Image
Description
Description
The BioLogic DuoFlow™ family of chromatography systems offers flexibility with multiple system configurations, many optional upgrades, and a common software platform that is intuitive and easy to follow. These systems can be used on the laboratory bench or in a coldroom.
The BioLogic DuoFlow 10 system includes an F10 workstation for a flow rate of 0.01–10 ml/min* at 3,500 psi (233 bar, 23 MPa). A Model MX-1 mixer, 3-tray rack, AVR7-3 sample inject valve, fittings kit, UV detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit, UNO® Q1 column, and instructions are also included.
A Dell PC controller enables easy communication with the workstation and peripheral devices via an external USB Bitbus communicator. The controller includes the Windows XP operating system, application software, keyboard, mouse, and high-resolution flat-panel monitor.
BioLogic DuoFlow modular components allow the system to meet both laboratory space and application requirements; as requirements change, systems may be easily reconfigured and seamlessly upgraded with increased functionality, such as higher flow rates, sophisticated detection capabilities, pH monitoring, column scouting, and buffer blending.
* To double the flow rate to up to 20 ml/min, use the BioLogic DuoFlow Maximizer™ valve system. The optional F40 pump kit expands pumping capabilities to 40 ml/min.
Related Technologies
Related Products
Specifications
Specifications
Operating Limits
Flow rate
0.01–10 ml/min
Pressure
3,500 psi (23 MPa) at 10 ml/min
Detection
UV Detection
Standard 254 and 280 nm filters; optional 214, 365, 405, and 436 nm filters; standards 5 mm analytical flow cell; optional 2 mm preparative flow cell; optional BioLogic QuadTec UV/Vis detector for simultaneous 4-wavelength detection
Accessories
Accessories
Image
(Discontinued) F10 Pump Maintenance Kit
7600164
Kit includes 2 piston seals, 4 check valves, seal removal tool, 2 O-rings, to service one F10 pump
Image
(Discontinued) F10 Piston Seals
7600161
Pkg of 2, piston seals, includes seal tool, to service one F10 pump
Image
(Discontinued) F10 Piston Kit
7600162
Piston kit, includes 2 pistons, to service one F10 pump
Image
(Discontinued) Check Valve
7500162
Pkg of 1, check valve, for use with BioLogic DuoFlow workstation, (4 check valves required per pump)
Image
(Discontinued) Inline Filter Kit
7500703
Filter kit, includes 1 inline filter and 2 replacement frits, for use with BioLogic DuoFlow and NGC Systems
Image
(Discontinued) 40 psi Backpressure Regulator
7500230
Pkg of 1, for use with BioLogic DuoFlow and NGC Workstations
Image
(Discontinued) Bar Clamps
7500265
Pkg of 5, bar clamps, for use with BioLogic Rack (7500251)
Image
(Discontinued) Column Clamp Set
7500260
Column clamp set, includes 1 column clamp assembly, for use with BioLogic Rack (7500251)
Image
(Discontinued) Horizontal Bar Kit
7500264
Bar kit, includes 2 tie bars, 4 bar clamps, for use with BioLogic Rack (7500251)
Image
(Discontinued) Vertical Bars
7500263
Pkg of 2, 10 cm short bars, for use with BioLogic Rack (7500251)
Image
(Discontinued) Vertical Bars
7500262
Pkg of 2, 64 cm long bars, for use with BioLogic Rack (7500251)
Image
(Discontinued) BioLogic Rack Expansion Kit
7500268
Kit, includes 2 rack trays, 2 long vertical bars, 16 sleeves, for use with BioLogic Rack (7500251)
Image
(Discontinued) SVT3-2 Diverter Valve
7600410
Pkg of 1, solenoid valve, 3-port, 2-position, 30 psi (2 bar) limit, for use with BioLogic DuoFlow system
Image
(Discontinued) Sample Injection Port
7500471
Pkg of 1, sample injection port, for use with AVR7-3 automated sample inject valve (#760-0406)
Image
(Discontinued) Injection Needle
1250224
Pkg of 1, 22-gauge, injection needle, blunt, for use with BioLogic DuoFlow system
Image
(Discontinued) Small-Volume Sample Loop Kit
7500490
Pkg of 1, sample loop kit, includes 100, 250, and 500 μl PEEK loops
Image
(Discontinued) Large-Volume Sample Loop Kit
7500491
Pkg of 1, sample loop kit, includes 1, 2, and 5 ml PEEK loops
Image
(Discontinued) Model MX-1 Mixer
7600170
Pkg of 1, mixer, includes mixer body (263 μl) and standard mixer barrel for total volume of 750 μl
Image
(Discontinued) Gradient Pump Maintenance Kit
7500164
Pump maintenance kit, includes 2 piston seals, 2 check valves, 2 piston wipers, and piston seal tool
Image
(Discontinued) Piston Seals
7500161
Pkg of 4, piston seals
Image
(Discontinued) F10 Tubing Kit
7600650
High-pressure tubing kit, includes precut and fitted PTFE, Tefzel, and orange PEEK tubing, for installation of BioLogic DuoFlow basic chromatography system running at flow rates <40 ml/min
