IEP/IEF Agaroses

IEP/IEF Agaroses

Overview

Bio-Rad offers a selection of agaroses suitable for IEP, IEF, and other specialty applications. Standard low, high, and zero -mr agarose are ideal for immuno-, counterimmunoelectrophoresis and IEF.
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Description

Description



Bio-Rad offers a selection of agaroses suitable for IEP, IEF, and other specialty applications.

Standard low –mr agarose — With its high strength, clarity, and low –mr value, this agarose is recommended for all standard immunoelectrophoresis applications. The powder may be used alone or blended with other grades of agarose for special properties.

High –mr agarose — The –mr value of ≥0.25 makes this gel suitable for counterimmunoelectrophoresis and to blend with standard low –mr agarose to increase its –mr for special applications.

Zero –mr agarose — This agarose is specifically for isoelectric focusing. It has no detectable electroendosmosis, and is recommended for isoelectric focusing of very high molecular weight proteins or complexes that are subject to varying degrees of molecular sieving in polyacrylamide gels. Agarose IEF and post-run processing can be completed more quickly than polyacrylamide gel IEF.

Specifications

Specifications

Electroendosmosis
–mr (1.0% Gel)
 
Gel Strength
(g/cm2 of a 1.0% Gel)
Standard low –mr ≤0.15
Standard low –mr ≥1,000
High –mr ≥0.25
High –mr ≥450
Zero –mr ≤0.02
Zero –mr ≥700

Ordering

Ordering

items

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1620100
100 g, -mr value of ≤0.15, recommended for all standard immunoelectrophoresis applications
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1620102
500 g, -mr value of ≤0.15, recommended for all standard immunoelectrophoresis applications
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(Discontinued) High -mr Agarose
1620001
50 g, -mr value of ≥0.25 for counter-immunoelectrophoresis