to-use, support, media, 50, ml 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 <p>Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights.</p> <ul> <li>Chelating ligand is iminodiacetic acid (IDA)</li> <li>Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems</li> <li>Stable across a wide pH range (1&ndash;14)</li> <li>Works under native or denaturing conditions</li> <li>Can be used for single-step purification from crude samples or contaminant removal</li> </ul> <p>Profinity IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.</p> <h2 class="descHeader">Product Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="325" align="left" valign="top"><strong>Resin Form</strong></td> <td width="175" align="center" valign="top"><strong>Package Size </strong></td> <td width="319" align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560131-profinity-imac-resin-ni-charged-10-ml?ID=1560131">1560131</a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">25 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560133-profinity-imac-resin-ni-charged-25-ml?ID=1560133">1560133</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">100 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560135-profinity-imac-resin-ni-charged-100-ml?ID=1560135">1560135 </a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560137-profinity-imac-resin-ni-charged-500-ml?ID=1560137">1560137 </a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560121-profinity-imac-resin-uncharged-10-ml?ID=1560121">1560121 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">50 ml</td> <td align="center" valign="top">1560123</td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560125-profinity-imac-resin-uncharged-500-ml?ID=1560125">1560125 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">1 L</td> <td align="center" valign="top"><a href=" /en-us/sku/1560127-profinity-imac-uncharged-resin">1560127</a></td> </tr> </tbody> </table> <p>Prepacked <a href="/en-us/product/bio-scale-mini-profinity-imac-cartridges">Bio-Scale&trade; Mini Profinity&trade; IMAC Cartridges</a> with uncharged or Ni-charged IDA, and <a href="/en-us/product/bio-scale-mini-nuvia-imac-cartridges">Bio-Scale&trade; Mini Nuvia&trade; IMAC Cartridges</a> with Ni-charged NTA are available.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Native IMAC Buffer Kit (<a href="/en-us/sku/6200239-native-imac-buffer-kit">6200239</a>) includes IMAC lysis, wash, and elution buffers</li> <li>Native IMAC Purification Kits, 1 ml (<a href="/en-us/sku/6200241-native-imac-purification-kit-1-ml">6200241</a>) includes Native IMAC buffer Kit and 2x1 ml Bio-Scale Mini IMAC Cartridges, and 5 ml (<a href="/en-us/sku/6200242-native-imac-purification-kit-5-ml">6200242</a>) includes Native IMAC buffer Kit and 1x5 ml Bio-Scale Mini IMAC Cartridge </li> <li><a href="/en-us/product/nuvia-imac-resin">Nuvia IMAC Resin</a>, Ni-charged nitrilotriacetic acid (NTA)</li> <li>Empty Bio-Scale Mini Cartridges, 1 ml (<a href="/en-us/sku/7324660-1-ml-empty-bio-scale-mini-cartridges">7324660</a>) and 5 ml (<a href="/en-us/sku/7324661-5-ml-empty-bio-scale-mini-cartridges">7324661</a>)</li> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>&reg;</sup> </a><a href="/en-us/product/poly-prep-chromatography-columns">Chromatography</a><a href="/en-us/product/poly-prep-chromatography-columns"> Columns</a>, hold up to 2 ml support</li> <li>His Antibody (<a href="/en-us/sku/6200203-his-antibody">6200203</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/ngc-medium-pressure-liquid-chromatography-systems?ID=MFCV7415">NGC&trade; Medium-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic&trade; Low-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/profinia-affinity-chromatography-protein-purification-system">Profinia&trade; Affinity Chromatography Protein Purification System</a></li> <li><a href="/en-us/category/transfection">Transfection</a></li> <li><a href="/en-us/category/spectrophotometry">Spectrophotometry</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Profinity IMAC Resins <a href="/webroot/web/pdf/lsr/literature/10001677B.PDF">Instruction Manual</a></li> <li>Profinity IMAC Resin Product <a href="/webroot/web/pdf/lsr/literature/Bulletin_3193.pdf">Information Sheet</a></li> <li>Profinity IMAC Ni-Charged Resin <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/Bulletin_5283.pdf" target="_blank">Flier</a></li> <li>Profinity IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/Bulletin_5456.pdf" target="_blank">Tech Note </a></li> <li>Bio-Scale Mini Profinity IMAC Cartridges, 1 and 5 ml <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/10005455.pdf" target="_blank">Instruction Manual</a></li> <li>Bio-Scale Mini Profinity IMAC Cartridge <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/Bulletin_5444A.pdf" target="_blank">Product Information Sheet</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/introduction-transfection">Introduction to Transfection</a></li> <li><a href="/en-us/applications-technologies/histidine-tagged-recombinant-protein-purification-column-refolding?ID=MA7DZ94VY">Histidine-Tagged Recombinant Protein Purification and On-Column Refolding</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated profinity-imac-resin-uncharged-50ml-1560123.html /webroot/web/html/lsr/products/SKUs/autopopulated/profinity-imac-resin-uncharged-50ml-1560123.html Ligand 1775 1 0 Form 1659 50% slurry in 20% ethanol, uncharged 1 0 Particle size 2763 45–90 μm (mean 60 μm) 1 0 Capacity 1404 12–30 µmol Cu<sup>2+</sup>/ml resin, 6x histidine-tagged proteins ≥15 mg/ml resin (dynamic binding capacity varies from protein to protein) 1 0 Maximum operating pressure 1817 109 psi (7.5 bar) 1 0 Recommended flow rate 2058 ≤600 cm/hr at 25°C 1 0 Operating temperature range 2553 4–40°C 1 0 Autoclavability (121°C, 30 min) 1355 Yes (in 0.1 M sodium acetate) 1 0 Storage 2198 4°C or ambient 1 0 Shelf life 2149 ≥1 year at ambient temperature in 20% ethanol 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560123&CartQuote=QT&quantity= false true false $378.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560123&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560123&quantity= 1560123
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Profinity™ IMAC Resin, uncharged, 500 ml

