Silver Stain Plus Kit

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Silver Stain Plus Kit

Overview

Silver staining is a rapid and highly sensitive method for detecting proteins and nucleic acids in polyacrylamide gels. Bio-Rad offers kits and solutions for silver staining.
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Description

Silver staining is a highly sensitive method for detecting proteins and nucleic acids in polyacrylamide slab gels. Bio-Rad silver stain kits are 10–50-fold more sensitive than Coomassie Brilliant Blue R-250 for proteins (detection is ~0.1 ng/mm2) and 2–5-fold more sensitive than ethidium bromide for single and double-stranded DNA and RNA.

Silver Stain Plus™ stain is the most sensitive and easiest to use silver stain. The Silver Stain Plus kit is derived from the method developed by Gottlieb and Chavko (1987). The chemistry has been modified for both protein and nucleic acid staining in polyacrylamide and agarose gels. The Silver Stain Plus kit can detect as little as 0.6 ng of protein and DNA and requires only three steps, allowing visualization of your sample in less than 1 hour.

Reference

Gottlieb M and Chavko M (1987). Silver staining of native and denatured eukaryotic DNA in agarose gels. Anal Biochem 165, 33-37.


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Silver Stain Plus Kit

1610449
Kit for DNA or protein silver staining, includes 50 g development accelerator, 1 L fixative enhancer, 100 ml silver complex, 100 ml reduction moderator, 100 ml image developer, 1 empty 1 L bottle; enough for 13 full-size gels or 40 mini gels

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$264.00 
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Development Accelerator Reagent

1610448
50 g, replacement component of the Silver Stain Plus kit (#161-0449)

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$29.00 
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Fixative Enhancer Concentrate

1610461
1 L, replacement component of the Silver Stain Plus kit (#161-0449)

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$70.00 
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Silver Complex Solution

1610462
100 ml, replacement component of the Silver Stain Plus kit (#161-0449)

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$33.00 
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Reduction Moderator Solution

1610463
100 ml, replacement component of the Silver Stain Plus kit (#161-0449)

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$49.00 
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Image Development Reagent

1610464
100 ml, replacement component of the Silver Stain Plus kit (#161-0449)

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$65.00 
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Documents

Number Description Options
LIT442 Instruction Manual, Silver Stain Plus™, Rev E Click to download

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