On This Page |
One-Step RT-qPCR Reagents | Cell Lysis RT-qPCR Kits | Resources | Applications & Technologies | Related Products |
Consult with a specialist about how Bio-Rad can help you with your genomics research.
Contact an Expert
Earn a free one-year service contract on your CFX System with purchase of Reagents
CFX Loyalty ProgramBio-Rad offers robust and reliable solutions to amplify RNA targets on all qPCR platforms including both ROX-dependent and ROX-independent systems. Our reagents feature:
- Easy reaction set up with 1- or 2-tube formats
- Sensitive, reproducible multiplex of up to 5 targets
- Room temperature stability of assembled reaction up to 24 hours
- Wide instrument compatibility
One-Step RT-qPCR Reagents
 Reliance One-Step Multiplex Supermix |
 iTaq Universal One-Step Kit |
|
|---|---|---|
| Features |
Single-tube, 4x supermix for 5-plex with 24-hour stability |
Easy 2-tube system for SYBR- and probe- based RT-qPCR |
| Ideal for |
|
|
| Starting material | Purified RNA or DNA, lysate up to 10 μl | Purified RNA, lysate up to 4 μl |
| Product format | 1 tube (4x RT-qPCR supermix) |
2 tubes (Reverse transcriptase + 2x qPCR supermix) |
| Detection chemistry | Probes, multiplex up to 5 targets | SYBR Green or probes duplex |
| Detection sensitivity | High | High |
| Tolerant to inhibitors | ✓ Great |
✓ Good |
| Assembled reaction stability | ✓ Up to 24 hours |
|
| Instrument compatibility | Both ROX-dependent (high- and low-) and -independent instruments |
Both ROX-dependent (high- and low-) and -independent instruments |
| Documents | ||
| Order Now | Order Now |
Cell Lysis RT-qPCR Kits
SingleShot Cell Lysis RT-qPCR Kits allow RT-qPCR to be performed directly from cultured cells without RNA purification. Kits are available for both one-step and two-step RT-qPCR with SYBR Green and probe chemistry.
Resources
Understanding Real-Time PCR
This interactive tutorial provides an introduction to real-time PCR basics: why it’s important, what situations are best applications for real-time PCR, and what types of data you can obtain.
Real-Time qPCR Reagents Brochure
(PDF 4.3MB)
Amplification Reagents and Plastics Brochure
(PDF 6.1MB)
Reagent Comparison Guide for Real-Time PCR
(PDF 3MB)
Videos
Applications & Technologies
Codetection of Viral RNA and DNA Using One-Step Multiplex RT-qPCR
Perform efficient, sensitive multiplex pathogen detection with Reliance Once-Step Multiplex Supermix
What is Gene Expression Analysis?
An introduction to gene regulation mechanisms, methods for gene expression profiling, and quantitation techniques including real-time PCR
qPCR/Real-Time PCR Reagents
Guidelines for selecting the appropriate reagents for your real-time PCR-based application
lncRNA RT-qPCR Workflow
This innovative workflow enables highly sensitive detection of lncRNAs and helps to overcome challenges associated with lncRNA expression analysis
Real-Time PCR Doctor
Having trouble with real-time PCR experiments? The Real-Time PCR Doctor can help you diagnose and correct common qPCR problems
Related Products
PrimePCR Validated PCR Assays
Experimentally validated PCR primer and probe assays for real-time PCR and Droplet Digital PCR.
SingleShot Cell Lysis Kits
Rapidly generate cell lysates that are suitable for RT-qPCR analysis without RNA purification.
CFX Automation System II
Works with all CFX Real-Time PCR Systems to facilitate automation of workflows, generation of large volumes of data, and rapid data analysis.
PCR Plastic Consumables
PCR tubes, plates, seals, and accessories precisely manufactured for various PCR applications.
Real-Time PCR Detection Systems
An automation-compatible, gradient-enabled thermal cycler with interchangeable optical reaction modules for singleplex or multiplex real-time PCR.
Real-Time PCR Supermixes and Kits
Designed for superior qPCR performance with any detection chemistry and on any instrument.
cDNA Synthesis Kits
Reverse transcription kits for easy setup, high reproducibility, and bias-free cDNA synthesis using an optimum blend of primers.
Custom PCR Reagent Service
A range of customization options for our existing products are available