RAPID'Sakazakii Medium

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Overview

Key Benefits
  • Improved formulation results in big easy-to-read colored colonies
  • Short protocol eliminates the need for secondary enrichment
  • Results a full day earlier provides a time and cost savings
  • Ability to detect atypical strains
  • Good correlation with ISO method
Protocol
Detection of Chronobacter spp.
Validation

Validated and Adapted for Food Detection

AFNOR
BRD 07/22-05/12
ALTERNATIVE ANALYTICAL METHODS FOR AGRIBUSINESS
Certified by AFNOR Certification
www.afnor-validation.com

The RAPID’Sakazakii Medium protocol is certified by AFNOR Certification, according to the ISO 16140 requirements as an alternative method to the ISO/TS 22964 reference standard, for the detection of Cronobacter spp. in infant formula (No. BRD: 07/22-05/12).

Nordval Validation Ref No032.



Intended Use

RAPID'Sakazakii Medium is a selective chromogenic agar that is intended for the isolation and detection of Cronobacter spp. (formerly classified as Enterobacter sakazakii). The most common source of Cronobacter spp. contamination is powdered infant formula.

Key Benefits

  • Easy-to-read chromogenic reaction
    • Blue to blue-green colonies
  • Cost-effective short protocol
    • No selective enrichment in mLST + Vancomycin
    • Negative results in less than 42 hr
  • Validation
    • Certified NF VALIDATION according to the ISO 16140:2003 standard for the detection of Cronobacterspp. in infant milk powders

Principle

Detection is based on a specific enzymatic activity common to all species of Cronobacter. Hydrolysis of the chromogenic substrate 5-bromo-4-chloro-3-indol α-D-glucopyranoside leads to the formation of typical turquoise-colored colonies.

The medium includes compounds carefully selected to promote the growth of Cronobacter spp. while suppressing interfering flora, giving the medium increased selectivity. Competing bacteria, if not completely inhibited, will form light purple or gray colonies.

RAPID'Sakazakii Chromogenic Assay

Enzymatic Activity
Cronobacter
spp.

Other
Enterobacteriaceae
α-D-Glucosidase + -
     

Simple and Rapid Protocol

RAPID'Sakazakii Medium is formulated for use in standard methods (for example, the ISO 22964 reference method). The medium can also be used with a shortened protocol to test “clean” samples such as powdered infant formula with a low level of contamination. The short protocol is used to detect Cronobacter spp. in 24 hr after an 18-hr enrichment in buffered peptone water, eliminating the need for selective enrichment.

Ordering Information

RAPID'Sakazakii Agar is offered as ready-to-use 90 mm plates (3563971) or as a dehydrated agar base (3564976)

Assay type Chromogenic media
Organism Cronobacter, Enterobacter sakazakii
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RAPID'sakazakii Agar #356-3971
RAPID'Sakazakii Agar Plates

3563971
Pkg of 20, 90 mm agar plates

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$80.00
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RAPID'Sakazakii Agar
RAPID'Sakazakii Agar, dehydrated

3564976
Pkg of 1, 500 g of dehydrated agar base, enough for 13.6 L of medium

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$852.00
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Buffered Peptone Water Broth, ready to use

3554179
Pkg of 6, 225 ml bottles

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$32.00
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Buffered Peptone Water Broth, dehydrated

3564684
Pkg of 1, 500 g

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$72.00
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iQ-Check® Cronobacter PCR Detection Kit

3578137
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

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Number Description Options
FSD_16288 RAPID’Chromogenic Media Brochure, Rev L Click to download
PF_158 RAPID’Chromogenic Methoden für die Lebensmittel- und Umweltanalytik, Rev C (Deutsch) Click to download
PF_159 Métodos RAPID’Chromogenic para Análisis de Alimentos y Medioambiente, Rev C (Español) Click to download
PF_160 Méthodes RAPID’Chromogenic pour les Tests Alimentaires et Environnementaux, Rev C (Français) Click to download
PF_161 Metodi RAPID’Chromogenic per Analisi Alimentari e Ambientali, Rev C (Italiano) Click to download
PF_162 Métodos RAPID’Cromogénicos para Testes Alimentares e Ambientais, Rev C (Português) Click to download
FSD_17933 Food Safety and Quality Diagnostics Booklet, Rev I Click to download
TS_Rsakaz_en RAPID’Sakazakii Agar Technical Sheet Click to download