Gene Pulser MXcell™ Electroporation System

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Overview

Gene Pulser MXcell electroporation systems are no longer available, but current users can order electroporation plates and cuvettes for their transfection applications.

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Description

Description

Discontinued Product
The Gene Pulser MXcell electroporation system is no longer available. However, current users can order electroporation plates and cuvettes for their transfection applications.

Existing Gene Pulser MXcell systems can be used to electroporate as few as 105 cells by using a 96-well plate or as many as 106 to 107 cells in a 12-well plate or in a cuvette.

 

Alternatives to the Gene Pulser MXcell system for meeting your transfection needs include:

  • The Gene Pulser Xcell™ systems — modular electroporation systems for transfecting every cell type. They include a main unit, a ShockPod™ cuvette chamber, and your choice of accessory modules: the capacitance extender (CE) module and the pulse controller (PC) module
  • Lipid transfection reagents — provide optimal transfection efficiencies for nucleic acid delivery into mammalian cells. General use and RNAi-optimized transfection reagents are available

Specifications

Specifications

Gene Pulser Xcell™ Total SystemIncludes main unit, CE module, PC module, and ShockPod™ cuvette chamber
 
Waveform
Exponential or square
Voltage, V
10–3,000
Capacitance, µF
10–500 V: 25–3,275 µF in 25 µF increments; 500–3,000 V: 10, 25, 50 µF
Resistance (parallel), Ω
50–1,000 Ω in 50 Ω increments, plus infinity
Sample resistance, Ω
20 Ω minimum at 10–2,500 V; 600 Ω minimum at 2,500–3,000 V
Square-wave timing
10–500 V: 0.05–10 ms in 0.05 ms increments, 10–100 ms pulse in 1 ms increments, 1–10 pulses, 0.1–10 sec interval; 500–3,000 V: 0.05–5 ms in 0.05 ms increments, 1–2 pulses, 5 sec minimum interval
Dimensions (W x D x H), cm
Main unit: 31 x 30 x 14
CE module: 31 x 28 x 9
PC module: 31 x 28 x 5
Weight, kg
Main unit: 6.6
CE module: 3.1
PC module: 1.9
Gene Pulser Xcell Eukaryotic System Includes main unit, CE module, and ShockPod cuvette chamber
 
Waveform
Exponential or square
Voltage, V
10–3,000
Capacitance, µF
10–500 V: 25–3,275 µF in 25 µF increments; 500–3,000 V: 10, 25, 50 µF
Sample resistance, Ω
20 Ω minimum at 10–2,500 V; 600 Ω minimum at 2,500–3,000 V
Square-wave timing
10–500 V: 0.05–10 ms in 0.05 ms increments, 10–100 ms pulse in 1 ms increments, 1–10 pulses, 0.1–10 sec interval; 500–3,000 V: 0.05–5 ms in 0.05 ms increments, 1–2 pulses, 5 sec minimum interval
Dimensions (W x D x H), cm
Main unit: 31 x 30 x 14
CE module: 31 x 28 x 9
Weight, kg
Main unit: 6.6
CE module: 3.1
Gene Pulser Xcell Microbial System Includes main unit, PC module, and ShockPod cuvette chamber
 
Waveform
Exponential or square
Voltage, V
200–3,000
Capacitance, µF
10, 25, 50
Resistance (parallel), Ω
50–1,000 Ω in 50 Ω increments, plus infinity
Sample resistance, Ω
20 Ω minimum at 200–2,500 V; 600 Ω minimum at 2,500–3,000 V
Square-wave timing
0.05–5 ms pulse duration in 0.05 ms increments,
1–2 pulses, 5 sec minimum interval
Dimensions (W x D x H), cm
Main unit: 31 x 30 x 14
PC module: 31 x 28 x 5
Weight, kg
Main unit: 6.6
PC module: 1.9
Gene Pulser Xcell Main Unit
 
Waveform
Exponential or square
Voltage, V
200–3,000
Capacitance, µF
10, 25, 50
Sample resistance
20 Ω minimum at 200–2,500 V; 600 Ω minimum at 2,500–3,000 V
Square-wave timing
0.05–5 ms pulse duration in 0.05 ms increments,1–2 pulses, 5 sec minimum interval
Dimensions (W x D x H), cm
31 x 30 x 14
Weight, kg
6.6

