Bio-Gel P Polyacrylamide Gels

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Overview

Specifications
  Hydrated Bead Size (µm) Hydrated Bed Volume Mass Dry Gel Typical Flow Rate (cm/hr)* Molecular Weight Fractionation Range
Bio-Gel P–2, fine 45–90 3 ml/g 5–10 100–1,800
Bio-Gel P–2, extra fine <45   <10  
Bio-Gel P–4, medium 90–180 4 ml/g 15–20 800–4,000
Bio-Gel P–4, fine 45–90   10–15  
Bio-Gel P–4, extra fine <45   <10  
Bio-Gel P–6, medium 90–180 6.5 ml/g 15–20 1,000–6,000
Bio-Gel P–6, fine 45–90   10–15  
Bio-Gel P–6, extra fine <45   <10  
Bio-Gel P–6DG gel 90–180   15–20  
Bio-Gel P–10 gel, medium 90–180 7.5 ml/g 15–20 1,500–20,000
Bio-Gel P–10 gel, fine 45–90   10–15  
Bio-Gel P–30 gel, medium 90–180 9 ml/g 7–13 2,400–40,000
Bio-Gel P–30 gel, fine 45–90   6–11  
Bio-Gel P–60 gel, medium 90–180 11 ml/g 4–6 3,000–60,000
Bio-Gel P–60 gel, fine 45–90   3–5  
Bio-Gel P–100 gel, medium 90–180 12 ml/g 4–6 5,000–100,000
Bio-Gel P–100 gel, fine 45–90   3–5  
 

*Flow rates determined in a 1.5 x 70 cm column using hydrostatic pressure head-to-bed ratio of 1:1

Bio-Gel P Properties
  Hydrated Bead Size (µm) Hydrated Bed Volume Mass Dry Gel Typical Flow Rate (cm/hr)* Molecular Weight Fractionation Range
Bio-Gel P–2, fine 45–90 3 ml/g 5–10 100–1,800
Bio-Gel P–2, extra fine <45   <10  
Bio-Gel P–4, medium 90–180 4 ml/g 15–20 800–4,000
Bio-Gel P–4, fine 45–90   10–15  
Bio-Gel P–4, extra fine <45   <10  
Bio-Gel P–6, medium 90–180 6.5 ml/g 15–20 1,000–6,000
Bio-Gel P–6, fine 45–90   10–15  
Bio-Gel P–6, extra fine <45   <10  
Bio-Gel P–6DG gel 90–180   15–20  
Bio-Gel P–10 gel, medium 90–180 7.5 ml/g 15–20 1,500–20,000
Bio-Gel P–10 gel, fine 45–90   10–15  
Bio-Gel P–30 gel, medium 90–180 9 ml/g 7–13 2,400–40,000
Bio-Gel P–30 gel, fine 45–90   6–11  
Bio-Gel P–60 gel, medium 90–180 11 ml/g 4–6 3,000–60,000
Bio-Gel P–60 gel, fine 45–90   3–5  
Bio-Gel P–100 gel, medium 90–180 12 ml/g 4–6 5,000–100,000
Bio-Gel P–100 gel, fine 45–90   3–5  

* Row rates determined in a 1.5 x 70 cm column using a hydrostatic pressure head-to-bed ratio of 1:1.

Bio-Gel P Polyacrylamide Gels
Bio-Gel P polyacrylamide gels are available as bottled media or prepacked cartridges.
Size Exclusion Information

Size Exclusion Chromatography

Size exclusion chromatography, also known as gel filtration chromatography, separates molecules by size. The gel matrix consists of spherical beads with pores of a specific size distribution. Separation occurs when molecules of different sizes are excluded from the pores. As small molecules diffuse into the pores, their flow through the column is retarded, while large molecules bypass the pores and are eluted in the column’s void volume. Consequently, molecules are separated according to size and elute in order of decreasing molecular weight.

