Affi-Gel Protein A Resin

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Overview

Chromatography on Affi-Gel protein A support yields highly purified immunoglobulins (IgG), selectively removes IgG from serum, or adsorbs immune complexes for antigen purification.
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Description

Description

Chromatography on Affi-Gel protein A media yields highly purified immunoglobulins (IgGs), selectively removes IgG prior to analysis of other immunoglobulin classes, or adsorbs immune complexes for antigen purification. Protein A binds to the Fc region of immunoglobulins, especially IgG from mammalian species.

Features and Benefits

  • High purity of IgGs
  • High affinity for mammalian IgG
  • High capacities for mouse IgG1 as well as other subclasses with MAPS optimized buffer

With Affi-Gel Protein A Media and MAPS II buffers, you can purify up to 10 mg of IgG1/ml of gel. This is 8–10 times higher than is possible with standard methods. In addition, the MAPS process and Affi-Gel protein A media permit greater binding of mouse IgG1 than do immobilized protein G media.

Ordering

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1536153
5 ml, ready-to-use protein A crosslinked agarose support
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1536154
50 ml, ready-to-use protein A crosslinked agarose support
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1536159
Ready-to-use affinity media kit, includes 5 ml Affi-Gel protein A support, Affi-Gel Protein A MAPS II buffers, 1 x 10 cm Econo-Column column; for 500 mg of mouse IgG1
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1536161
Optimized buffer concentrate, for use with Affi-Gel and Affi-Prep protein A media, makes 5 L

Kits & Reagents

Kits & Reagents

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7322022
Pkg of 5, prepacked gravity-flow chromatography columns, prefilled with Affi-Gel protein A gel
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7322020
Purification kit, includes Affi-Gel® protein A column, four 10DG desalting columns, buffers
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7322010
Pkg of 30, 10 ml packed resin, 20 ml reservoir, prepacked gravity-flow chromatography column, includes upper frit
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(Discontinued) Poly Column Rack
7317005
Pkg of 1, 20-place rack, with removable tube rack, for use with Econo-Pac® or Poly-Prep® Chromatography Columns
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7380019
Pkg of 1, 1.5 cm column ID, for use with Econo-Pac chromatography columns

Documents

Documents

Number Description Options
5334
A Method for Rapid, Large-Scale Removal of Albumin and IgG From Human Serum Using the BioLogic DuoFlow Chromatography System, Rev A
LIT88
Instruction Manual, Affi-Gel® Protein A MAPS II Kit
LIT153
Instruction Manual, Econo-Pac® Protein A Kit, Protein A Columns, Rev B