Accessories

Accessories

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1652681
Pkg of 1, multiwell plate for use with the Gene Pulser MXcell electroporation system (#165-2670)
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1652682
Pkg of 1, multiwell plate for use with the Gene Pulser MXcell electroporation system (#165-2670)
165-2683_tn.jpg
1652683
Pkg of 1, multiwell plate for use with the Gene Pulser MXcell electroporation system (#165-2670)
165-2081_tn.jpg
1652081
Pkg of 5, 0.4 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells
165-2088_tn.jpg
1652088
Pkg of 50, 0.4 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells
165-2091_tn.jpg
1652091
Pkg of 500, 0.4 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells

Kits & Reagents

Kits & Reagents

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1652676
10 x 1.8 ml, electroporation buffer for mammalian, primary, and difficult-to-transfect cells, for use with any Gene Pulser electroporation system
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1652677
30 ml, electroporation buffer for mammalian, primary, and difficult-to-transfect cells, for use with any Gene Pulser Electroporation System

Documents

Documents

Number Description Options
5700
Instruction Manual, Gene Pulser MXcell Electroporation Guide, Rev A
10010739
Instruction Manual, Gene Pulser MXcell Electroporation System, Rev B
10010740
Instruction Manual, Gene Pulser MXcell Electroporation System Quick Guide, Rev A
10010741
Instruction Manual, Gene Pulser MXcell Electroporation System Pre Set Protocols, Rev A
10010742
Instruction Manual, Gene Pulser MXcell Electroporation System Plate, Rev A
5598
Gene Pulser MXcell Electroporation System Flier, Rev B
5634
Gene Pulser MXcell Electroporation System Brochure, Rev A
5858
The Gene Pulser MXcell Electroporation System Provides Reproducible Results in Electroporation Plates and Cuvettes With the Same Protocol, Rev A
5641
The Gene Pulser MXcell Electroporation System Delivers Consistent Results Required for Optimizing Delivery Protocols, Rev A
5603
Optimization of Electroporation Using Gene Pulser Electroporation Buffer and the Gene Pulser MXcell Electroporation System, Rev A
5622
Optimization of Electroporation Conditions With the Gene Pulser MXcell Electroporation System, Rev A
5686
Optimization of Electroporation Conditions for Jurkat Cells Using the Gene Pulser MXcell Electroporation System, Rev A
5687
Transfection of Mammalian Cells Using Preset Protocols on the Gene Pulser MXcell Electroporation System, Rev A
5720
Transfection of Neuroblastoma Cell Lines Using the Gene Pulser MXcell Electroporation System, Rev A
5704
Electroporation Conditions for Chinese Hamster Ovary Cells Using the Gene Pulser MXcell Electroporation System, Rev A
5733
Transfection of Chinese Hamster Ovary-Derived DG44 Cells Using the Gene Pulser MXcell Electroporation System, Rev A
5774
Delivery of siRNA by Electroporation Into Primary Human Neutrophils Using the Gene Pulser MXcell System, Rev A
5778
Electroporation Parameters for Transfection of HL-60 Leukocytic Cell Line With siRNA Using the Gene Pulser MXcell System, Rev B
5823
Electroporation of Primary Murine Mast Cells Using the Gene Pulser MXcell Electroporation System, Rev A
5842
Optimization of Electroporation Conditions for Two Different Burkitt Lymphoma Cell Lines Using the Gene Pulser MXcell System, Rev B
5860
Analysis of IL-4 Dependent Gene Expression in Namalwa Cells by siRNA Transfection: An Example of Pathway Analysis Using the Gene Pulser MXcell Electroporation System, Rev A
5904
Transfection of Mouse and Human Embryonic Stem Cells by Electroporation Using the Gene Pulser Mxcell™ System, Rev A
5935
Electroporation of Smooth Muscle Cells Using the Gene Pulser MXcell Electroporation System, Rev A
5942
Delivery of siRNA into B-Cell Lymphomas Using the Gene Pulser MXcell Electroporation System, Rev A