Operating conditions and selection of media depend on the application and the desired resolution. A common method used in size exclusion chromatography is group separation, which includes desalting and fractionation.
Desalting — The molecule of interest is eluted in the void volume, while smaller molecules are retained in the gel pores; to achieve the desired separation, the matrix should have an exclusion limit smaller than the molecule of interest.
Fractionation — Molecules with a range of molecular weights are separated within the gel matrix; with this method, the molecules of interest should fall within the fractionation range of the gel.

Common applications include protein fractionation and molecular weight determination, nucleic acid separation, plasmid purification, and polysaccharide fractionation.

Resolution depends on particle size, pore size, flow rate, column dimensions, and sample volume. Generally, the highest resolution is obtained with low flow rates (2–10 cm/hr), long, narrow columns, small-particle-size gels, small sample volumes (1–5% of the total bed volume), 2-fold difference in molecular weights, and a sample viscosity that is the same as the eluent. For desalting, the sample volume can be as much as 30–40% of the total bed volume, and shorter, wider columns may be used.

For a list of antibody purification applications, request bulletin 1801. Protein applications are given in bulletin 1802.

Bio-Gel P Polyacrylamide Gels

Bio-Gel P polyacrylamide gels, for high-resolution gel filtration, are prepared by copolymerization of acrylamide and N,N'-methylene-bis-acrylamide. Bio-Gel P gels:

  • Are available in several particle size ranges with molecular weight exclusion limits ranging from 100 to 100,000
  • Are extremely hydrophilic and essentially nonionic
  • Provide efficient, gentle gel filtration of sensitive compounds
  • Do not support microbial growth or leach carbohydrates (due to their synthetic composition), as dextrose and agarose gels can

Bio-Gel P gels are autoclavable at pH 5.5–6.5 and operate over a pH range of 2–10 at room temperature. Flow rate and resolution increase with increasing temperature in the range of 4–80°C.

The gels are compatible with dilute organic acids, 8 M urea, chaotropic agents, detergents, and miscible organic solvents. Alcohol, up to 20% v/v, can be used to enhance the solubility of nucleotides, peptides, and tannins without altering the exclusion properties of the gels. Formamide may also be used at full strength since Bio-Gel P gels are completely swollen in this solvent.

Bio-Gel P-6DG gel, with a molecular weight exclusion limit of 6,000, is recommended specifically for desalting and buffer exchange applications. It provides rapid results with high sample recovery and minimal sample dilution. Its hydrophilicity ensures little or no interaction between the gel and sample molecules.

For more information, request bulletin 2068.

Properties
 
Hydrated Bead
Size (µm)
Hydrated Bed
Volume Mass Dry Gel
Typical Flow
Rates (cm/hr)*
Molecular Weight
Fractionation Range
Bio-Gel P-2 gel, fine
Bio-Gel P-2 gel, extra fine
45–90
<45
3 ml/g
5–10
<10
100–1,800
Bio-Gel P-4 gel, medium
Bio-Gel P-4 gel, fine
Bio-Gel P-4 gel, extra fine
90–180
45–90
<45
4 ml/g
15–20
10–15
<10
800–4,000
Bio-Gel P-6 gel, medium
Bio-Gel P-6 gel, fine
Bio-Gel P-6 gel, extra fine
Bio-Gel P-6DG gel
90–180
45–90
<45
90–180
6.5 ml/g
15–20
10–15
<10
15–20
1,000–6,000
Bio-Gel P-10 gel, medium
Bio-Gel P-10 gel, fine
90–180
45–90
7.5 ml/g
15–20
10–15
1,500–20,000
Bio-Gel P-30 gel, medium
Bio-Gel P-30 gel, fine
90–180
45–90
9 ml/g
7–13
6–11
2,500–40,000
Bio-Gel P-60 gel, medium
Bio-Gel P-60 gel, fine
90–180
45–90
11 ml/g
4–6
3–5
3,000–60,000
Bio-Gel P-100 gel, medium
Bio-Gel P-100 gel, fine
90–180
45–90
12 ml/g
4–6
3–5
50,000–100,000
* Flow rates determined in a 1.5 x 70 cm column using a hydrostatic pressure head-to-bed ratio of 1:1.



