Econo-Pac® Protein A Prepacked Gravity Flow Columns

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Econo-Pac Protein A Pre-Packed Gravity Flow Columns

Overview

Specifications
Product
Description
Packed
Support
Bed
Volume
Particle Size (µm) Capacity Applications
Econo-Pac®
protein A
column
Affi-Gel®
protein A gel
2 ml 10–14 mg
mouse IgG1*
10–14 mg
mouse IgG1*
Gravity-flow purification of monoclonal antibodies from small amounts of ascites fluid or serum.
Econo-Pac
protein A kit
300mg mouse
IgG1*
Contains everything necessary to purify monoclonal anitbodies from ascites fluid or serum, including Affi-Gel protein A column, reusable Econo-Pac 10DG columns (for a sample preparation and buffer exchange of the purified antibody), and MAPS II buffers. Enough reagents to purify 500 mg mouse IgG1.
Econo-Pac
serum IgG
purification
column
DEAE Affi-Gel
blue gel
10 ml 75–150 3 ml serum per column per run For purification of IgG from other serum proteins and plasminogen with only residual transferrin contamination. Columns can be resued 8–10 times.
Econo-Pac
serum IgG
purification
3 ml serum per column Contains everything necessary to purify IgG from serum, including 5 Econo-Pac serum IgG purification columns, reusable Econo-Pac 10DG column for sample preparation, and premixed buffers for human and rabbit IgG applications. Up to 15 ml serum can be purified at one time.
Econo-Pac
10DG desalting gel column
Bio-Gel® P-6 gel 10 ml 90–180 3 ml sample
per column per run
Gravity-flow column for desalting and buffer exchange applications. Exclusion limit is 6,000 Da.

* When used with MAPS II buffers.

Column and Resin Selection Guide

Column and Resin Selection Guide Capture Column and Resin Selection Guide Intermediate Purification Column and Resin Selection Guide Polishing

Affinity Chromatography Resin Selection Guide
  Matrix Functional Group Specificity Capacity Working pH Pressure Limit Applications
Ready-to-Use Affinity Media
Nuvia™ IMAC High capacity, pressure-stable polymer based on UNOsphere™ beads NTA charged with Ni2+ Histidine ≥40 mg/ml* 2–14 45 psi
(3.1 bar)
Purification of recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity™ IMAC Pressure-stable polymer based on UNOsphere beads IDA, available charged with Ni2+ and uncharged Histidine ≥15 mg/ml* 1–14 100 psi
(6.8 bar)
Suitable for purification of larger and difficult-to-purify recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity GST** Pressure-stable polymer based on UNOsphere beads Immobilized glutathione GST-tagged proteins ≥10 mg/ml 1–14 45 psi
(3.1 bar)
Purification of recombinant GST-tagged proteins
Profinity eXact™*** Crosslinked 6% agarose Subtilisin protease Subtilisin prodomain ≥3 mg/ml tag-free protein 2–10 10 psi (minus system pressure) Generation of native, tag-free proteins by on-column purification and cleavage
Affi-Gel® Protein A Crosslinked agarose Protein A
2 mg/ml
IgG   2–10 15 psi
(1 bar)
Purification of IgG from ascites, serum, and culture fluid; with MAPS buffer system, purification of 10 mg mouse IgG per ml of gel is possible
Affi-Prep® Protein A Pressure-stable polymer Protein A
2 mg/ml
IgG   2–10 1,000 psi (70 bar) Purified IgG from ascites, serum, and culture fluid; pressure-stable support for process-scale applications
Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA 1.9 mg/ml Albumin; general ≥11 mg/ml 2–10 15 psi
(1 bar)
Binds many nucleotide-requiring enzymes, albumin, and other proteins
DEAE Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and DEAE Albumin and serum proteins 0.14 ml serum/ml gel 2–10 15 psi
(1 bar)
Purifies protease-free IgG from ascites, serum, and culture fluid with minimal sample preparation
CM Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and CM Albumin and serum proteins 0.17–0.5 ml serum/ml gel 2–11 15 psi
(1 bar)
Produces albumin- and protease-free antibody preparation from serum without prior dialysis
Affi-Prep Polymyxin Pressure-stable polymer Polymyxin 2–4 mg/ml Endotoxins >5 mg/ml 2–10 1,000 psi
(70 bar)
Endotoxin removal
Affi-Gel Boronate Polyacrylamide gel Boronate 1.05 ± 0.15 cis-diols

