UNOsphere SUPrA™ Media

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Overview

Prepacked Chromatography Cartridge Selection Guide
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
5 x 1 ml 1 x 5 ml 5 x 5 ml
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange –N+(CH3)3 ≥37 mg BSA 732–4120 732–4122 732–4124
UNOsphere™ Q NOsphereQ Strong anion exchange –N+(CH3)3 180 mg BSA 732–4100 732–4102 732–4104
UNOsphere S UNOsphere S Strong cation exchange –SO3- 60 mg human lgG 732–4110 732–4112 732–4114
DEAE Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 >35 mg protein 732–4140 732–4142 732–4144
Protein Purification
High S Macro-Prep High S Strong cation exchange –SO3- ≥49 mg human lgG 732–4130 732–4132 732–4134
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

732–4632 732–4634
Antibody Purification
Protein A Affi-Prep® protein A Affinity Recombinant protein A 30 mg/ml human IgG
732–4200, 1 x 1 ml 732–4201, 5 x 1 ml 732–4202, 1 x 5 ml
Protein A Affi-Prep® protein A Affinity Protein A 8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
732–4600 732–4602
CHT™ Type I CHT ceramic
hydroxyapatite Type I
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human lgG
732–4322 732–4324
CHT Type II CHT ceramic
hydroxyapatite
Type II, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human lgG
732–4332 732–4334
Affinity-Tagged Protein Purification
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
Histidine-tagged protein
732–4610 732–4612 732–4614
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
732–4620 732–4622 732–4624
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 ml serum
11 mg albumin
732–4642 732–4644

Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
1 x 5 ml 5 x 5 ml 5 x 10 ml 1 x 50 ml 5 x 50 ml
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV*** 732–
4502
732–
4504
732–
5304
732–
5312
732–
5314

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.

** IMAC and GST cartridges are designed for use with the Profinia protein purification system.

*** Column volume.

PDF Bulletin 5584 Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

Monoclonal Antibody Purification Workflow
Monoclonal Antibody Purification Workflow

Workflow for mAb purification with UNOsphere SUPra affinity, UNOsphere Q and CHT type I media.

PDF Bulletin 5728 To read more click here

Flow Performance of UNOsphere SUPrA Media

Narrow elution profile of antibody on UNOsphere SUPrA media.

UNOsphere SUPrA affinity chromatography support is based on recombinant protein A and is designed for process-scale purification of monoclonal antibodies. The protein A ligand is produced in E. coli without the use of material from animal origin.

The UNOsphere™ base bead is a macroporous polymeric bead that is designed for robust and scaleable process applications. The outstanding flow-pressure performance of UNOsphere chromatography media allows their use in large-scale process applications.

UNOsphere SUPrA affinity chromatography supports come with full regulatory support and are backed by the support of Bio-Rad’s global application and development team.

If you are interested in ordering large-volume quantities, please contact your local Bio-Rad representative to discuss your requirements.

Composition Highly cross-linked polymer
Particle size range 53–61 µm
Ligand Recombinant protein A
Coupling chemistry Epoxy
Working pH range 3–11
Dynamic binding capacity* 30 ±3 mg/ml at 150 cm/hr
25 ±2 mg/ml at 300 cm/hr
20 ±2 mg/ml at 450 cm/hr
(Minimum spec: 20 mg/ml at 300 cm/hr)
Chemical stability 10 mM hydrochloric acid
6 M guanidine hydrochloride
0.1 M arginine (pH 2.8)
0.1 M citrate (pH 2.8)
0.1 M glycine (pH 2.8)
Cleaning-in-place (CIP) solutions 6 M guanidine hydrochloride
10 mM hydrochloric acid
0.1 M sodium hydroxide
1 M acetic acid/20% ethanol
Recommended mobile phase velocity range 100–600 cm/hr
Temperature stability 4–40°C
Delivery conditions 50% slurry in 20% ethanol
Storage conditions 4–8°C
* 10% breakthrough capacity determined with 1.0 mg/ml polyclonal human IgG in 1.1 x 10 cm column.  
UNOsphere SUPrA Affinity Chromatography Media

156-0218
25 ml, protein A affinity chromatography media

List Price:   $834.00
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UNOsphere SUPrA Affinity Chromatography Media

156-0219
100 ml, protein A affinity chromatography media

List Price:   $1,947.00
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UNOsphere SUPrA Affinity Chromatography Media

156-0220
500 ml, protein A affinity chromatography media

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UNOsphere SUPrA Affinity Chromatography Media

156-0221
5 L, protein A affinity chromatography media

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UNOsphere SUPrA Affinity Chromatography Media

156-0222
10 L, protein A affinity chromatography media

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Number Description Options
5925 Affinity Purification Buffer Kit Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]