1-Step RT-qPCR from RNA or Cells
Product Overview
Bio-Rad offers robust and reliable solutions to amplify RNA targets on all qPCR platforms including both ROX-dependent and ROX-independent systems. Our reagents feature:
- Easy reaction set up with 1- or 2-tube formats
- Sensitive, reproducible multiplex of up to 5 targets
- Room temperature stability of assembled reaction up to 24 hours
- Wide instrument compatibility
CFX Loyalty Program
Earn a free one-year service contract on your CFX System with purchase of Reagents
One-Step RT-qPCR Reagents
 Reliance One-Step Multiplex Supermix |
 iTaq Universal One-Step Kit |
|
|---|---|---|
| Features |
Single-tube, 4x supermix for 5-plex with 24-hour stability |
Easy 2-tube system for SYBR- and probe- based RT-qPCR |
| Ideal for |
|
|
| Starting material | Purified RNA or DNA, lysate up to 10 μl | Purified RNA, lysate up to 4 μl |
| Product format | 1 tube (4x RT-qPCR supermix) |
2 tubes (Reverse transcriptase + 2x qPCR supermix) |
| Detection chemistry | Probes, multiplex up to 5 targets | SYBR Green or probes duplex |
| Detection sensitivity | High | High |
| Tolerant to inhibitors | ✓ Great |
✓ Good |
| Assembled reaction stability | ✓ Up to 24 hours |
|
| Instrument compatibility | Both ROX-dependent (high- and low-) and -independent instruments |
Both ROX-dependent (high- and low-) and -independent instruments |
| Documents | ||
| Ordering | Order Now Reliance One-Step Multiplex Supermix | Order Now |
Cell Lysis Kits
Cell Lysis RT-qPCR Kits
SingleShot Cell Lysis RT-qPCR Kits allow RT-qPCR to be performed directly from cultured cells without RNA purification. Kits are available for both one-step and two-step RT-qPCR with SYBR Green and probe chemistry.
Resources
Understanding Real-Time PCR
This interactive tutorial provides an introduction to real-time PCR basics: why it’s important, what situations are best applications for real-time PCR, and what types of data you can obtain.
-
Image
Real-Time qPCR Reagents Brochure
(PDF 4.3MB) -
Image
Amplification Reagents and Plastics Brochure
(PDF 6.1MB) -
Image
Reagent Comparison Guide for Real-Time PCR
(PDF 3MB)
Videos
Applications & Technologies
Codetection of Viral RNA and DNA Using One-Step Multiplex RT-qPCR
Perform efficient, sensitive multiplex pathogen detection with Reliance Once-Step Multiplex Supermix
What is Gene Expression Analysis?
An introduction to gene regulation mechanisms, methods for gene expression profiling, and quantitation techniques including real-time PCR
qPCR/Real-Time PCR Reagents
Guidelines for selecting the appropriate reagents for your real-time PCR-based application
lncRNA RT-qPCR Workflow
This innovative workflow enables highly sensitive detection of lncRNAs and helps to overcome challenges associated with lncRNA expression analysis
Real-Time PCR Doctor
Having trouble with real-time PCR experiments? The Real-Time PCR Doctor can help you diagnose and correct common qPCR problems
