Mini-Sub Cell GT Cell

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Mini-Sub Cell GT Cell

Overview

Mini-Sub® Cell GT Complete Systems*
Catalog # Casting
Gates
Gel Caster UVTP Tray (cm) Combs PowerPac Basic
Power Supply
7 x 7** 7 x 10 8-Well 15-Well
170-4406      
170-4466        
170-4486    
170-4467      
164-0300    
170-4487              
164-0303            

* Mini-Sub cell systems purchased before 1996 (Mini-Sub DNA cell) require casting tray (catalog #170-4330). This tray is not compatible with the Mini-Sub cell GT system.
** Allows casting gels in the cell using casting gates; 7 x 10 gels can be cast with a gel caster.

Sub-Cell Selection Guide
 
  Mini-Sub Cell GT Wide Mini-Sub Cell GT Sub-Cell GT
Cell size,
cm (W x L x H)
9.2 x 25.5 x 5.6 17.8 x 25.5 x 6.8 18 x 40.5 x 9.4
Gel tray sizes,
cm (W x L)
7 x 7
7 x 10
15 x 7
15 x 10
15 x 10
15 x 15
15 x 20
15 x 25
ReadyAgarose gels accommodated Yes Yes No
Sample throughput 8–30* 10–60* 1–120**
Base buffer volume, L ~0.27 ~0.65 ~1.0
Distance to electrode, cm 14.5 14.5 29.5
Bromophenol blue dye migration rates, cm/hr (at 75 V) ~4.5 ~4.5 ~3.0
* Sample throughput value assumes 1–2 combs per gel.
** Sample throughput value assumes 1–4 combs per gel.
Mini-Sub Cell Comb Selection Guide
Catalog # # of Wells Height* Thickness, mm Width
of Well, mm
Length
of Teeth, mm
Volume, µl
(in 5 mm deep gel)
1704464 15 Fixed 0.75 3 10 11.25
1704465** 15 Fixed 1.5 3 10 22.5
1704332 15 Adjustable 1 2.6 10.2 13
1704462 8 Fixed 0.75 5.5 11 20
1704463** 8 Fixed 1.5 5.5 11 40
1704333 8 Adjustable 1 5.5 10.2 27.5
1704461 2 preparative Fixed 1.5 20 10 150
  2 reference     4   30
1704460 1 preparative Fixed 1.5 44 10 330
  2 reference     3   22.5

* Fixed-height combs must be used with GT gel trays. Adjustable-height combs require comb holder, catalog #1704331.
** Combs included in systems.

Agarose Gel Casting Guide

Hand Casting Options for a Variety of Gel Sizes

Two accessories are available for hand casting with the Sub-Cell family of electrophoresis cells: gel casters and casting gates. Gel casters offer the flexibility of accommodating several different-sized gel trays. These are available for the entire line of Sub-Cell systems. Casting gates, available in specific sizes for each cell, offer the convenience of casting directly in the Sub-Cell cells.



Gel Caster Guide
Tray Size (cm) Mini Gel Caster Full-Size Gel Caster Model 96 Gel Caster Model 192 Gel Caster
7 x 7
7 x 10
15 x 7
15 x 10
15 x 15  
15 x 20  
15 x 25  
25 x 10    
25 x 15    
25 x 20      
25 x 25      

Casting Guide
Electrophoresis Cell Tray Size (cm) Casting Gates Gel Caster
Mini-Sub cell GT 7 x 7
7 x 10  
Wide Mini-Sub cell GT 15 x 7
15 x 10  
Sub-Cell GT 15 x 10  
15 x 15
15 x 20  
15 x 25  
Sub-Cell Model 96 25 x 10
25 x 15  
Sub-Cell Model 192 25 x 10  
25 x 15
25 x 20  
25 x 25  

Horizontal mini cells offer speed, simplicity, and economy. The wide mini cells are best suited for multiple-sample and rapid-screening DNA electrophoresis applications.
Read full description

Ordering Information


  • Description
  • Specifications
  • Ordering
  • Accessories
  • Kits & Reagents
  • Combs
  • Precast gels
  • Documents

Description

The redesigned Mini-Sub Cell GT Cell offers updated features that make electrophoresis even easier. The Mini-Sub Cell GT Cell can resolve up to 30 samples; its short, narrow format allows 7 and 10 cm runs with speed, simplicity, and economy.

