
1-Step RT-qPCR from RNA or Cells
Product Overview
Bio-Rad offers robust and reliable solutions to amplify RNA targets on all qPCR platforms including both ROX-dependent and ROX-independent systems. Our reagents feature:
- Easy reaction set up with 1- or 2-tube formats
- Sensitive, reproducible multiplex of up to 5 targets
- Room temperature stability of assembled reaction up to 24 hours
- Wide instrument compatibility

CFX Loyalty Program
Earn a free one-year service contract on your CFX System with purchase of Reagents
One-Step RT-qPCR Reagents
![]() Reliance One-Step Multiplex Supermix |
![]() iTaq Universal One-Step Kit |
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Features |
Single-tube, 4x supermix for 5-plex with 24-hour stability |
Easy 2-tube system for SYBR- and probe- based RT-qPCR |
Ideal for |
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Starting material | Purified RNA or DNA, lysate up to 10 μl | Purified RNA, lysate up to 4 μl |
Product format | 1 tube (4x RT-qPCR supermix) |
2 tubes (Reverse transcriptase + 2x qPCR supermix) |
Detection chemistry | Probes, multiplex up to 5 targets | SYBR Green or probes duplex |
Detection sensitivity | High | High |
Tolerant to inhibitors | ✓ Great |
✓ Good |
Assembled reaction stability | ✓ Up to 24 hours |
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Instrument compatibility | Both ROX-dependent (high- and low-) and -independent instruments |
Both ROX-dependent (high- and low-) and -independent instruments |
Documents | ||
Ordering | Order Now Reliance One-Step Multiplex Supermix | Order Now |
Cell Lysis Kits

Cell Lysis RT-qPCR Kits
SingleShot Cell Lysis RT-qPCR Kits allow RT-qPCR to be performed directly from cultured cells without RNA purification. Kits are available for both one-step and two-step RT-qPCR with SYBR Green and probe chemistry.
Resources

Understanding Real-Time PCR
This interactive tutorial provides an introduction to real-time PCR basics: why it’s important, what situations are best applications for real-time PCR, and what types of data you can obtain.
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Real-Time qPCR Reagents Brochure
(PDF 4.3MB) -
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Amplification Reagents and Plastics Brochure
(PDF 6.1MB) -
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Reagent Comparison Guide for Real-Time PCR
(PDF 3MB)
Videos
Applications & Technologies
Codetection of Viral RNA and DNA Using One-Step Multiplex RT-qPCR
Perform efficient, sensitive multiplex pathogen detection with Reliance Once-Step Multiplex Supermix
What is Gene Expression Analysis?
An introduction to gene regulation mechanisms, methods for gene expression profiling, and quantitation techniques including real-time PCR
qPCR/Real-Time PCR Reagents
Guidelines for selecting the appropriate reagents for your real-time PCR-based application
lncRNA RT-qPCR Workflow
This innovative workflow enables highly sensitive detection of lncRNAs and helps to overcome challenges associated with lncRNA expression analysis
Real-Time PCR Doctor
Having trouble with real-time PCR experiments? The Real-Time PCR Doctor can help you diagnose and correct common qPCR problems