Troubleshooting 2-D Electrophoresis Gels with 2-D Doctor

The 2-D Doctor is a self-help guide developed by Bio-Rad that enables you to identify and troubleshoot your 2-D gel issues. Comprehensive solutions and suggestions are provided to help solve 2-D gel electrophoresis challenges.

Related Topics: Protein Sample Preparation for 2-D Electrophoresis, First-Dimension Separation (Isoelectric Focusing), Second-Dimension Separation, Staining and Visualization of Proteins After 2-D Electrophoresis, Imaging and Analysis of 2-D Electrophoresis Gels and Protein Spot Excision and Protein Identification.

To start troubleshooting your 2-D gel problems choose the type of issue you are experiencing: horizontal streaking, vertical streaking or other.

 

I am having trouble with:

Horizontal Streaking Vertical Streaking Irregular Spot Patterns
 


Page Contents

Videos

2-D Video Tutorial
How to run a 2-D Gel from start to finish.
 

Documents

Number Description Options

TEST

Number Description Options
Number Description Options
6222 IPG Equilibration for the Second Dimension, Placement and Agarose Embedding of IPG Strips Click to download
6236 Using Precison Plus Protein Standard Plugs Click to download