Certified Genetic Quality Tested (GQT) DNA Grade Agarose

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Overview


Specifications
DNA Agaroses Electro-endosmosis –mr Gel Strength (g/cm2) Gelation Temperature Sulfate Content Enzyme Inhibition Test DNase, RNase DNA Binding Gel Background Staining
Molecular biology agarose ≤0.12 ≥3,200 (1.5% gel) 36°C ≤0.12% Pass None None Very low
PCR agarose ≤0.12 ≥4,200 (4% gel) 40°C ≤0.11% Pass None None Very low
Low range ultra agarose ≤0.12 ≥1,500 (3% gel) 35°C ≤0.11% Pass None None Very low
Megabase agarose ≤0.12 ≥3,200 (1.5% gel) 36°C ≤0.12% Pass None None Very low
Low-melt agarose ≤0.12 ≥250 (1% gel) 26°C ≤0.10% Pass None None Very low
PCR-low-melt agarose ≤0.10 ≥1,000 (4% gel) 35°C ≤0.12% Pass None None Very low
Pulsed field Certified agarose ≤0.15 ≥2,500 (1.5% gel) 38°C ≤0.35% Pass None None Very low

Side by side agarose gel images
Certified Agarose Selection Guide
Application Molecular Biology Agarose PCR Agarose Low Range Ultra Agarose Low-Melt Agarose PCR Low-Melt Agarose Megabase Agarose Pulsed Field Agarose
Analytical Separation              
≥1,000 bp          
≤1,000 bp          
10–200 bp            
1 kb–2 Mb          
1 kb–5 Mb            
Preparative Separation              
Quantum Prep methods      
Low-melt methods          
Agarose methods          
In-gel applications          
Other Applications              
Postpreparative enzymatic treatments    
Tissue/cell culture            
Pulsed field sample preparation          
Blotting      


All Bio-Rad Certified agarose products are guaranteed to be free of inhibitors, DNases, and RNases.

Certified molecular biology agarose — This is a linear polymer with a very high molecular weight, which results in a gel structure unlike those of traditional agaroses. This general-purpose agarose ensures that DNA recovered from a preparative gel can be manipulated without compromising quality. With a very low sulfate content, this general-purpose agarose produces a gel with a very high gel strength and higher exclusion limit. The high electrophoretic mobility increases resolution and reduces run time, and the gels are easy to handle even at low agarose percentages. Analytical separation is ≥1,000 bp.

Certified PCR agarose — Certified PCR agarose is recommended for separation of DNA fragments ≤1,000 bp. This high-strength agarose forms gels that are easy to handle and that remain flexible even at high gel percentages, which minimizes the risk of cracking or breaking. PCR agarose, unlike GQT products with similar sieving properties that gel at higher temperatures, gels at 40°C so it is faster and easier to prepare.

Certified low range ultra agarose — This agarose provides superior resolution of small PCR fragments and primers at lower concentrations than standard agaroses. A 3% gel clearly resolves a 10 bp ladder, and a 4% gel approaches the resolution of an 8% polyacrylamide gel. Analytical separation is 20–800 bp.

Certified megabase agarose — With a very high gel strength, high electrophoretic mobility, and high exclusion limit, Certified megabase agarose is the superior choice for CHEF and FIGE applications. The gels remain easy to handle at concentrations as low as 0.3% and allow shorter run times. The separation range is between 1 kb and 5 Mb. Low background staining also provides superior imaging of high molecular weight DNA.

Certified low-melt agarose — This low-melting-temperature agarose has a high resolving capacity for DNA fragments ≥1,000 kb. It is recommended for preparative electrophoresis and for in-gel applications such as digestion, ligation, PCR, transformation, and sequencing. It is also recommended for embedding chromosomes and megabase-sized DNA for pulsed field applications.

Certified PCR low-melt agarose — This agarose has a high sieving capacity and yields excellent resolution of fragments ≤1,000 bp in a low-melt or preparative format. This agarose is ideal for digestion by agarose and for all in-gel applications.

Pulsed field Certified agarose — This agarose enables excellent separation and resolution of large DNA fragments in pulsed field gel applications. The optimal separation range is 1 kb–2 Mb. Although gels cast with this agarose have longer PFGE run times than Certified megabase agarose, running conditions for this agarose are a preset selectable method of the CHEF Mapper XA system auto-algorithm.

Certified Molecular Biology Agarose

161-3100
25 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases

List Price:   $59.00
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Certified Molecular Biology Agarose

161-3101
125 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases

List Price:   $169.00
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Certified Molecular Biology Agarose

161-3102
500 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases

List Price:   $543.00
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Certified PCR Agarose

161-3103
25 g, agarose for separation of DNA fragments ≤1,000 bp, high gel strength, certified free of inhibitors, DNases, RNases

List Price:   $128.00
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Certified PCR Agarose

161-3104
125 g, agarose for separation of DNA fragments ≤1,000 bp, high gel strength, certified free of inhibitors, DNases, RNases

List Price:   $328.00
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Certified PCR Agarose

161-3105
500 g, agarose for separation of DNA fragments ≤1,000 bp, high gel strength, certified free of inhibitors, DNases, RNases

List Price:   $1,388.00
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Certified Low Range Ultra Agarose

161-3106
25 g, agarose for superior resolution of small PCR fragments and primers at low concentrations, certified free of inhibitors, DNases, RNases

List Price:   $133.00
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Certified Low Range Ultra Agarose

161-3107
125 g, agarose for superior resolution of small PCR fragments and primers at low concentrations, certified free of inhibitors, DNases, RNases

List Price:   $415.00
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Certified Megabase Agarose

161-3108
25 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases

List Price:   $85.00
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Certified Megabase Agarose

161-3109
125 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases

List Price:   $276.00
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Certified Megabase Agarose

161-3110
500 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases

List Price:   $871.00
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Certified Low-Melt Agarose

161-3111
25 g, recommended for DNA fragments ≥1,000 kb, high sieving capacity low-melting temperature agarose, certified free of inhibitors, DNases, RNases

List Price:   $158.00
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Certified Low-Melt Agarose

161-3112
125 g, recommended for DNA fragments ≥1,000 kb, high sieving capacity low-melting temperature agarose, certified free of inhibitors, DNases, RNases

List Price:   $461.00
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Certified PCR Low-Melt Agarose

161-3113
25 g, recommended for fragments ≤1,000 bp, high sieving capacity low-melting temperature agarose, certified free of inhibitors, DNases, RNases

List Price:   $138.00
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Certified PCR Low-Melt Agarose

161-3114
125 g, recommended for fragments ≤1,000 bp, high sieving capacity low-melting temperature agarose, certified free of inhibitors, DNases, RNases

List Price:   $404.00
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Certified PCR Low-Melt Agarose

161-3115
500 g, recommended for fragments ≤1,000 bp, high sieving capacity low-melting temperature agarose, certified free of inhibitors, DNases, RNases

List Price:   $1,532.00
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Pulsed Field Certified Agarose

162-0137
100 g, agarose for separation of large DNA in pulsed field gel applications, certified free of inhibitors, DNases, RNases

List Price:   $215.00
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Pulsed Field Certified Agarose

162-0138
500 g, agarose for separation of large DNA in pulsed field gel applications, certified free of inhibitors, DNases, RNases

List Price:   $984.00
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Number Description Options
5458 Gene Expression Workflow Brochure, Rev B Click to download
5552 DNA Analysis Workflow Brochure, Rev B Click to download
2755 Certified Agarose Powder Brochure, Rev A Click to download