Image
(Discontinued) BioLogic Rack
7500251
Includes rack tray, 8 sleeves, 2 short vertical bars, 2 long vertical bars, column clamp set, 5 bar clamps, 4 cable organizers
Kits & Reagents
Kits & Reagents
Image
(Discontinued) BioLogic DuoFlow™ and NGC™ Fittings Kit
7600550
Fittings kit contains PEEK and Tefzel nuts, ferrules, unions, plugs, and luer syringe, for use with BioLogic DuoFlow and NGC systems
Image
(Discontinued) UNO Q1 Column
7200001
Pkg of 1, 7 x 35 mm, 1.3 ml, prepacked monolith ion exchange column, 20 mg max. protein load, 0.5–5.0 ml/min flow rate, operating pressure 700 psi
Image
(Discontinued) UNO Q6 Column
7200003
Pkg of 1, 12 x 53 mm, 6 ml, prepacked monolith ion exchange column, 90 mg max. protein load, 0.5–8.0 ml/min flow rate, operating pressure 700 psi
Image
(Discontinued) UNO Q12 Column
7200005
Pkg of 1, 15 x 68 mm, 12 ml, prepacked monolith ion exchange column, 180 mg max. protein load, 0.5–8.0 ml/min flow rate, operating pressure 700 psi
Image
(Discontinued) UNO S1 Column
7200021
Pkg of 1, 7 x 35 mm, 1.3 ml, prepacked monolith ion exchange column, 20 mg max. protein load, 0.5–5.0 ml/min flow rate, operating pressure 700 psi
Image
(Discontinued) UNO S6 Column
7200023
Pkg of 1, 12 x 53 mm, 6 ml, prepacked monolith ion exchange column, 90 mg max protein load, 0.5–8.0 ml/min flow rate, operating pressure 700 psi
Image
(Discontinued) UNO S12 Column
7200025
Pkg of 1, 15 x 68 mm, 12 ml, prepacked monolith ion exchange column, 180 mg max. protein load, 0.5–8.0 ml/min flow rate, operating pressure 700 psi
Image
(Discontinued) Bio-Scale™ Mini Ion Exchange Sample Pack
7324650
Includes one 1 ml cartridge each of UNOsphere™ S, UNOsphere Q, Macro-Prep® High Q, Macro-Prep High S, Macro-Prep DEAE
Image
(Discontinued) Bio-Scale Mini Affinity Sampler Pack
7324651
Includes one 1 ml cartridge each of IMAC and Affi-Prep protein A, one 5 ml cartridge each of DEAE Affi-Gel Blue and Affi-Gel Blue
(Discontinued) BioLogic System Starter Kit
7600135
Kit includes protein anion exchange standards, 50 µL injection loop, buffers, 1 mL syringe, and manual
Documents
Documents
Login Required Number Description Options
4106040
Instruction Manual, V9-8 Manual, and AV9-8 Automated Stream Select Valves, Rev C
6439
Improving Aggregrate Removal from a Monoclonal Antibody Feedstream Using High-Resolution Cation Exchange Chromatography, Rev B
DC7602030
Declaration of Conformity, BioLogic USB Bitbus Device, Rev A
2780
Purification of Murine IgG1 Using UNOsphere S and CHT Ceramic Hydroxyapatite Chromatography, Rev A
2849
Protein A Removal From IgG on CHT Ceramic Hydroxyapatite Support, Rev A
5342
Automated Three-Dimensional Purification Method for Histidine-Tagged Proteins Using the BioLogic DuoFlow Maximizer Chromatography System, Rev A
5334
A Method for Rapid, Large-Scale Removal of Albumin and IgG From Human Serum Using the BioLogic DuoFlow Chromatography System, Rev A
5251
Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins, Rev A
3039
Separation Reproducibility With the BioLogic Chromatography Systems
5326
Chromatography Column Connection Guide, Rev A
5369
Biologic DuoFlow System Family Brochure, Rev A
2584
BioLogic DuoFlow™ Valving Guide, Rev E
2815
BioLogic DuoFlow™ Valves Brochure, Rev C
2687
BioLogic DuoFlow Chromatography System Brochure, Rev D
4106041
Instruction Manual, BioLogic HR Valve Plumbing Diagrams, V7-3, AV7-3, Rev A
4106050
Instruction Manual, BioLogic™ HR Sample Inject Port Installation, V7-3, AV7-3 Valves, Rev B
4106054
Instruction Manual, BioLogic™ HR Gradient Pump Maintenance Kit, Rev C
4106248
Instruction Manual, BioLogic DuoFlow Chromatography System Software Version 5.0, Rev A
4006210
Instruction Manual, BioLogic™ Chromatography System
4106171
Instruction Manual, BioLogic DuoFlow Software Version 3.0 Discrepancies Solutions, Rev A
4106181
Instruction Manual, BioLogic DuoFlow Chromatography System Version 4.0 Software New Features, Rev A
4006208
Instruction Manual, BioLogic DuoFlow™ Chromatography System Starter Kit, Rev B
4106249
Instruction Manual, BioLogic DuoFlow Chromatography System 5.0 Software New Features, Rev A
4106039
Instruction Manual, BioLogic Column Rack Expansion Kit, Rev A
4106525
Instruction Manual Insert, BioLogic pH Monitor, Rev B
4106523
Instruction Manual Insert, BioLogic DuoFlow System USB Bitbus Communicator, Rev B
4106520
Instruction Manual Insert, BioLogic™ DuoFlow F10 Tubing Kit, Rev C
4006229
Instruction Manual, BioLogic DuoFlow™ Chromatography System, Rev B
