Econo-Pac® 10DG Desalting Prepacked Gravity Flow Columns

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Econo-Pac 10DG Desalting Pre-Packed Gravity Flow Columns

Overview

Specifications
Product
Description
Packed
Support
Bed
Volume
Particle Size (µm) Capacity Applications
Econo-Pac®
protein A
column
Affi-Gel®
protein A gel
2 ml 10–14 mg
mouse IgG1*
10–14 mg
mouse IgG1*
Gravity-flow purification of monoclonal antibodies from small amounts of ascites fluid or serum.
Econo-Pac
protein A kit
300mg mouse
IgG1*
Contains everything necessary to purify monoclonal anitbodies from ascites fluid or serum, including Affi-Gel protein A column, reusable Econo-Pac 10DG columns (for a sample preparation and buffer exchange of the purified antibody), and MAPS II buffers. Enough reagents to purify 500 mg mouse IgG1.
Econo-Pac
serum IgG
purification
column
DEAE Affi-Gel
blue gel
10 ml 75–150 3 ml serum per column per run For purification of IgG from other serum proteins and plasminogen with only residual transferrin contamination. Columns can be resued 8–10 times.
Econo-Pac
serum IgG
purification
3 ml serum per column Contains everything necessary to purify IgG from serum, including 5 Econo-Pac serum IgG purification columns, reusable Econo-Pac 10DG column for sample preparation, and premixed buffers for human and rabbit IgG applications. Up to 15 ml serum can be purified at one time.
Econo-Pac
10DG desalting gel column
Bio-Gel® P-6 gel 10 ml 90–180 3 ml sample
per column per run
Gravity-flow column for desalting and buffer exchange applications. Exclusion limit is 6,000 Da.

* When used with MAPS II buffers.

Prepacked Cartridge Selection by Application
Cartridge by Application Support Type of Separation Chemical Form Protein capacity/ml*
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange -N+(CH3)3 ≥37 mg BSA
UNOsphere Q UNOsphere™ Q Strong anion exchange -N+(CH3)3 180 mg BSA
UNOsphere S UNOsphere S Strong cation exchange -SO3- 60 mg human IgG
DEAE Macro-Prep DEAE Weak anion exchange -HN+(C2H5)2 >35 mg protein
Protein Purification
High S Macro-Prep High S Strong cation exchange -SO3- ≥49 mg human IgG
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity Cibacron Blue
F3GA and DEAE
0.2–1.0 ml serum
Antibody Purification
Protein A Affi-Prep® protein A Affinity Protein A 8–10 mg mouse monoclonal IgG1
CHT™ Type I CHT ceramic
hydroxyapatite Type I
Hydroxyapatite (Ca5(PO4)3OH)2 16–23 mg human IgG
CHT Type II CHT ceramic
hydroxyapatite Type II
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human IgG
Affinity-Tagged Protein Purification
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
His-tagged protein
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV***
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 mlm serum
11 mg albumin

*   Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
**  IMAC and GST cartridges are designed for use with the Profinia protein purification system.
*** Column volume.

Column and Resin Selection Guide

Column and Resin Selection Guide Capture Column and Resin Selection Guide Intermediate Purification Column and Resin Selection Guide Polishing

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Econo-Pac 10DG Desalting Columns for gravity-flow chromatography allow fast and simplified antibody purification. These columns are prepacked with Bio-Gel® P-6DG Gel and include an upper frit, a snap-off end tip, graduated column markings, and a 30 ml total column volume. To improve column performance at higher flow rates, use an Econo-Pac Flow Adaptor (738-0019).

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Econo-Pac 10DG Desalting Columns

7322010
Pkg of 30, 10 ml packed resin, 20 ml reservoir, prepacked gravity-flow chromatography column, includes upper frit

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Econo-Pac Flow Adaptor

7380019
Pkg of 1, 1.5 cm column ID, for use with Econo-Pac chromatography columns

1 7380019 /templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/738-0019_0921120612326.xml 7380019 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jb6po1ym 2017-11-14 07:08:06 true true internet/product_SKU jb6po1ym templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/738-0019_0921120612326.xml LSR 2019-05-04 20:55:44 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998748372 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:55:44 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/738-0019_0921120612326.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/738-0019_0921120612326.xml 7380019-econo-pac-flow-adaptor 7380019 Econo-Pac Flow Adaptor Econo-Pac Flow Adaptor Pkg of 1, 1.5 cm column ID, for use with Econo-Pac chromatography columns Pkg of 1, 1.5 cm column ID, for use with Econo-Column chromatography columns Pkg of 1, 1.5 cm column ID, for use with Econo-Pac chromatography columns /webroot/web/images/ps/products/affinity_media/sku_thumb/global/738-0019_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/738-0019_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/738-0019_feature.jpg preview ,VN RepEx 738-0019 Pkg of 1, 1.5 cm column ID, for use with Econo-Column chromatography columns 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Instrument-Systems--Components N 0 <p>Use the Econo-Pac flow adaptor to improve column performance by eliminating head space above the gel bed and protecting it from disruption during sample loading.</p> /webroot/web/html/lsr/products/SKUs/autopopulated 738-0019.html /webroot/web/html/lsr/products/SKUs/autopopulated/738-0019.html false $161.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7380019&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7380019&CartQuote=QT&quantity= 1241 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7380019&AddToDefaultWishList=true&quantity= 41115700 161.00 Product detail page 7380019
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Flow Adaptor Maintenance Kit

7380025
Maintenance kit for 1.5 cm Econo-Column flow adaptor, includes 10 bed supports and 5 O-rings

7380025 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/738-0025_0921120643495.xml 7380025 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jo7tzfq4 2017-11-14 07:07:57 true true internet/product_SKU jo7tzfq4 templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/738-0025_0921120643495.xml LSR 2019-05-04 20:56:06 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555997277368 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:56:06 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/738-0025_0921120643495.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/738-0025_0921120643495.xml 7380025-flow-adaptor-maintenance-kit 7380025 Flow Adaptor Maintenance Kit Flow Adaptor Maintenance Kit Maintenance kit for 1.5 cm Econo-Column flow adaptor, includes 10 bed supports and 5 O-rings Maintenance kit for 1.5 cm Econo-Column flow adaptor, includes cam mechanism, 10 bed supports and 5 O-rings Maintenance kit for 1.5 cm Econo-Column flow adaptor, includes 10 bed supports and 5 O-rings /webroot/web/images/lsr/products/chromatography/sku_thumb/global/738-0025_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/738-0025_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/738-0025_feature.jpg preview ,VN RepEx 738-0025 Maintenance kit for 1.5 cm Econo-Column flow adaptor, includes 10 bed supports and 5 O-rings 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Columns N 0 false $64.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7380025&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7380025&CartQuote=QT&quantity= 1241 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7380025&AddToDefaultWishList=true&quantity= 41115700 64.00 Product detail page 7380025
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LIT81 Instruction Manual, Econo-Pac® 10DG Columns Click to download

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