meq/g
130 µmol sorbitol/ml 2–10 15 psi
(1 bar)
Adsorption of cis-hydroxyl–containing molecules, including sugars, nucleotides, and glycopeptides
Activated Media for Spontaneous Ligand Immobilization
Profinity Epoxide Pressure-stable polymer based on UNOsphere beads Epoxy group Nucleophiles; amini, thiol, -COOH 36–40 mg/ml lgG 1–14 Up to 80 psi
(5.5 bar)
Activated matrix for the immobilization of various ligands (for example, protein A, StrepTactin, and immunoglobulins)
Affi-Gel 10 Crosslinked agarose N-hydroxy-succinimide ≥10 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)
For coupling proteins with pI 6.5–11
Affi-Gel 15 Crosslinked agarose N-hydroxy-succinimide ≥9 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)
For coupling proteins with pI 6.5–11
Affi-Gel Hz Crosslinked agarose Hydrazide Oxidized carbohydrates 1–5 mg/ml 2–10 15 psi
(1 bar)
Immobilization of immunoglobulins and other glycoproteins via carbohydrate molecules
Affinity Media Using Carbodiimide Activation
Affi-Gel 102 Crosslinked agarose -NH2 16 ± 4 meq/ml -COOH 40 mg 2–11 15 psi
(1 bar)
Carbodiimide coupling of carboxyl-containing ligand

* Refer to Bulletin 3193 for purification conditions.
** For example, Profinity GST Resin is available only in prepacked cartridges.
***Profinity eXact Purification Resin.

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Econo-Pac Protein A Prepacked Columns for gravity-flow chromatography allow fast and simplified antibody purification. These prepacked Econo-Pac columns are filled with Affi-Gel® Protein A Media and include an upper frit, a snap-off end tip, graduated column markings, and a 30 ml total column volume. To improve column performance at higher flow rates, use an Econo-Pac Flow Adaptor (738-0019).

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Econo-Pac Protein A Columns

7322022
Pkg of 5, prepacked gravity-flow chromatography columns, prefilled with Affi-Gel protein A gel

0 7322022 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-2022_0921120637443.xml 7322022 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jb6po1z5 2017-11-14 07:07:55 true true internet/product_SKU jb6po1z5 templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-2022_0921120637443.xml LSR 2019-05-04 20:56:03 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555997271942 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:56:03 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-2022_0921120637443.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-2022_0921120637443.xml 7322022-econo-pac-protein-columns 7322022 Econo-Pac Protein A Columns Econo-Pac Protein A Columns Pkg of 5, prepacked gravity-flow chromatography columns, prefilled with Affi-Gel protein A gel Pkg of 5, prepacked gravity-flow chromatography columns, prefilled with Affi-Gel protein A gel Pkg of 5, prepacked gravity-flow chromatography columns, prefilled with Affi-Gel protein A gel /webroot/web/images/ps/products/affinity_media/sku_thumb/global/732-2022_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/732-2022_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/732-2022_feature.jpg RepEx 732-2022 Pkg of 5, prepacked gravity-flow chromatography columns, prefilled with Affi-Gel protein A gel 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Columns N 0 false $666.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7322022&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7322022&CartQuote=QT&quantity= 7653 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7322022&AddToDefaultWishList=true&quantity= 41106500 666.00 Product detail page 7322022
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Econo-Pac® Protein A Kit