Bio-Rad's mini cells resolve EcoRI or HindIII digests of lambda DNA in only 1.5 hours at 60 V. Small DNA fragments can be separated in as little as 15 minutes at 150 V.

All mini cells accommodate ReadyAgarose Precast Gels to save time and allow highly reproducible separations, and include a buffer tank, a safety lid with cables, and a leveling bubble. System configurations that include additional accessories are also available (see the Mini-Sub Cell GT Complete Systems Selection Guide for more details).

Features:

  • QuickSnap* electrodes are easy to remove, simplifying cleaning
  • Arrow on the side of the base indicates the direction of the run and ensures proper orientation of the gel
  • Color-coded, labeled electrodes and labeled base guarantee correct positioning of the lid on the base
  • Tabs on the base permit easy removal of the lid, reducing buffer spillage, and also prevent incorrect lid positioning
  • Reverse-compatible design allows the cells to be used with components from older models
  • Clear plastic construction for easy sample visualization
  • UV-transparent gel trays with fluorescent ruler**
  • Gel-casting gates to cast your own gels right in the cell, or optional caster for tape-free casting**
  • Combs to fit every need (fixed-height drop-in combs, adjustable-height combs, and preparative combs)**

* Patent pending.
** Purchase separately for ReadySub-Cell GT systems.

Mini ReadySub-Cell GT Cells

The mini ReadySub-Cell GT cell is identical to the Mini-Sub Cell GT Cell, except it is dedicated to running ReadyAgarose precast gels. These gels lock securely into the mini ReadySub-Cell chamber, offering convenience and reproducibility. This cell does not include casting gates, tray, or combs. Kits are available to upgrade ReadySub-Cell GT Cells for handcasting capability.


Specifications

Cell size (W x L x H) 9.2 x 25.5 x 5.6 cm
Gel tray sizes (OD) (W x L) 7 x 7 cm
7 x 10 cm
ReadyAgarose gels accommodated Yes, 8-, 12-, 2 x 8-well
Sample throughput 8–30
Base buffer volume ~270 ml
Buffer recirculation No
Bromophenol blue migration ~4.5 cm/hr (at 75 V)

Ordering

3128 Casting Gates Gel Caster Power Supply 3151 7 x 7 7 x 10

21 items

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UV Transparent Tray Size, cm (W x L)

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Mini-Sub Cell GT Horizontal Electrophoresis System, 7 x 7 cm tray, with casting gates

1704406
Mini horizontal electrophoresis system, includes 8- and 15-well combs, 7 x 7 cm UV-transparent tray, casting gates

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$454.00 
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Mini-Sub Cell GT Horizontal Electrophoresis System, 7 x 10 cm tray

1704466
Mini horizontal electrophoresis system, includes 8- and 15-well combs, 7 x 10 cm UV-transparent tray

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$447.00 
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Mini-Sub Cell GT Horizontal Electrophoresis System, 7 x 7 cm tray, with casting gates and mini-gel caster

1704486
Mini horizontal electrophoresis system, includes 8- and 15-well combs, 7 x 7 cm UV-transparent tray, casting gates, mini-gel caster

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$540.00 
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Mini-Sub Cell GT Horizontal Electrophoresis System, 7 x 10 cm tray, with mini-gel caster

1704467
Mini horizontal electrophoresis system, includes 8- and 15-well combs, 7 x 10 cm (W x L) UV-transparent tray, mini-gel caster

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$540.00 
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Mini-Sub Cell GT Horizontal Electrophoresis System and PowerPac Basic Power Supply

1640300
Mini horizontal electrophoresis system, includes 8- and 15-well combs, gel caster, 7 x 10 cm (W x L) UV-transparent tray, basic power supply (1645050)

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$993.00 
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Mini ReadySub-Cell GT Horizontal Electrophoresis System

1704487
Horizontal electrophoresis cell, includes lid and electrodes, accommodates 8- and 12-well mini ReadyAgarose Gels

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$368.00 
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Mini ReadySub-Cell GT Horizontal Electrophoresis Cell and PowerPac Basic Power Supply

1640303
Horizontal electrophoresis cell system, accommodates 8- and 12-well mini ReadyAgarose Gels, includes basic power supply (1645050)

This product is being discontinued soon. Please contact customer service for more information.

