INTRODUCINGThe Reliance Select cDNA Synthesis Kit
Overcome challenging RT-qPCR samples in a single assay.
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Reverse transcription is a crucial step for gene expression studies, sequencing, cloning, and other applications. Optimization of this step is a priority to researchers to ensure the best results downstream. We offer a variety of cDNA synthesis kits to meet researchers' needs, including high fidelity or dealing with challenging samples, difficult targets, and rare or long transcripts.
cDNA Synthesis Kits
The Reliance Select Kit enables researchers to synthesize cDNA with the highest level of sensitivity and robustness by utilizing the unique properties of its novel chimeric reverse transcriptase.
- Detection of very rare transcripts
- Processing of of samples in complex matrices, such as FFPE samples
- Overcoming difficult inhibitors
- Challenging targets with secondary structures and high GC concentration
iScript Reverse Transcription Reagents feature an RNase H+ MMLV reverse transcriptase plus an RNase inhibitor for reliable yields. Choose the best cDNA Synthesis kit for your application:
- One-tube supermix
- gDNA clearance
- High RNA input
- High fidelity
This interactive tutorial provides an introduction to reverse transcription basics, an explanation of how different reagents work, and a protocol for comparing the results with different reverse transcription reagents.
Applications & Technologies
Guidelines for selecting the appropriate reagents for your real-time PCR-based application
An introduction to gene regulation mechanisms, methods for gene expression profiling, and quantitation techniques including real-time PCR
An innovative workflow that enables highly sensitive detection of lncRNAs and helps to overcome challenges associated with lncRNA expression analysis
Having trouble with real-time PCR experiments? The Real-Time PCR Doctor can help you diagnose and correct common qPCR problems
PCR and real-time PCR reagents with advanced enzyme engineering and formulations optimized for a wide variety of templates and target sequences
For robust reverse transcription and SYBR Green or probe-based qPCR in one step on any instrument
An automation-compatible, gradient-enabled thermal cycler with interchangeable optical reaction modules for singleplex or multiplex real-time PCR
Complete and fast solution for one- or two-step qPCR reactions directly from cell lysates with no RNA purification step
Personal units and flexible modular thermal cyclers provide low- to high-throughput capabilities for all your PCR amplification needs
Ready-to-use supermix for unbiased, target-specific preamplification of cDNA or gDNA using up to 100 qPCR assays
A large selection of thin-wall polypropylene PCR tubes, PCR plates, seals, and accessories precisely manufactured for various PCR applications