1560125
Pkg of 1, 500 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography

This product is not available for sale online. Please contact customer service for more information.
Profinity™ IMAC Resin, uncharged, 1 L

1560127
Pkg of 1, 1 L, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography

7 1560127 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0127_0921120658537.xml 1560127 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jao0teoujao0teov" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jao0teoujao0teov"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="13" Second="20" ZoneOffsetMillis="0" Time="1512155600373">Fri Dec 01 19:13:20 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0127_0921120658537.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="13" Second="20" ZoneOffsetMillis="0" Time="1512155600373">Fri Dec 01 19:13:20 UTC 2017</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jao0teni 2017-11-14 07:08:02 true true internet/product_SKU LSR N en 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-01 00:17:45 1560127-profinity-imac-resin-uncharged-1-l 1560127 Profinity IMAC Uncharged Resin Profinity™ IMAC Resin, uncharged, 1 L Pkg of 1, 1 L, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography 1 L, ready-to-use support for immobilized metal affinity chromatography Pkg of 1, 1 L, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography /webroot/web/images/lsr/products/chromatography/sku_thumb/global/156-0127_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/156-0127_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/156-0127_feature.jpg preview RepEx 156-0127 1 L, ready-to-use support for immobilized metal affinity chromatography 1560127, profinity, imac, uncharged, resin, immobilized, metal, affinity, chromatography, purification, ready-to-use, support, media, 1L 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 <p>Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights.</p> <ul> <li>Chelating ligand is iminodiacetic acid (IDA)</li> <li>Use with batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems</li> <li>Stable across a wide pH range (1&ndash;14)</li> <li>Use under native or denaturing conditions</li> <li>Single-step purification from crude samples, or use for contaminant removal</li> </ul> <p>Profinity IMAC Resin is compatible with denaturants (including 8M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.</p> <h2 class="descHeader">Product Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="325" align="left" valign="top"><strong>Resin Form</strong></td> <td width="175" align="center" valign="top"><strong>Package Size </strong></td> <td width="319" align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560131-profinity-imac-resin-ni-charged-10-ml?ID=1560131">1560131</a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">25 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560133-profinity-imac-resin-ni-charged-25-ml?ID=1560133">1560133</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">100 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560135-profinity-imac-resin-ni-charged-100-ml?ID=1560135">1560135 </a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560137-profinity-imac-resin-ni-charged-500-ml?ID=1560137">1560137 </a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560121-profinity-imac-resin-uncharged-10-ml?ID=1560121">1560121 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">50 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560123-profinity-imac-resin-uncharged-50-ml?ID=1560123">1560123</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560125-profinity-imac-resin-uncharged-500-ml?ID=1560125">1560125 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">1 L</td> <td align="center" valign="top">1560127</td> </tr> </tbody> </table> <p>Prepacked <a href="/en-us/product/bio-scale-mini-profinity-imac-cartridges">Bio-Scale&trade; Mini Profinity&trade; IMAC Cartridges</a> with uncharged or Ni-charged IDA, and <a href="/en-us/product/bio-scale-mini-nuvia-imac-cartridges">Bio-Scale&trade; Mini Nuvia&trade; IMAC Cartridges</a> with Ni-charged NTA are available.