Bio-Gel P polyacrylamide gels, for high-resolution gel filtration, are prepared by copolymerization of acrylamide and N,N'-methylene-bis-acrylamide. Bio-Gel P gels:

  • Are available in several particle size ranges with molecular weight exclusion limits ranging from 100 to 100,000
  • Are extremely hydrophilic and essentially nonionic
  • Provide efficient, gentle gel filtration of sensitive compounds
  • Do not support microbial growth or leach carbohydrates (due to their synthetic composition) as dextrose and agarose gels can

Bio-Gel P gels are autoclavable at pH 5.5–6.5 and operate over a pH range of 2–10 at room temperature. Flow rate and resolution increase with increasing temperature in the range of 4–80°C.

The gels are compatible with dilute organic acids, 8 M urea, chaotropic agents, detergents, and miscible organic solvents. Alcohol, up to 20% v/v, can be used to enhance the solubility of nucleotides, peptides, and tannins without altering the exclusion properties of the gels. Formamide may also be used at full strength since Bio-Gel P gels are completely swollen in this solvent.

Bio-Gel P-6DG gel, with a molecular weight exclusion limit of 6,000, is recommended specifically for desalting and buffer exchange applications. It provides rapid results with high sample recovery and minimal sample dilution. Its hydrophilicity ensures little or no interaction between the gel and sample molecules.

For more information, request bulletin 2068.

Bio-Gel P-2 Gel

150-4114
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 100–1,800 MW fractionation range

List Price:   $276.00
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Bio-Gel P-2 Gel

150-4115
500 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 100–1,800 MW fractionation range

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Bio-Gel P-2 Gel

150-4118
100 g, extra fine polyacrylamide beads for size exclusion chromatography, <45 µm wet bead size, 100–1,800 MW fractionation range

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Bio-Gel P-4 Gel

150-4120
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 800–4,000 MW fractionation range

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Bio-Gel P-4 Gel

150-4124
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 800–4,000 MW fractionation range

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Bio-Gel P-4 Gel

150-4128
100 g, extra fine polyacrylamide beads for size exclusion chromatography, <45 µm wet bead size, 800–4,000 MW fractionation range

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Bio-Gel P-6 Gel

150-4130
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 1,000–6,000 MW fractionation range

List Price:   $240.00
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Bio-Gel P-6 Gel

150-4134
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 1,000–6,000 MW fractionation range

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Bio-Gel P-6DG Gel

150-0738
100 g, polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 1,000–6,000 MW fractionation range

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Bio-Gel P-6DG Gel

150-0739
1 kg, fine polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 1,000–6,000 MW fractionation range

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Bio-Gel P-10 Gel

150-4140
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 1,500–20,000 MW fractionation range

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Bio-Gel P-10 Gel

150-4144
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 1,500–20,000 MW fractionation range

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Bio-Gel P-30 Gel

150-4150
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 2,500–40,000 MW fractionation range

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Bio-Gel P-30 Gel

150-4154
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 2,500–40,000 MW fractionation range

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Bio-Gel P-60 Gel

150-4160
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 3,000–60,000 MW fractionation range

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Bio-Gel P-60 Gel

150-4164
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 3,000–60,000 MW fractionation range

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Bio-Gel P-100 Gel

150-4170
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 5,000–100,000 MW fractionation range

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Bio-Gel P-100 Gel

150-4174
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 5,000–100,000 MW fractionation range

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Number Description Options
LIT174 Instruction Manual, Bio-Gel P Polyacrylamide Gel, Rev B Click to download [ Add to Cart (Free) ]
LIT547 Notice for Extra Fine P-Gel Users Click to download
2068 Desalting With Bio-Gel P-6DG Desalting Gel, Rev B Click to download