Troubleshooting 2-D Electrophoresis Gels with 2-D Doctor

The 2-D Doctor is a self-help guide developed by Bio-Rad that enables you to identify and troubleshoot your 2-D gel issues. Comprehensive solutions and suggestions are provided to help solve 2-D gel electrophoresis challenges.

Related Topics: Protein Sample Preparation for 2-D Electrophoresis, First-Dimension Separation (Isoelectric Focusing), Second-Dimension Separation, Staining and Visualization of Proteins After 2-D Electrophoresis, Imaging and Analysis of 2-D Electrophoresis Gels and Protein Spot Excision and Protein Identification.

To start troubleshooting your 2-D gel problems choose the type of issue you are experiencing: horizontal streaking, vertical streaking or other.


I am having trouble with:

Horizontal Streaking Vertical Streaking Irregular Spot Patterns

Page Contents


2-D Video Tutorial
How to run a 2-D Gel from start to finish.


Number Description Options
2-D Electrophoresis Workflow How-To Guide, Rev F
High-Performance 2-D Gel Electrophoresis Using Narrow pH-Range ReadyStrip IPG Strips, Rev C
Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach
Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A
Focusing Strategy and Influence of Conductivity on Isoelectric Focusing in Immobilized pH Gradients, Rev A
Combination of 2-D Gel and Liquid-Phase Electrophoretic Separations As Proteomic Tools in Neuroscience, Rev A
Expression Proteomics Overview Brochure, Rev B
Sensitivity and Protein-to-Protein Consistency of Flamingo Fluorescent Gel Stain Compared to Other Fluorescent Stains (Poster), Rev A
PROTEAN i12 IEF System Brochure, Ver B
PROTEAN Plus Dodeca Cell System Brochure, Rev C
2-D Electrophoresis: Tools for Rapid, High-Resolution Protein Separations Brochure, Rev B
Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B
Imaging and Analysis: Tools for Acquisition and Analysis of Protein Expression Data Brochure, Rev B


Number Description Options
Number Description Options
6222 IPG Equilibration for the Second Dimension, Placement and Agarose Embedding of IPG Strips Click to download
6236 Using Precison Plus Protein Standard Plugs Click to download