Certified™ Genetic Quality Tested (GQT) DNA Grade Agarose

Print

Overview


Specifications
DNA Agaroses Electro-endosmosis –mr Gel Strength (g/cm2) Gelation Temperature Sulfate Content Enzyme Inhibition Test DNase, RNase DNA Binding Gel Background Staining
Molecular biology agarose ≤0.12 ≥3,200 (1.5% gel) 36°C ≤0.12% Pass None None Very low
PCR agarose ≤0.12 ≥4,200 (4% gel) 40°C ≤0.11% Pass None None Very low
Low range ultra agarose ≤0.12 ≥1,500 (3% gel) 35°C ≤0.11% Pass None None Very low
Megabase agarose ≤0.12 ≥3,200 (1.5% gel) 36°C ≤0.12% Pass None None Very low
Low-melt agarose ≤0.12 ≥250 (1% gel) 26°C ≤0.10% Pass None None Very low
PCR-low-melt agarose ≤0.10 ≥1,000 (4% gel) 35°C ≤0.12% Pass None None Very low
Pulsed field Certified agarose ≤0.15 ≥2,500 (1.5% gel) 38°C ≤0.35% Pass None None Very low

Side by side agarose gel images
Certified Agarose Selection Guide
Application Molecular Biology Agarose PCR Agarose Low Range Ultra Agarose Low-Melt Agarose PCR Low-Melt Agarose Megabase Agarose Pulsed Field Agarose
Analytical Separation              
≥1,000 bp          
≤1,000 bp          
10–200 bp            
1 kb–2 Mb          
1 kb–5 Mb            
Preparative Separation              
Quantum Prep methods      
Low-melt methods          
Agarose methods          
In-gel applications          
Other Applications              
Postpreparative enzymatic treatments    
Tissue/cell culture            
Pulsed field sample preparation          
Blotting      


All Bio-Rad Certified Agarose products are guaranteed to be free of inhibitors, DNases, and RNases.

Certified Molecular Biology Agarose — a linear polymer with a very high molecular weight, which results in a gel structure unlike those of traditional agaroses. This general-purpose agarose ensures that DNA recovered from a preparative gel can be manipulated without compromising quality. With a very low sulfate content, this general-purpose agarose produces a gel with a very high gel strength and higher exclusion limit. The high electrophoretic mobility increases resolution and reduces run time, and the gels are easy to handle even at low agarose percentages. Analytical separation is ≥1,000 bp.

Certified PCR Agarose — recommended for separation of DNA fragments ≤1,000 bp. This high-strength agarose forms gels that are easy to handle and that remain flexible even at high gel percentages, which minimizes the risk of cracking or breaking. PCR agarose, unlike GQT products with similar sieving properties that gel at higher temperatures, gels at 40°C so it is faster and easier to prepare.

Certified Low-Range Ultra Agarose — provides superior resolution of small PCR fragments and primers at lower concentrations than standard agaroses. A 3% gel clearly resolves a 10 bp ladder, and a 4% gel approaches the resolution of an 8% polyacrylamide gel. Analytical separation is 20–800 bp.

Certified Megabase Agarose — the superior choice for CHEF and FIGE applications, with a very high gel strength, high electrophoretic mobility, and high exclusion limit. The gels remain easy to handle at concentrations as low as 0.3% and allow shorter run times. The separation range is between 1 kb and 5 Mb. Low background staining also provides superior imaging of high molecular weight DNA.

Certified Low-Melt Agarose — low-melting-temperature agarose with a high resolving capacity for DNA fragments ≥1,000 bp. It is recommended for preparative electrophoresis and for in-gel applications such as digestion, ligation, PCR, transformation, and sequencing. It is also recommended for embedding chromosomes and megabase-sized DNA for pulsed field applications.

Certified PCR Low-Melt Agarose — has a high sieving capacity and yields excellent resolution of fragments ≤1,000 bp in a low-melt or preparative format. This agarose is ideal for digestion by agarase and for all in-gel applications.

Pulsed-Field Certified Agarose — enables excellent separation and resolution of large DNA fragments in pulsed field gel applications. The optimal separation range is 1 kb–2 Mb. Although gels cast with this agarose have longer PFGE run times than Certified Megabase Agarose, running conditions for this agarose are a preset selectable method in the CHEF Mapper XA System.

Account price failed to display. Please try reloading the page. Reload Page

If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at   or send an
email to 

Certified Molecular Biology Agarose

1613100
25 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases

List Price:
 
$60.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Certified Molecular Biology Agarose

1613101
125 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases

List Price:
 
$175.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Certified Molecular Biology Agarose

1613102
500 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases

List Price:
 
$560.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Certified PCR Agarose

1613103
25 g, agarose for separation of DNA fragments ≤1,000 bp, high gel strength, certified free of inhibitors, DNases, RNases

List Price:
 
$135.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Certified PCR Agarose

1613104
125 g, agarose for separation of DNA fragments ≤1,000 bp, high gel strength, certified free of inhibitors, DNases, RNases

List Price:
 
$340.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Certified PCR Agarose

1613105
500 g, agarose for separation of DNA fragments ≤1,000 bp, high gel strength, certified free of inhibitors, DNases, RNases

List Price:
 
$1,430.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Certified Low Range Ultra Agarose

1613106
25 g, agarose for superior resolution of small PCR fragments and primers at low concentrations, certified free of inhibitors, DNases, RNases

List Price:
 
$140.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Certified Low Range Ultra Agarose

1613107
125 g, agarose for superior resolution of small PCR fragments and primers at low concentrations, certified free of inhibitors, DNases, RNases

List Price:
 
$430.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Certified Megabase Agarose

1613108
25 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases

List Price:
 
$87.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Certified Megabase Agarose

1613109
125 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases

List Price:
 
$285.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Certified Megabase Agarose

1613110
500 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases

List Price:
 
$900.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Certified Low-Melt Agarose

1613111
25 g, recommended for DNA fragments ≥1,000 kb, high sieving capacity low-melting temperature agarose, certified free of inhibitors, DNases, RNases

List Price:
 
$165.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Certified Low-Melt Agarose

1613112
125 g, recommended for DNA fragments ≥1,000 kb, high sieving capacity low-melting temperature agarose, certified free of inhibitors, DNases, RNases

List Price:
 
$475.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Certified PCR Low-Melt Agarose

1613113
25 g, recommended for fragments ≤1,000 bp, high sieving capacity low-melting temperature agarose, certified free of inhibitors, DNases, RNases

List Price:
 
$145.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Certified PCR Low-Melt Agarose

1613114
125 g, recommended for fragments ≤1,000 bp, high sieving capacity low-melting temperature agarose, certified free of inhibitors, DNases, RNases

List Price:
 
$420.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Certified PCR Low-Melt Agarose

1613115
500 g, recommended for fragments ≤1,000 bp, high sieving capacity low-melting temperature agarose, certified free of inhibitors, DNases, RNases

List Price:
 
$1,580.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Pulsed Field Certified Agarose

1620137
100 g, agarose for separation of large DNA in pulsed field gel applications, certified free of inhibitors, DNases, RNases

List Price:
 
$225.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Pulsed Field Certified Agarose

1620138
500 g, agarose for separation of large DNA in pulsed field gel applications, certified free of inhibitors, DNases, RNases

List Price:
 
$1,015.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Number Description Options
5458 Gene Expression Workflow Brochure, Rev B Click to download
5552 DNA Analysis Workflow Brochure, Rev B Click to download
2755 Certified Agarose Powder Brochure, Rev A Click to download