Nuvia™ IMAC Resin, 100 ml #7800801

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Overview

Pkg of 1, 100 ml, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography
Safety Data Sheet

Safety Data Sheet

Description

Description

Use high-capacity Nuvia IMAC Resin for purification of recombinant histidine-tagged and other metal binding proteins. Resin can be used for batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems.

  • Chelating ligand is nitrilotriacetic acid (NTA)
  • Stable across a wide pH range (2–14), with high NaOH stability
  • Works under native or denaturing conditions
  • Can be used for single-step purification from crude samples or contaminant removal

Nuvia IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.

Product Options

Package Size Catalog Number
25 ml 7800800
100 ml 7800801
500 ml 7800802
5 L 12003233
10 L 12002782

Prepacked Bio-Scale™ Mini Nuvia IMAC Cartridges with Ni-charged NTA are available.

Related Products

  • Native IMAC Buffer Kit (6200239) includes IMAC lysis, wash, and elution buffers
  • Profinity IMAC Resin, uncharged or Ni-charged IDA
  • Empty Bio-Scale Mini Cartridges, 1 ml (7324660) and 5 ml (7324661)
  • Poly-Prep® Chromatography Columns, hold up to 2 ml support
  • His Antibody (6200203)

Related Categories

Supporting Documents

Online Resources

Specifications

Specifications

Ligand
Nitrilotriacetic acid (NTA)
Form
50% slurry in 20% ethanol
Particle size
38–53 µm (mean 50 µm)
Capacity
≥ 18 µmol Cu2+/ml resin, 6x histidine-tagged proteins >40 mg/ml resin (dynamic binding capacity varies from protein to protein)
Maximum operating pressure
45 psi (3.1 bar)
Recommended flow rate
<500 cm/hr at 25°C
Operating temperature range
4–40°C
Autoclavability (121°C, 30 min)
Yes (in 0.1 M sodium acetate)
Storage
4°C or ambient
Shelf life
≥3 years at ambient temperature in 20% ethanol