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Accessories

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Mini-Sub Cell GT Mini Handcasting Kit

1704491
Agarose mini-gel casting kit, includes 7 x 7 cm gel tray, 8- and 15-well 1.5 mm fixed height combs, casting gates, allows casting gels in cells using casting gates, for use with Mini-Sub Cell GT systems

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$179.00 
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Mini Sub-Cell GT Gel Caster

1704422
Pkg of 1, gel caster for use with Mini-Sub Cell GT and Wide Mini-Sub Cell GT Systems

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$104.00 
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Mini-Sub Cell GT UV-Transparent Gel Tray, 7 x 7 cm

1704436
UV-Transparent Gel Tray, 7 x 7 cm, for Mini-Sub Cell GT Systems, allows casting gels in cells using casting gates, 2 slots for fixed-height combs

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$66.00 
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Mini-Sub Cell GT UV-Transparent Gel Tray, 7 x 10 cm

1704435
UV-Transparent Gel Tray, 7 x 10 cm, for Mini-Sub Cell GT Systems, 2 slots for fixed-height combs

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$65.00 
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Original UV-Transparent Mini-Gel Tray

1704330
Pkg of 1, 7 x 10 cm, horizontal agarose gel electrophoresis casting tray, for use with Mini-Sub cell systems purchased before 1996 (Mini-Sub DNA cell)

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$108.00 
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Mini-Sub Cell GT Casting Gates

1704434
Pkg of 2, horizontal agarose gel electrophoresis casting gates, for use with Mini-Sub cell GT systems

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$111.00 
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Mini-Comb Holder

1704331
Pkg of 1, horizontal electrophoresis comb holder, for use with Mini-Sub cell GT adjustable-height combs

This product is being discontinued soon. Please contact customer service for more information.

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Mini-Sub Cell GT Anode (Red), QuickSnap Electrode Assembly

1704362
Pkg of 1, electrode assembly for use with Mini-Sub cell GT systems, 2009 model

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$93.00 
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Mini-Sub Cell GT Cathode (Black), QuickSnap Electrode Assembly

1704363
Pkg of 1, electrode assembly for use with Mini-Sub cell GT systems, 2009 model

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$93.00 
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Kits & Reagents

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Certified Molecular Biology Agarose, 25 g

1613100
25 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases

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$70.00 
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Certified Molecular Biology Agarose, 125 g

1613101
125 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases

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$213.00 
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Certified Molecular Biology Agarose, 500 g

1613102
500 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases

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$689.00 
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Certified PCR Agarose, 25 g

1613103
25 g, agarose for separation of DNA fragments ≤1,000 bp, high gel strength, certified free of inhibitors, DNases, RNases

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$166.00 
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Certified PCR Agarose, 500 g

1613105
500 g, agarose for separation of DNA fragments ≤1,000 bp, high gel strength, certified free of inhibitors, DNases, RNases

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$1,745.00 
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Certified Low Range Ultra Agarose, 25 g

1613106
25 g, agarose for superior resolution of small PCR fragments and primers at low concentrations, certified free of inhibitors, DNases, RNases

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$173.00 
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Certified Low Range Ultra Agarose, 125 g

1613107
125 g, agarose for superior resolution of small PCR fragments and primers at low concentrations, certified free of inhibitors, DNases, RNases

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$527.00 
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Certified Low Melt Agarose, 25 g

1613111
25 g, recommended for DNA fragments ≥1,000 kb, high sieving capacity low-melting temperature agarose, certified free of inhibitors, DNases, RNases

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$200.00 
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Certified Low Melt Agarose, 125 g

1613112
125 g, recommended for DNA fragments ≥1,000 kb, high sieving capacity low-melting temperature agarose, certified free of inhibitors, DNases, RNases

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$580.00 
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Certified PCR Low Melt Agarose, 25g

1613113
25 g, recommended for fragments ≤1,000 bp, high sieving capacity low-melting temperature agarose, certified free of inhibitors, DNases, RNases

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$178.00 
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Certified PCR Low Melt Agarose, 125g

1613114
125 g, recommended for fragments ≤1,000 bp, high sieving capacity low-melting temperature agarose, certified free of inhibitors, DNases, RNases

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$517.00 
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Certified PCR Low Melt Agarose, 500g

