Real-Time PCR Supermixes and Kits

Bio-Rad's supermixes can make any qPCR experiment easier, faster, and more
effective. Our real-time PCR supermixes are designed for:

  • Any instrument — universal reference dye is compatible with all qPCR platforms
  • Any chemistry — supermixes for SYBR Green or probe-based detection chemistry
  • Any conditions — our patented Sso7d fusion polymerase* guarantees superior qPCR performance under varying and/or challenging conditions

qPCR Supermixes

 
SsoAdvanced Universal Supermixes
iTaq Universal Supermixes
 
SsoAdvanced Universal SYBR Green Supermix
SsoAdvanced Universal SYBR Green Supermix
SsoAdvanced Universal Probes Supermix
SsoAdvanced Universal Probes Supermix
SsoAdvanced Universal Inhibitor-Tolerant Supermix
SsoAdvanced Universal Inhibitor-Tolerant Supermix
iTaq Universal SYBR Green Supermix
iTaq Universal SYBR Green Supermix
iTaq Universal Probes Supermix
iTaq Universal Probes Supermix
Features
Robust performance with demanding templates and under challenging conditions
Optimized for crude samples containing PCR inhibitors
Advanced formulation for reliable, reproducible gene expression analysis on any qPCR instrument
Detection chemistry SYBR Green Probes SYBR Green SYBR Green Probes
Sso7d fusion polymerase Yes Yes Yes No No
Sensitive detection of low-abundance targets
Efficient amplification of difficult targets
Specific amplification of targets
Robust performance under varying PCR conditions**
Tolerance to PCR inhibitors
Cycling protocol compatibility Both standard and fast
Instrument compatibility Any instrument
Documents Product Insert Product Insert Product Insert
* U.S. patents 6,627,424; 7,541,170; and 7,560,260.
** Primer concentrations, annealing temperatures, salt concentrations, and/or cycling protocol (standard or fast)

iQ and SsoFast PCR Supermixes

iQ and SsoFast PCR Supermixes have been supporting researchers performing basic real-time PCR analysis for years. If you have not yet used them, we recommend trying our new SsoAdvanced Universal SYBR Green and SsoAdvanced Universal Probes Supermixes as superior-performance alternatives for use on any real-time PCR instrument.

Resources

Understanding Real-Time PCR Supermixes

This interactive tutorial provides an introduction to the chemistry behind real-time PCR reactions and supermixes as well as a protocol for comparing different reagents.

 

Videos

Applications & Technologies

  • qPCR/Real-Time PCR Reagents

    qPCR/Real-Time PCR Reagents

    Guidelines for selecting the appropriate reagents for your real-time PCR-based application

  • Real-Time PCR Data Analysis

    Real-Time PCR Data Analysis

    An overview of our CFX Manager™ Real-Time PCR Analysis Software features, including high resolution melt (HRM) analysis capabilities

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