iQ-Check S. Typhimurium PCR Detection Kit

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Overview

Rapid, flexible, and specific detection of Salmonella Typhimurium in food and environmental samples (including primary production environments).

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Description

Description

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The iQ-Check S. Typhimurium Real Time PCR Detection Kit provides rapid identification of DNA sequences specific to Salmonella Typhimurium in pork and poultry, and environmental samples. This test can be run in parallel with other iQ-Check Kits.

Intended Use

Though the incidence of human salmonellosis is decreasing in some areas, millions of cases are still reported worldwide each year. Salmonella enterica subspecies enterica serovar Typhimurium (Salmonella Typhimurium or ST) is one of the most frequently implicated serovars. Pork and poultry feed have been identified as among the most common food sources linked to these S. Typhimurium infections. Regulations have been implemented in the EU to monitor Salmonella spp. and specific serotypes, including S. Typhimurium, in poultry from the primary production stage to food products. The iQ-Check S. Typhimurium PCR Detection Kit permits the rapid and specific detection of S. Typhimurium in food and environmental samples (including primary production environments) within a few hours after microbiological enrichment. Up to 94 samples can be processed with this easy-to-use procedure.

Key Benefits

  • Simple and rapid — ready-to-use reagents, automated detection and interpretation of results
  • Flexible — 48-well or 96-well formats for high throughput; screen of up to 94 samples per run
  • Highly specific — optimized combination of primers and probes
  • Reliable — positive, negative and internal controls
  • Economical — can be run in parallel with other iQ-Check Tests
  • Fully integrated — use with Bio-Rad reagents and instruments, from the enrichment to confirmation with immune sera

Bio-Rad offers a complete solution with standardized reagents, including enrichment media, PCR kits, confirmation agars, immune sera and PCR detection systems, in addition to software for automated analysis specific to each test. The iQ-Check S. Typhimurium Real Time PCR Kit provides a tool along with the iQ-Check Salmonella II and iQ-Check S. Enteritidis Kits for fast, highly specific identification allowing you to take the right decision.

Kit Components

  • Fluorescent probes
  • PCR amplification mix
  • Positive and negative PCR controls

Related Products

Related Categories

Specifications

Specifications

Organism
Salmonella Typhimurium

Ordering

Ordering

items

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12004306

Pkg of 1 kit, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

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3594970

Pkg of 1, 1 vial of lyophilized iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial of iQ-Check 10x Activation Buffer (9 ml)

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3564684
Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions
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3554179
Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions
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3564684
Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions

Accessories

Accessories

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HSP9655XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design
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HSP9955XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, barcoded, 7-digit sequential, Code 128, rigid 2-component design

Equipment

Equipment

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3600037

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

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17007992

96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, cables

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12016658
17007991

96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, and cables

Documents

Documents

Number Description Options
10000065187
iQ-Check S. Typhimurium, User Guide (English, French, German, Italian, Portuguese, Spanish)
FSD_16439
iQ-Check Real-Time PCR Solution Brochure
3311
CFX Opus 96 Real-Time PCR System brochure
3309
CFX Opus 96 and CFX Opus Deepwell Real-Time PCR system product information sheet
7465
Performance summary, iQ-Check S. Enteritidis and iQ-Check S. Typhimurium
7039
Easy I Extraction Deep Well Protocol Using the Thermoshaker Quick Guide
3240
iQ-Check PCR Kits Easy I and II Extraction TUBE Protocol Quick Guide
6960
iQ-Check Prep Solution Brochure
FSD_17933
Food Safety and Quality Reference Guide
10000058391
iQ-Check Free DNA Removal Solution Product Insert
7322
iQ-Check Free DNA Removal Solution — Quick Guide
6834
iQ-Check Free DNA Removal Solution Brochure
10000119734
iQ-Check Purification Reagent Technical sheet
i3s2016
A Novel Simplex Real-Time PCR Assay for Rapid Molecular Detection and Typing of Salmonella Typhimurium
IAFP2016
Development of a Real-Time PCR Assay to Specifically Detect Salmonella Typhimurium
7274
Complete Solutions for Service Laboratories
10000128740
Buffered Peptone Water Plus, User Guide (English, French, German, Italian, Portuguese, Spanish, Chinese)
10000128738
Buffered Peptone Water Standard, User Guide (English, French, German, Italian, Portuguese, Spanish)
3276
CFX Manager Software (IDE) iQ-Check Prep sample setup Protocol, Quick Guide