1613115
500 g, recommended for fragments ≤1,000 bp, high sieving capacity low-melting temperature agarose, certified free of inhibitors, DNases, RNases

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$1,890.00 
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20 bp Molecular Ruler

1708201
250 μl, 0.2 μg/μl, DNA standard, 20–1,000 bp, 50 bands in 20 bp increments, 100 applications

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$138.00 
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EZ Load 20 bp Molecular Ruler

1708351
500 μl, 0.1 μg/μl, ready-to-load DNA standard, 20–1,000 bp, 50 bands, includes 1 ml 5x nucleic acid sample buffer, 100 applications

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$138.00 
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100 bp Molecular Ruler

1708202
250 μl, 0.1 μg/μl, DNA standard, 100–1,000 bp, 10 bands in 100 bp increments, 100 applications

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$138.00 
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EZ Load 100 bp Molecular Ruler

1708352
500 μl, 0.05 μg/μl, ready-to-load DNA standard, 100–1,000 bp, 10 bands, includes 1 ml 5x nucleic acid sample buffer, 100 applications

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$138.00 
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100 bp PCR Molecular Ruler

1708206
200 μl, 0.2 μg/μl, DNA standard, 100–3,000 bp, 30 bands in 100 bp increments, 100 applications

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$138.00 
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EZ Load 100 bp PCR Molecular Ruler

1708353
500 μl, 0.08 μg/μl, ready-to-load DNA standard, 100–3,000 bp, 30 bands, includes 1 ml 5x nucleic acid sample buffer, 100 applications

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$138.00 
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500 bp Molecular Ruler

1708203
200 μl, 0.2 μg/μl, DNA standard, 500–8,000 bp, 16 bands in 500 bp increments, 100 applications

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$138.00 
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EZ Load 500 bp Molecular Ruler

1708354
500 μl, 0.08 μg/μl, ready-to-load DNA standard, 500–8,000 bp, 16 bands, includes 1 ml 5x nucleic acid sample buffer, 100 applications

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$138.00 
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1 kb Molecular Ruler

1708204
200 μl, 0.2 μg/μl, DNA standard, 1–15 kb, 15 bands in 1 kb increments, 100 applications

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$109.00 
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EZ Load 1 kb Molecular Ruler

1708355
500 μl, 0.08 μg/μl, ready-to-load DNA standard, 1–15 kb, 15 bands, includes 1 ml 5x nucleic acid sample buffer, 100 applications

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$109.00 
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2.5 kb Molecular Ruler

1708205
400 μl, 0.1 μg/μl, DNA standard, 2.5–35 kb, 14 bands in 2.5 kb increments, 100 applications

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$195.00 
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AmpliSize Molecular Ruler

1708200
250 μl, 0.1 μg/μl, DNA standard at 50% GC content, 50–2,000 bp, 10 bands, 50 applications

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$195.00 
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(Discontinued) Precision Molecular Mass Ruler

1708207
250 μl, 0.1 μg/μl, DNA standard for quantitation in gels, 100–1,000 bp, 5 bands at 10 to 100 ng, 100 applications

This product is no longer available. Please contact technical support for more information on replacement product.

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EZ Load™ Precision Molecular Mass Ruler

1708356
500 μl, 0.05 μg/μl, ready-to-load DNA standard for quantitation in gels, 100–1,000 bp, 5 bands at 10 to 100 ng, 100 applications

This product is being discontinued soon. Please contact customer service for more information.

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10x TBE (Tris/Boric Acid/EDTA) Buffer, 1 L

1610733
1 L, TBE nucleic acid electrophoresis buffer solution, pH 8.3

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$43.00 
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10x TBE (Tris/Boric Acid/EDTA) Buffer, 5 L

1610770
5 L cube, TBE nucleic acid electrophoresis buffer solution, pH 8.3

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$132.00 
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(Discontinued) 10x Tris/Boric Acid/EDTA (TBE), Extended Range, Nucleic Acid Electrophoresis Buffer

1610741
1 L, premixed buffer for extended range DNA sequencing runs, pH 8.3

This product is no longer available. Please contact technical support for more information on replacement product.
50x TAE (Tris/Acetic Acid/EDTA) Buffer, 1 L

1610743
1 L, TAE nucleic acid electrophoresis buffer solution, pH 8.0

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$100.00 
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50x TAE (Tris/Acetic Acid/EDTA) Buffer, 5 L

