1-Step RT-qPCR from RNA or Cells

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Bio-Rad offers robust and reliable solutions to amplify RNA targets on all qPCR platforms including both ROX-dependent and ROX-independent systems. Our reagents feature:

  • Easy reaction set up with 1- or 2-tube formats
  • Sensitive, reproducible multiplex of up to 5 targets
  • Room temperature stability of assembled reaction up to 24 hours
  • Wide instrument compatibility

One-Step RT-qPCR Reagents

Reliance One-Step Multiplex Supermix
Reliance One-Step Multiplex Supermix
iScript gDNA Clear cDNA Synthesis Kit
iTaq Universal One-Step Kit
Single-tube, 4x supermix for 5-plex with 24-hour stability
Easy 2-tube system for SYBR- and probe- based RT-qPCR
Ideal for
  • Multiplexing up to 5 targets
  • Viral/pathogen detection
  • High throughput screening
  • Diagnostic research
  • Gene expression
  • Viral/pathogen detection
  • Diagnostic research
  • Gene expression
  • Multiplexing up to 2 targets
Starting material Purified RNA or DNA, lysate up to 10 μl Purified RNA, lysate up to 4 μl
Product format 1 tube
(4x RT-qPCR supermix)
2 tubes
(Reverse transcriptase + 2x qPCR supermix)
Detection chemistry Probes, multiplex up to 5 targets SYBR Green or probes duplex
Detection sensitivity High High
Tolerant to inhibitors

Assembled reaction stability
Up to 24 hours
Instrument compatibility Both ROX-dependent (high- and low-)
and -independent instruments
Both ROX-dependent (high- and low-)
and -independent instruments
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Cell Lysis RT-qPCR Kits

SingleShot Cell Lysis RT-qPCR Flow

SingleShot Cell Lysis RT-qPCR Kits allow RT-qPCR to be performed directly from cultured cells without RNA purification. Kits are available for both one-step and two-step RT-qPCR with SYBR Green and probe chemistry.

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Understanding Real-Time PCR

This interactive tutorial provides an introduction to real-time PCR basics: why it’s important, what situations are best applications for real-time PCR, and what types of data you can obtain.



Applications & Technologies



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