ReadyStrip™ IPG Strips

ReadyStrip™ IPG Strips

IPG strips simplify first-dimension separations by immobilizing the pH gradient on an easy-to-handle support strip. ReadyStrip IPG strips are cast using high-purity IPG monomers.

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Ordering Information

Overview

IPG strips simplify first-dimension separations by immobilizing the pH gradient on an easy-to-handle support strip. ReadyStrip™ IPG strips are cast using high-purity IPG monomers and are thoroughly tested for quality and performance to deliver gel-to-gel reproducibility. ReadyStrip IPG strips, available in a wide selection of pH gradients and strip lengths from 7 to 24 cm, fit Bio-Rad vertical electrophoresis cells and gels.

Design Features

  • Stringent gel length tolerances of ±2 mm for consistent pI separations
  • Anode and pH range clearly printed on each strip, with bar coding on 24 cm strips
  • Consistent backing lengths on both ends for self-centering on second-dimension gels without trimming

Gradient Selection

  • Standard broad-range pH gradients for maximum resolution of total protein on a single gel
  • Narrow-range gradients for greater resolution with more cm gel/pH unit over a full pH 3–10 range
  • Micro-range gradients for the ultimate in resolution — zoom in on an area of interest or fine-map an entire proteome; strips are carefully designed with sufficient overlap to allow spot matching while limiting the extent of redundant data
  • Comprehensive offering increases resolving power in the first dimension with overlapping pH ranges

Relative Focusing Power

Relative focusing power describes the enhanced resolution expected in the first dimension when using IPG strips of different lengths or pH ranges. A 7 cm pH 3–10 IPG strip is arbitrarily assigned a baseline focusing power of 1.0 in order to calculate the relative focusing power of the other strips, as shown here.

Narrow-Range Strips

Narrow-range strips provide an increase in resolution. As illustrated in figures A–D, three overlapping narrow-range strips can dramatically expand the region for analysis.

Micro-Range Strips

Micro-range IPG strips enable the highest resolution available for IPG strips. The micro-range strips cover as small a range as 1 pH unit. These are particularly well suited for resolving different isoforms of the same protein.

Ordering

items

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1632000
Pkg of 12, 7 cm, pH 3–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632001
Pkg of 12, 7 cm, pH 4–7, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632002
Pkg of 12, 7 cm, pH 3–10 nonlinear, immobilized pH gradient (IPG) strips for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632003
Pkg of 12, 7 cm, pH 3–6, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632004
Pkg of 12, 7 cm, pH 5–8, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632005
Pkg of 12, 7 cm, pH 7–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632028
Pkg of 12, 7 cm, pH 3.9–5.1, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632029
Pkg of 12, 7 cm, pH 4.7–5.9, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632031
Pkg of 12, 7 cm, pH 6.3–8.3, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632014
Pkg of 12, 11 cm, pH 3–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632015
Pkg of 12, 11 cm, pH 4–7, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632016
Pkg of 12, 11 cm, pH 3–10 nonlinear, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632017
Pkg of 12, 11 cm, pH 3–6, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632018
Pkg of 12, 11 cm, pH 5–8, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632019
Pkg of 12, 11 cm, pH 7–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632024
Pkg of 12, 11 cm, pH 3.9–5.1, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632027
Pkg of 12, 11 cm, pH 6.3–8.3, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632007
Pkg of 12, 17 cm, pH 3–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632008
Pkg of 12, 17 cm, pH 4–7, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632009
Pkg of 12, 17 cm, pH 3–10 nonlinear, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632010
Pkg of 12, 17 cm, pH 3–6, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632011
Pkg of 12, 17 cm, pH 5–8, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632012
Pkg of 12, 17 cm, pH 7–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632020
Pkg of 12, 17 cm, pH 3.9–5.1, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632022
Pkg of 12, 17 cm, pH 5.5–6.7, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632032
Pkg of 12, 18 cm, pH 3–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632033
Pkg of 12, 18 cm, pH 3–10 nonlinear, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632034
Pkg of 12, 18 cm, pH 4–7, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632035
Pkg of 12, 18 cm, pH 3–6, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632036
Pkg of 12, 18 cm, pH 5–8, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632037
Pkg of 12, 18 cm, pH 7–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632038
Pkg of 12, 18 cm, pH 3.9–5.1, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632039
Pkg of 12, 18 cm, pH 4.7–5.9, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632040
Pkg of 12, 18 cm, pH 5.5–6.7, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632041
Pkg of 12, 18 cm, pH 6.3–8.3, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632042
Pkg of 12, 24 cm, pH 3–10, immobilized pH gradient (IPG) strip with barcode for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632043
Pkg of 12, 24 cm, pH 3–10, nonlinear, immobilized pH gradient (IPG) strip with barcode for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632044
Pkg of 12, 24 cm, pH 4–7, immobilized pH gradient (IPG) strip with barcode for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632045
Pkg of 12, 24 cm, pH 3–6, immobilized pH gradient (IPG) strip with barcode for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632046
Pkg of 12, 24 cm, pH 5–8, immobilized pH gradient (IPG) strip with barcode for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632047
Pkg of 12, 24 cm, pH 7–10, immobilized pH gradient (IPG) strip with barcode for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632050
Pkg of 12, 24 cm, pH 5.5–6.7, immobilized pH gradient (IPG) strip with barcode for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632051
Pkg of 12, 24 cm, pH 6.3–8.3, immobilized pH gradient (IPG) strip with barcode for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

Kits & Reagents

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1632093
1 ml, ampholyte concentrate for use in IEF and with ReadyStrip IPG strips

Documents

Number Description Options
2442
ReadyStrip IPG Strip Product Information Sheet, Rev F
2621
PROTEAN Plus Dodeca Cell System Brochure, Rev C
6097
PROTEAN i12 IEF System Brochure, Ver B
2739
ReadyStrip IPG Strip Brochure
5353
Sample Preparation for 2-D Gel-Based Expression Proteomics of an Alzheimer's Disease Mouse Model (Poster), Rev A
6138
PROTEAN i12 IEF System: Independent Voltage and Current Control Enables Optimization of First-Dimension IEF Conditions, Rev A
6139
Versatile Separation Capabilities of the PROTEAN i12 IEF System, Rev A
6140
Use of the PROTEAN® i12 IEF System for In-Gel Peptide Fractionation Prior to LC-MS and Comparison with Off-Gel Fractionation, Rev A
10022548
Instruction Manual, PROTEAN i12 IEF Cell Quick Guide, Rev A
4006166
Instruction Manual, ReadyStrip IPG Strip
6493
Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide
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