Nuvia IMAC Resin is a next-generation affinity chromatography media, with ultra-high capacity over a range of pH and flow rates, that provides a wide experimental design space for many applications.
Nuvia IMAC Resin, based on Nuvia Beads, contains nitrilotriacetic acid (NTA) as the chelating ligand for di- or trivalent metal ions. The chemical structure of NTA permits highly selective binding of recombinant histidine-tagged proteins when charged with Ni2+ or other transition metals, such as Zn2+ or Cu2+.
Due to superior mass transfer characteristics, Nuvia IMAC Resin offers high capacity at high flow rates. The resin can be used under either nondenaturing or denaturing conditions.
- Best-in-class binding capacity at fast flow rates
- Rapid mass transfer kinetics
- Efficient resolution of biomolecules from crude feedstreams
- Stable at pH 2–14, providing excellent NaOH stability
For purifying biomolecules in a variety of workflows:
- Excellent single-step purity via affinity chromatography
- Quick, efficient separation of biomolecules
- Contaminant removal
- Robust base matrix
This IMAC resin is compatible with 8 M urea, 6 M guanidine HCl, 1 M NaOH, alcohol up to 20% (v/v), and with common detergents, salts, reducing agents, and all commonly used aqueous buffers.
Nuvia IMAC Resin is available in 25 ml, 100 ml, and 500 ml bottles.
Download the Nuvia IMAC Resin Instruction Manual for detailed column packing recommendations and protocols for optimal protein isolation and column regeneration, cleaning, sanitizing, and storage.
Nuvia IMAC Resin may be packed into a variety of columns to suit your application, such as spin columns, low-pressure Econo™ Columns, and medium-pressure columns. Bio-Rad also offers other resins for affinity purification.
This resin is also available in prepacked Nuvia™ IMAC Columns, which are ideal for use on the NGC™ Chromatography System or any other low- to medium-pressure chromatography system. We recommend secondary purification using desalting columns.