Troubleshooting 2-D Electrophoresis Gels with 2-D Doctor

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Overview

The 2-D Doctor is a self-help guide developed by Bio-Rad that enables you to identify and troubleshoot your 2-D gel issues. Comprehensive solutions and suggestions are provided to help solve 2-D gel electrophoresis challenges.

Related Topics: Protein Sample Preparation for 2-D Electrophoresis, First-Dimension Separation (Isoelectric Focusing), Second-Dimension Separation, Staining and Visualization of Proteins After 2-D Electrophoresis, Imaging and Analysis of 2-D Electrophoresis Gels and Protein Spot Excision and Protein Identification.

To start troubleshooting your 2-D gel problems choose the type of issue you are experiencing: horizontal streaking, vertical streaking or other.

 

I am having trouble with:

Horizontal Streaking Vertical Streaking Irregular Spot Patterns
 

 

Related Content

 
2-D Video Tutorial
How to run a 2-D Gel from start to finish.
 
Literature
Number Description Download
2651 2-D Electrophoresis Workflow How-To Guide, Rev F Click to download
2587 High-Performance 2-D Gel Electrophoresis Using Narrow pH-Range ReadyStrip IPG Strips, Rev C Click to download
2670 Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach Click to download
2740 Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A Click to download
2778 Focusing Strategy and Influence of Conductivity on Isoelectric Focusing in Immobilized pH Gradients, Rev A Click to download
2859 Combination of 2-D Gel and Liquid-Phase Electrophoretic Separations As Proteomic Tools in Neuroscience, Rev A Click to download
3098 Expression Proteomics Overview Brochure, Rev B Click to download
5545 Sensitivity and Protein-to-Protein Consistency of Flamingo Fluorescent Gel Stain Compared to Other Fluorescent Stains (Poster), Rev A Click to download
6097 PROTEAN i12 IEF System Brochure, Ver B Click to download
2621 PROTEAN Plus Dodeca Cell System Brochure, Rev C Click to download
3095 2-D Electrophoresis: Tools for Rapid, High-Resolution Protein Separations Brochure, Rev B Click to download
3096 Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B Click to download
3097 Imaging and Analysis: Tools for Acquisition and Analysis of Protein Expression Data Brochure, Rev B Click to download
Number Description Options
6222 IPG Equilibration for the Second Dimension, Placement and Agarose Embedding of IPG Strips Click to download
6236 Using Precison Plus Protein Standard Plugs Click to download
 
 
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