iTaq Universal SYBR Green Supermix

Provides unmatched specificity and reaction efficiency for both genomic DNA and cDNA targets in qPCR on any real-time PCR instrument.

Ordering information

Overview

Our Universal Real-Time PCR Reagents Web App brings you in-depth product information, individual and competitive performance data, interactive tutorials, 3D animated videos, technical manuals and more.

iTaq Universal SYBR Green Supermix is a 2x concentrated, ready-to-use reaction master mix optimized for dye-based quantitative PCR (qPCR) on any real-time PCR instrument. It contains antibody-mediated hot-start iTaq DNA polymerase, dNTPs, MgCl₂, SYBR Green I dye, enhancers, stabilizers, and a blend of passive reference dyes (including ROX and fluorescein).

iTaq Universal SYBR Green Supermix lets you:

Obtain reliable and reproducible gene expression analysis results
Amplify genomic DNA and difficult amplicons with superior efficiency
Achieve consistent results across real-time instrument platforms under fast or standard conditions

iTaq Universal SYBR Green Supermix contains:

Advanced buffer formulation with SYBR Green I for optimal qPCR performance
Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)
Blend of passive reference dyes for universal real-time instrument compatibility

Instrument Compatibility

iTaq Universal SYBR Green Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

Systems

Applied Biosystems	StepOne/StepOnePlus 7500, ViiA 7 7000, 7300, 7700, 7900HT, QuantStudio Systems
Stratagene	Mx3000P, 3005P, 4000
Eppendorf	Mastercycler ep realplex 2, 4
QIAGEN	Corbett Rotor-Gene 3000, 6000, Q
Roche	LightCycler 480, 96 LightCycler 1.0, 1.5, 2.0*
BioFire	LightScanner HR-1 LightScanner 32*

* BSA must be added according to instrument specifications

Applications and Uses

Gene expression analysis
Genotyping
Mutation detection
Pathogen detection

Specifications

Storage at –20°C

Guaranteed for 12 months protected from light in a constant temperature freezer

Repeated freeze/thaw cycles

Up to 10 times

Storage at 4°C

Guaranteed for 3 months protected from light

Polymerase

iTaq DNA polymerase

Hot-start mechanism

Antibody mediated

Hot-start activation

95°C, 30 sec

Supermix concentration

Thermal cycling protocol

Standard and fast

Instrument compatibility

Validated on all Bio-Rad and ROX-dependent Applied Biosystems real-time PCR instruments and Roche LightCycler 480, QIAGEN Rotor-Gene Q, Eppendorf Mastercycler ep realplex, and Stratagene Mx real-time PCR systems

Template compatibility

cDNA, genomic DNA, plasmid DNA

Detection method

SYBR^® Green

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(Discontinued) iTaq™ Universal SYBR^® Green Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)

1725120

200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl₂, SYBR^® Green I, enhancers, stabilizers, and a blend of passive reference dyes

(Discontinued) iTaq™ Universal SYBR^® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725121

500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl₂, SYBR^® Green I, enhancers, stabilizers, and a blend of passive reference dyes

(Discontinued) iTaq™ Universal SYBR^® Green Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml)

1725122

1,000 x 20 µl reactions, 10 ml (10 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl₂, SYBR^® Green I, enhancers, stabilizers, and a blend of passive reference dyes

(Discontinued) iTaq™ Universal SYBR^® Green Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml)

1725124

2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl₂, SYBR^® Green I, enhancers, stabilizers, and a blend of passive reference dyes

(Discontinued) iTaq™ Universal SYBR^® Green Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml)

1725125

5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl₂, SYBR^® Green I, enhancers, stabilizers, and a blend of passive reference dyes

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