Silver Stain Plus Kit

Silver Stain Plus Kit

Silver staining is a rapid and highly sensitive method for detecting proteins and nucleic acids in polyacrylamide gels. Bio-Rad offers kits and solutions for silver staining.

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Overview

Silver staining is a highly sensitive method for detecting proteins and nucleic acids in polyacrylamide slab gels. Bio-Rad silver stain kits are 10–50-fold more sensitive than Coomassie Brilliant Blue R-250 for proteins (detection is ~0.1 ng/mm2) and 2–5-fold more sensitive than ethidium bromide for single and double-stranded DNA and RNA.

Silver Stain Plus™ stain is the most sensitive and easiest to use silver stain. The Silver Stain Plus kit is derived from the method developed by Gottlieb and Chavko (1987). The chemistry has been modified for both protein and nucleic acid staining in polyacrylamide and agarose gels. The Silver Stain Plus kit can detect as little as 0.6 ng of protein and DNA and requires only three steps, allowing visualization of your sample in less than 1 hour.

Reference

Gottlieb M and Chavko M (1987). Silver staining of native and denatured eukaryotic DNA in agarose gels. Anal Biochem 165, 33-37.

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1610449
Kit for DNA or protein silver staining, includes 50 g development accelerator, 1 L fixative enhancer, 100 ml silver complex, 100 ml reduction moderator, 100 ml image developer, 1 empty 1 L bottle; enough for 13 full-size gels or 40 mini gels

Documents

Number Description Options
LIT442
Instruction Manual, Silver Stain Plus™, Rev E