1610773
5 L cube, TAE nucleic acid electrophoresis buffer solution, pH 8.0

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$294.00 
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Ethidium Bromide Solution, 10 mg/mL

1610433
10 mL ethidium bromide solution for fluorescent staining of nucleic acids, 10 mg/mL

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$46.00 
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Hazardous  
EDTA

1610729
500 g, ≥99% pure EDTA (disodium ethylenediaminetetraacetate dihydrate) powder

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$82.00 
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Tris

1610716
500 g, ≥99.8% pure Tris base (Tris[hydroxymethyl]aminomethane or Trimethamine)

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$37.00 
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Tris

1610719
1 kg, ≥99.8% pure Tris base (Tris[hydroxymethyl]aminomethane or Trimethamine)

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Combs

x

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If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at or send an
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15-Well Comb

1704464
Pkg of 1, 0.75 mm thick, fixed-height comb, for use with Mini-Sub cell GT systems, must use with GT gel tray

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$71.00 
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15-Well Comb

1704465
Pkg of 1, 1.5 mm thick, fixed-height comb, for use with Mini-Sub cell GT systems, must use with GT gel tray

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$67.00 
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15-Well Comb

1704332
Pkg of 1, 1.0 mm thick, adjustable-height comb, for use with Mini-Sub cell GT systems, requires comb holder (#170-4331)

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$71.00 
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8-Well Comb

1704462
Pkg of 1, 0.75 mm thick, fixed-height comb, for use with Mini-Sub cell GT systems, must use with GT gel tray

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$71.00 
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8-Well Comb

1704463
Pkg of 1, 1.5 mm thick, fixed-height comb, for use with Mini-Sub cell GT systems, must use with GT gel tray

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$67.00 
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8-Well Comb

1704333
Pkg of 1, 1.0 mm thick, adjustable-height comb, for use with Mini-Sub cell GT systems, requires comb holder (#170-4331)

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$71.00 
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Preparative Comb

1704461
Pkg of 1, 1.5 mm thick, includes 2 preparative and 2 reference wells, fixed height, for use with Mini-Sub cell GT systems, must use with GT gel tray

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$71.00 
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Preparative Comb

1704460
Pkg of 1, 1.5 mm thick, includes 1 preparative and 2 reference wells, fixed height, for use with Mini-Sub cell GT systems, must use with GT gel tray

List Price: 
 
$71.00 
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Preparative Comb

1704342
Pkg of 1, 3 mm thick, preparative comb with 1 preparative and 2 reference wells, adjustable height, requires comb holder (#170-4331)

This product is being discontinued soon. Please contact customer service for more information.

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1% TBE Mini ReadyAgarose Precast Gel, 7.1 x 10 cm, 8-well, with ethidium bromide

1613004
8-well precast mini gel, TBE, 1.0% agarose plus ethidium bromide, 7.1 x 10 cm (tray size)

List Price: 
 
$13.00 
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(Discontinued) 3% TBE Mini ReadyAgarose™ Precast Gel, 7.1 x 10 cm, 8-well, with ethidium bromide

1613006
8-well precast mini gel, TBE, 3.0% agarose plus ethidium bromide, 7.1 x 10 cm (tray size)

This product is no longer available. Please contact technical support for more information on replacement product.

The following replacement is recommended:

  • 1% TBE Mini ReadyAgarose Precast Gel, 7.1 x 10 cm, 8-well, with ethidium bromide (1613004)
1% TBE Mini ReadyAgarose Precast Gel, 7.1 x 10 cm, 12-well, with ethidium bromide

1613010
12-well precast mini gel, TBE, 1.0% agarose plus ethidium bromide, 7.1 x 10 cm (tray size)

List Price: 
 
$12.00 
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1% TAE Mini ReadyAgarose Precast Gel, 7.1 x 10 cm, 8-well

1613015
8-well precast mini gel, TAE, 1.0% agarose, 7.1 x 10 cm (tray size)

List Price: 
 
$12.00 
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1% TAE Mini ReadyAgarose Precast Gel, 7.1 x 10 cm, 8-well, with ethidium bromide

1613016
8-well precast mini gel, TAE, 1.0% agarose plus ethidium bromide, 7.1 x 10 cm (tray size)

List Price: 
 
$12.00 
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