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Native IMAC Buffer Kit (<a href="/en-us/sku/6200239-native-imac-buffer-kit">6200239</a>) includes IMAC lysis, wash, and elution buffers</li> <li>Native IMAC Purification Kits, 1 ml (<a href="/en-us/sku/6200241-native-imac-purification-kit-1-ml">6200241</a>) includes Native IMAC buffer Kit and 2x1 ml Bio-Scale Mini IMAC Cartridges, and 5 ml (<a href="/en-us/sku/6200242-native-imac-purification-kit-5-ml">6200242</a>) includes Native IMAC buffer Kit and 1x5 ml Bio-Scale Mini IMAC Cartridge </li> <li><a href="/en-us/product/nuvia-imac-resin">Nuvia IMAC Resin</a>, Ni-charged nitrilotriacetic acid (NTA)</li> <li>Empty Bio-Scale Mini Cartridges, 1 ml (<a href="/en-us/sku/7324660-1-ml-empty-bio-scale-mini-cartridges">7324660</a>) and 5 ml (<a href="/en-us/sku/7324661-5-ml-empty-bio-scale-mini-cartridges">7324661</a>)</li> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>&reg;</sup> </a><a href="/en-us/product/poly-prep-chromatography-columns">Chromatography</a><a href="/en-us/product/poly-prep-chromatography-columns"> Columns</a>, hold up to 2 ml support</li> <li>His Antibody (<a href="/en-us/sku/6200203-his-antibody">6200203</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/ngc-medium-pressure-liquid-chromatography-systems?ID=MFCV7415">NGC&trade; Medium-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic&trade; Low-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/profinia-affinity-chromatography-protein-purification-system">Profinia&trade; Affinity Chromatography Protein Purification System</a></li> <li><a href="/en-us/category/transfection">Transfection</a></li> <li><a href="/en-us/category/spectrophotometry">Spectrophotometry</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Profinity IMAC Resins <a href="/webroot/web/pdf/lsr/literature/10001677B.PDF">Instruction Manual</a></li> <li>Profinity IMAC Resin Product <a href="/webroot/web/pdf/lsr/literature/Bulletin_3193.pdf">Information Sheet</a></li> <li>Profinity IMAC Ni-Charged Resin <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/Bulletin_5283.pdf" target="_blank">Flier</a></li> <li>Profinity IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/Bulletin_5456.pdf" target="_blank">Tech Note </a></li> <li>Bio-Scale Mini Profinity IMAC Cartridges, 1 and 5 ml <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/10005455.pdf" target="_blank">Instruction Manual</a></li> <li>Bio-Scale Mini Profinity IMAC Cartridge <a class="no-target-style" title="Link opens in new window" href="Link to /webroot/web/pdf/lsr/literature/Bulletin_5444A.pdf" target="_blank">Product Information Sheet</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/introduction-transfection">Introduction to Transfection</a></li> <li><a href="/en-us/applications-technologies/histidine-tagged-recombinant-protein-purification-column-refolding?ID=MA7DZ94VY">Histidine-Tagged Recombinant Protein Purification and On-Column Refolding</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated profinity-imac-resin-uncharged-1l-1560127.html /webroot/web/html/lsr/products/SKUs/autopopulated/profinity-imac-resin-uncharged-1l-1560127.html Ligand 1775 Iminodiacetic acid (IDA) 1 0 Form 1659 50% slurry in 20% ethanol, uncharged 1 0 Particle size 2763 45–90 μm (mean 60 μm) 1 0 Capacity 1404 12–30 µmol Cu<sup>2+</sup>/ml resin, 6x histidine-tagged proteins ≥15 mg/ml resin (dynamic binding capacity varies from protein to protein) 1 0 Maximum operating pressure 1817 109 psi (7.5 bar) 1 0 Recommended flow rate 2058 ≤600 cm/hr at 25°C 1 0 Operating temperature range 2553 4–40°C 1 0 Autoclavability (121°C, 30 min) 1355 Yes (in 0.1 M sodium acetate) 1 0 Storage 2198 4°C or ambient 1 0 Shelf life 2149 ≥1 year at ambient temperature in 20% ethanol 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560127&CartQuote=QT&quantity= false true false null false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560127&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560127&quantity= 1560127
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Number Description Options
5456 Profinity™ IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification, Rev B Click to download
10001677 Instruction Manual, Profinity IMAC Resins, Rev B Click to download
3193 Profinity IMAC Resin Product Information Sheet Click to download
5283 Profinity IMAC Ni-Charged Resin Flier, Rev A Click to download
5925 Affinity Purification Buffer Kit Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]

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