UNOsphere SUPrA Resin

Image
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Overview

UNOsphere SUPrA affinity resin are designed for process-scale purification of monoclonal antibodies. These affinity resins are built on the proven UNOsphere matrix for predictable performance.
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Description

Description

UNOsphere SUPrA Affinity Chromatography Resin is based on recombinant protein A and is designed for process-scale antibody purification. The protein A ligand is produced in E. coli without the use of material of animal origin — ideal for the production of monoclonal antibodies.

UNOsphere Resins are based on macroporous polymeric beads designed for robust and scalable process applications. The outstanding flow-pressure performance of UNOsphere SUPrA Resins allows their use in large-scale process applications.

UNOsphere SUPrA Affinity Chromatography Resin comes with full regulatory support backed by Bio-Rad’s global application and development team.

More Information

Larger volumes and special packaging for industrial applications are available upon request. Please contact your local Bio-Rad representative to discuss your requirements.

Specifications

Specifications

Composition
Highly cross-linked polymer
Particle size range
53–61 µm
Ligand Recombinant
Protein A
Coupling chemistry
Epoxy
Working pH range
3–11
Dynamic binding capacity*
30 ± 3 mg/ml at 150 cm/hr
25 ± 2 mg/ml at 300 cm/hr
20 ± 2 mg/ml 450 cm/hr
(Minimum spec: 20 mg/ml at 300 cm/hr)
Chemical stability
10 mM hydrochloric acid
6 M guanidine hydrochloride
0.1 M arginine (pH 2.8)
0.1 M citrate (pH 2.8)
0.1 M glycine (pH 2.8)
Cleaning-In-Place (CIP) Solutions Cleaning-In-Place (CIP) Solutions
6 M guanidine hydrochloride
10 mM hydrochloric acid
0.1 M sodium hydroxide
1 M acetic acid/20% ethanol
Recommended mobile phase velocity range
100–600 cm/hr
Temperature stability
4–40°C
Delivery conditions
50% slurry in 20% ethanol
Storage conditions
4–8°C
*  10% breakthrough capacity determined with 1.0 mg/ml polyclonal human IgG in 1.1 x 10 cm column.
**No significant change in chromatographic performance
 

Ordering

Ordering

items

Use the filters below to refine results!

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7324709
2 x 96-well plates, 20 µl, filter plate prepacked with UNOsphere SUPrA affinity chromatography resin
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7324729
Pkg of 1, 1 ml, column prepacked with UNOsphere SUPrA Affinity Chromatography Resin
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7324749
Pkg of 1, 5 ml, column prepacked with UNOsphere SUPrA Affinity Chromatography Resin
foresight-unosphere-supra-robocolumn-unit-200-microl-732-4834-thumb.jpg
7324834
1 row of 8 columns, 200 µl, RoboColumn unit prepacked with UNOsphere SUPrA Affinity Chromatography Resin
foresight-unosphere-supra-robocolumn-unit-600-microl-732-4835-thumb.jpg
7324835
1 row of 8 columns, 600 µl, RoboColumn unit prepacked with UNOsphere SUPrA Affinity Chromatography Resin
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1560218
25 ml, protein A affinity chromatography media
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1560219
100 ml, protein A affinity chromatography media
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156-0220
500 ml, protein A affinity chromatography media
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156-0221
5 L, protein A affinity chromatography media
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156-0222
10 L, protein A affinity chromatography media

Kits & Reagents

Kits & Reagents

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7324200
1 ml, prepacked, UNOsphere SUPrA affinity media cartridge
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7324201
Pkg of 5, 1 ml, prepacked, UNOsphere SUPrA affinity media cartridge
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7324202
5 ml, prepacked, UNOsphere SUPrA affinity media cartridge
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7324408
UNOsphere SUPrA™ affinity cartridge, UNOsphere™ Q cartridge, CHT™ Type 1, 40 µm cartridge, 1 x 5 ml each

Documents

Documents

Number Description Options
6484
High-Performance Columns for Antibody and Tagged Protein Purifications Product Information Sheet, Rev B
[ Add to Cart (Free) ]
6053
Application of UNOsphere SUPrA Media, a New Protein A Affinity Chromatographic Support, for Both Laboratory-Scale and Industrial Antibody Purification, Rev A
5729
UNOsphere SUPrA Affinity Chromatography Media Product Information Sheet, Rev B
[ Add to Cart (Free) ]
5728
Monoclonal Antibody Purification Using UNOsphere SUPrA Media, Rev A
[ Add to Cart (Free) ]
10014997
Instruction Manual, Bio-Scale™ Mini UNOsphere SUPrA™ cartridges, 1 and 5 ml, Rev B
10014430
Instruction Manual, UNOsphere SUPrA™ Affinity Chromatography Media, Rev C
5662
Process Chromatography Applications Laboratory Brochure, Rev B
6875
Practical Guide: Selecting the Optimal Resins for Monoclonal Antibody Process Purification, Ver A
6790
Practical Guide: Selecting the Optimal Resins for Mammalian Virus Process Purification and Clearance, Ver A
6793
Practical Guide: Selecting the Optimal Resins for Immunoglobulin G (IgG) Process Purification, Ver A
6881
Practical Guide: Selecting the Optimal Resins for Removal of DNA Contamination during Process Purification, Ver A
6786
Monoclonal Antibody Purification Resin Selection Card, Ver A
6789
IgG Polyclonal Antibody Purification Resin Selection Card, Ver A
6889
Novel Selectivities and Workflows to Streamline mAb Purification Processes, Ver A
6867
UNOsphere™ Chromatography Resins Publications List, Ver A
6861
Monoclonal Antibody Purification: Capture Purification Resins Brochure, Ver A
6886
Quick Monoclonal Antibody Purification Strategy: Affinity-Based Capture Flier, Ver A