Silver staining is a highly sensitive method for detecting proteins and nucleic acids in polyacrylamide slab gels. Bio-Rad silver stain kits are 10–50-fold more sensitive than Coomassie Brilliant Blue R-250 for proteins (detection is ~0.1 ng/mm2) and 2–5-fold more sensitive than ethidium bromide for single and double-stranded DNA and RNA.
Silver Stain Plus™ stain is the most sensitive and easiest to use silver stain. The Silver Stain Plus kit is derived from the method developed by Gottlieb and Chavko (1987). The chemistry has been modified for both protein and nucleic acid staining in polyacrylamide and agarose gels. The Silver Stain Plus kit can detect as little as 0.6 ng of protein and DNA and requires only three steps, allowing visualization of your sample in less than 1 hour.
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