7322020
Purification kit, includes Affi-Gel® protein A column, four 10DG desalting columns, buffers

1 7322020 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-2020_0921120637502.xml 7322020 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jb6po1zl 2017-11-14 07:07:55 true true internet/product_SKU jb6po1zl templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-2020_0921120637502.xml LSR 2019-05-04 20:56:07 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555997271900 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:56:07 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-2020_0921120637502.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-2020_0921120637502.xml 7322020-econo-pac-protein-kit 7322020 Econo-Pac Protein A Kit Econo-Pac<sup>&reg;</sup> Protein A Kit Purification kit, includes Affi-Gel<sup>&reg;</sup> protein A column, four 10DG desalting columns, buffers Pkg of 5, prepacked gravity-flow chromatography columns, prefilled with Affi-Gel protein A gel Purification kit, includes Affi-Gel<sup>&reg;</sup> protein A column, four 10DG desalting columns, buffers /webroot/web/images/ps/products/affinity_media/sku_thumb/global/732-2020_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/732-2020_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/732-2020_feature.jpg RepEx 732-2020 Pkg of 5, prepacked gravity-flow chromatography columns, prefilled with Affi-Gel protein A gel 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Columns N 0 ,VN / false $397.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7322020&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7322020&CartQuote=QT&quantity= 7653 true true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7322020&AddToDefaultWishList=true&quantity= 41106500 397.00 Product detail page 7322020
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Hazardous  
(Discontinued) Econo-Pac Serum IgG Purification Column

7322026
Pkg of 5, prepacked gravity-flow chromatography columns, prefilled with DEAE Affi-Gel blue gel

This product is no longer available. Please contact technical support for more information on replacement product.

The following replacement is recommended:

  • DEAE Affi-Gel Blue Gel (1537307)
  • Econo-Pac® Chromatography Columns, Pkg of 50 (7321010)
(Discontinued) Econo-Pac Serum IgG Purification Kit

7322027
Antibody purification kit, includes 5 x 5 ml DEAE Affi-Gel Blue columns, 1 x 10 ml 10DG column, buffers

This product is no longer available. Please contact technical support for more information on replacement product.

The following replacement is recommended:

  • DEAE Affi-Gel Blue Gel (1537307)
  • Econo-Pac 10DG Desalting Columns (7322010)
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Econo-Pac Flow Adaptor

7380019
Pkg of 1, 1.5 cm column ID, for use with Econo-Pac chromatography columns

3 7380019 /templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/738-0019_0921120612326.xml 7380019 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jb6po1ym 2017-11-14 07:08:06 true true internet/product_SKU jb6po1ym templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/738-0019_0921120612326.xml LSR 2019-05-04 20:55:44 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998748372 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:55:44 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/738-0019_0921120612326.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/738-0019_0921120612326.xml 7380019-econo-pac-flow-adaptor 7380019 Econo-Pac Flow Adaptor Econo-Pac Flow Adaptor Pkg of 1, 1.5 cm column ID, for use with Econo-Pac chromatography columns Pkg of 1, 1.5 cm column ID, for use with Econo-Column chromatography columns Pkg of 1, 1.5 cm column ID, for use with Econo-Pac chromatography columns /webroot/web/images/ps/products/affinity_media/sku_thumb/global/738-0019_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/738-0019_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/738-0019_feature.jpg preview ,VN RepEx 738-0019 Pkg of 1, 1.5 cm column ID, for use with Econo-Column chromatography columns 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Instrument-Systems--Components N 0 <p>Use the Econo-Pac flow adaptor to improve column performance by eliminating head space above the gel bed and protecting it from disruption during sample loading.</p> /webroot/web/html/lsr/products/SKUs/autopopulated 738-0019.html /webroot/web/html/lsr/products/SKUs/autopopulated/738-0019.html false $161.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7380019&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7380019&CartQuote=QT&quantity= 1241 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7380019&AddToDefaultWishList=true&quantity= 41115700 161.00 Product detail page 7380019
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Number Description Options
LIT154 Instruction Manual, Econo-Pac® Serum IgG Purification Kit and Econo-Pac Serum IgG Purification Columns, Rev B Click to download
LIT153 Instruction Manual, Econo-Pac® Protein A Kit, Protein A Columns, Rev B Click to download

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